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[Effects of interferon-gamma on tubular epithelial-myofibroblast transdifferentiation in vitro].
Sichuan Da Xue Xue Bao Yi Xue Ban. 2008 Nov; 39(6):895-9, 943.SD

Abstract

OBJECTIVE

To investigate the effects of interferon-gamma (IFN-gamma) on tubular epithelial-myofibroblast transdifferentiation (TEMT) induced by transforming growth factor (TGF-beta1).

METHODS

The normal rat kidney tubular epithelial cells (NRK52E) were cultured and divided into blank (NRK52E cells only) control group, TGF-beta1 (3 ng/mL) treated group, IFN-gamma (1000 IU/mL) treated group, and IFN-gamma inhibition group (TGF-beta1 3 ng/mL + IFN-gamma 200, 400, 600, 1000, 2000, 3000 IU/mL). After 72 hours of treatment, the morphology of cells was observed under phase-contrast microscopy and scanning electron microscopy. The expressions of a-smooth muscle actin (alpha-SMA) and connective tissue growth factor (CTGF) were detected by immunocytochemistry. Flowcytometry was employed to measure the percentage of alpha-SMA+ cells and the mean channel fluorescence (MCF). The expressions of alpha-SMA mRNA and CTGF mRNA were examined by reverse transcription-polymerase chain reaction analyses (RT-PCR). The level of collagen in the culture supernatant was measured by Enzyme-linked immunoadsordent assay (ELISA).

RESULTS

NRK52E cells cultured in the control group showed a classic cobblestone morphology. TGF-beta1 induced NRK52E cells to transdifferentiate into myofibroblast-like cells, which showed strong alpha-SMA immunostaining. The TGF-beta1 treated cells had higher percentage of a-SMA+ cells, MCF and alpha-SMA mRNA, increased CTGF mRNA expression, and ascended collagen III than the blank controls (P<0.05). IFN-gamma treated alone did not make any changes to the cell morphology, the expressions of alpha-SMA mRNA and CTGF mRNA and the level of collagen III (P>0.05). IFN-gamma exerted a strong inhibitory effect on the TEMT induced by TGF-beta1. With the increase of IFN-gamma, the percentage of alpha-SMA+ cells, the level of collagen III, and the expressions of alpha-SMA mRNA and CTGF mRNA decreased (P<0.05).

CONCLUSION

IFN-gamma inhibits the TEMT induced by TGF-beta1 and reduces the level of collagen III.

Authors+Show Affiliations

Department of Nephrology, West China Hospital, Sichuan University, Chengdu 610041, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

19253820

Citation

Li, Qin, et al. "[Effects of Interferon-gamma On Tubular Epithelial-myofibroblast Transdifferentiation in Vitro]." Sichuan Da Xue Xue Bao. Yi Xue Ban = Journal of Sichuan University. Medical Science Edition, vol. 39, no. 6, 2008, pp. 895-9, 943.
Li Q, Fan JM, Pu L, et al. [Effects of interferon-gamma on tubular epithelial-myofibroblast transdifferentiation in vitro]. Sichuan Da Xue Xue Bao Yi Xue Ban. 2008;39(6):895-9, 943.
Li, Q., Fan, J. M., Pu, L., Li, Z., Qin, W., & Su, B. H. (2008). [Effects of interferon-gamma on tubular epithelial-myofibroblast transdifferentiation in vitro]. Sichuan Da Xue Xue Bao. Yi Xue Ban = Journal of Sichuan University. Medical Science Edition, 39(6), 895-9, 943.
Li Q, et al. [Effects of Interferon-gamma On Tubular Epithelial-myofibroblast Transdifferentiation in Vitro]. Sichuan Da Xue Xue Bao Yi Xue Ban. 2008;39(6):895-9, 943. PubMed PMID: 19253820.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of interferon-gamma on tubular epithelial-myofibroblast transdifferentiation in vitro]. AU - Li,Qin, AU - Fan,Jun-ming, AU - Pu,Lei, AU - Li,Zi, AU - Qin,Wei, AU - Su,Bai-hai, PY - 2009/3/4/entrez PY - 2009/3/4/pubmed PY - 2010/3/3/medline SP - 895-9, 943 JF - Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition JO - Sichuan Da Xue Xue Bao Yi Xue Ban VL - 39 IS - 6 N2 - OBJECTIVE: To investigate the effects of interferon-gamma (IFN-gamma) on tubular epithelial-myofibroblast transdifferentiation (TEMT) induced by transforming growth factor (TGF-beta1). METHODS: The normal rat kidney tubular epithelial cells (NRK52E) were cultured and divided into blank (NRK52E cells only) control group, TGF-beta1 (3 ng/mL) treated group, IFN-gamma (1000 IU/mL) treated group, and IFN-gamma inhibition group (TGF-beta1 3 ng/mL + IFN-gamma 200, 400, 600, 1000, 2000, 3000 IU/mL). After 72 hours of treatment, the morphology of cells was observed under phase-contrast microscopy and scanning electron microscopy. The expressions of a-smooth muscle actin (alpha-SMA) and connective tissue growth factor (CTGF) were detected by immunocytochemistry. Flowcytometry was employed to measure the percentage of alpha-SMA+ cells and the mean channel fluorescence (MCF). The expressions of alpha-SMA mRNA and CTGF mRNA were examined by reverse transcription-polymerase chain reaction analyses (RT-PCR). The level of collagen in the culture supernatant was measured by Enzyme-linked immunoadsordent assay (ELISA). RESULTS: NRK52E cells cultured in the control group showed a classic cobblestone morphology. TGF-beta1 induced NRK52E cells to transdifferentiate into myofibroblast-like cells, which showed strong alpha-SMA immunostaining. The TGF-beta1 treated cells had higher percentage of a-SMA+ cells, MCF and alpha-SMA mRNA, increased CTGF mRNA expression, and ascended collagen III than the blank controls (P<0.05). IFN-gamma treated alone did not make any changes to the cell morphology, the expressions of alpha-SMA mRNA and CTGF mRNA and the level of collagen III (P>0.05). IFN-gamma exerted a strong inhibitory effect on the TEMT induced by TGF-beta1. With the increase of IFN-gamma, the percentage of alpha-SMA+ cells, the level of collagen III, and the expressions of alpha-SMA mRNA and CTGF mRNA decreased (P<0.05). CONCLUSION: IFN-gamma inhibits the TEMT induced by TGF-beta1 and reduces the level of collagen III. SN - 1672-173X UR - https://www.unboundmedicine.com/medline/citation/19253820/[Effects_of_interferon_gamma_on_tubular_epithelial_myofibroblast_transdifferentiation_in_vitro]_ L2 - https://antibodies.cancer.gov/detail/CPTC-TGFB1-1 DB - PRIME DP - Unbound Medicine ER -