Tags

Type your tag names separated by a space and hit enter

Relative changes in tocopherols, isoflavones, total phenolic content, and antioxidative activity in soybean seeds at different reproductive stages.
J Agric Food Chem. 2009 Apr 08; 57(7):2705-10.JA

Abstract

Immature seeds of soybean are becoming increasingly popular as a snack/vegetable to harness the health benefits of soybean. They are shelled from the immature pods picked from the mother plant at different reproductive stages. Information concerning changes in antioxidant constituents and antioxidant capacity during reproductive phases of soybean seeds is scarce. The aim of the present study was to determine whether immature seeds picked at different reproductive stages differ in tocopherol, isoflavone, total phenolic contents, free radical scavenging activity, and total antioxidative capacity. Seeds shelled from the soybean pods picked at three reproductive stages (R5, R6, and R7) as well as at full maturity were subjected to high-performance liquid chromatography analyses for tocopherol and isoflavone contents. Significantly higher values (P<0.05) were observed for tocopherols and isoflavones in immature seeds picked at late reproductive stages. At the first reproductive stage, that is, R5 stage, delta-tocopherol was the predominant form of tocopherol, whereas in subsequent reproductive stages as well as at complete maturity stage, the gamma-isomer contributed maximum proportion to the total tocopherol content. Genistein was, in general, the major form of isoflavone at all reproductive stages. Reduction in free radical scavenging activity, total antioxidant capacity, and total phenolic content in late-picked seeds concomitant with increased concentration of tocopherol and isoflavone isomers was observed. The results show that bioactive constituents other than isoflavones and tocopherols may decline with the advancement of maturity.

Authors+Show Affiliations

National Research Centre for Soybean (Indian Council of Agricultural Research), Khandwa Road, Indore, M.P., India 452 017. vineetksahni@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

19256542

Citation

Kumar, Vineet, et al. "Relative Changes in Tocopherols, Isoflavones, Total Phenolic Content, and Antioxidative Activity in Soybean Seeds at Different Reproductive Stages." Journal of Agricultural and Food Chemistry, vol. 57, no. 7, 2009, pp. 2705-10.
Kumar V, Rani A, Dixit AK, et al. Relative changes in tocopherols, isoflavones, total phenolic content, and antioxidative activity in soybean seeds at different reproductive stages. J Agric Food Chem. 2009;57(7):2705-10.
Kumar, V., Rani, A., Dixit, A. K., Bhatnagar, D., & Chauhan, G. S. (2009). Relative changes in tocopherols, isoflavones, total phenolic content, and antioxidative activity in soybean seeds at different reproductive stages. Journal of Agricultural and Food Chemistry, 57(7), 2705-10. https://doi.org/10.1021/jf803122a
Kumar V, et al. Relative Changes in Tocopherols, Isoflavones, Total Phenolic Content, and Antioxidative Activity in Soybean Seeds at Different Reproductive Stages. J Agric Food Chem. 2009 Apr 8;57(7):2705-10. PubMed PMID: 19256542.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Relative changes in tocopherols, isoflavones, total phenolic content, and antioxidative activity in soybean seeds at different reproductive stages. AU - Kumar,Vineet, AU - Rani,Anita, AU - Dixit,Amit Kumar, AU - Bhatnagar,Deepak, AU - Chauhan,G S, PY - 2009/3/5/entrez PY - 2009/3/5/pubmed PY - 2009/6/16/medline SP - 2705 EP - 10 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 57 IS - 7 N2 - Immature seeds of soybean are becoming increasingly popular as a snack/vegetable to harness the health benefits of soybean. They are shelled from the immature pods picked from the mother plant at different reproductive stages. Information concerning changes in antioxidant constituents and antioxidant capacity during reproductive phases of soybean seeds is scarce. The aim of the present study was to determine whether immature seeds picked at different reproductive stages differ in tocopherol, isoflavone, total phenolic contents, free radical scavenging activity, and total antioxidative capacity. Seeds shelled from the soybean pods picked at three reproductive stages (R5, R6, and R7) as well as at full maturity were subjected to high-performance liquid chromatography analyses for tocopherol and isoflavone contents. Significantly higher values (P<0.05) were observed for tocopherols and isoflavones in immature seeds picked at late reproductive stages. At the first reproductive stage, that is, R5 stage, delta-tocopherol was the predominant form of tocopherol, whereas in subsequent reproductive stages as well as at complete maturity stage, the gamma-isomer contributed maximum proportion to the total tocopherol content. Genistein was, in general, the major form of isoflavone at all reproductive stages. Reduction in free radical scavenging activity, total antioxidant capacity, and total phenolic content in late-picked seeds concomitant with increased concentration of tocopherol and isoflavone isomers was observed. The results show that bioactive constituents other than isoflavones and tocopherols may decline with the advancement of maturity. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/19256542/Relative_changes_in_tocopherols_isoflavones_total_phenolic_content_and_antioxidative_activity_in_soybean_seeds_at_different_reproductive_stages_ L2 - https://doi.org/10.1021/jf803122a DB - PRIME DP - Unbound Medicine ER -