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[Suppression of murine EAE by triptolide is related to downregulation of INF-gamma and upregulation of IL-10 secretion in splenic lymphocytes].

Abstract

AIM

To explore the therapeutic effectivity and the possible mechanism of triptolide (Tri) on experimental autoimmune encephalomyelitis (EAE).

METHODS

All female C57BL/6 mice were randomly divided into EAE group (28), Tri treated group (20) and adjuvant group (18). Mice in EAE and treated groups were immunized with myelin oligodendrocyte glycoprotein peptides 35-55 (MOG(35-55);), adjuvant group was injected at the same time, but instead of MOG(35-55); with normal saline. Tri was intraperitoneally injected in the dosage of 100 microg/(kg.d) in treated group on day 5 post-immunization (p.i.), and mice in EAE and adjuvant group injected with normal saline as control. The clinical feature and pathological changes were observed and the splenic lymphocytes were prepared on days 18-20 p.i. The cell cultures were divided into the control group (only 200 microL of cell suspension) and the experimental group (cell suspension in the presence of 10 mg/L MOG(35-55);). Then all of them were inoculated in 96-well flat-bottom plates under 37 degrees Celsius, 50 mL/L CO(2);. After 48 h, the proliferation assay was determined by MTT, and the supernatants were harvested for the detection of INF-gamma, IL-17, IL-10 and IL-4 by ELISA.

RESULTS

Tri treatment showed an significantly protective action on EAE. After the intervention of Tri, the levels of IL-10 were increased (P<0.05), but the secretion of INF-gamma and proliferation response of splenic lymphocytes induced by MOG(35-55); were statistically significantly inhibited(P<0.05 and P<0.01, respectively). There were no influences on the amount of IL-17 and IL-4 by Tri.

CONCLUSION

Tri is an effective drug in suppressing murine EAE. This suppression is supposed to be related to downregulation of INF-gamma and upregulation of IL-10 secretion in splenic lymphocytes.

Authors+Show Affiliations

Institute of Brain Science, Shanxi Datong University, Datong, China. fhc_002@163.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

chi

PubMed ID

19257986

Citation

Fan, Hong-cui, et al. "[Suppression of Murine EAE By Triptolide Is Related to Downregulation of INF-gamma and Upregulation of IL-10 Secretion in Splenic Lymphocytes]." Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi = Chinese Journal of Cellular and Molecular Immunology, vol. 25, no. 3, 2009, pp. 222-5.
Fan HC, Ren XR, Yu JZ, et al. [Suppression of murine EAE by triptolide is related to downregulation of INF-gamma and upregulation of IL-10 secretion in splenic lymphocytes]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2009;25(3):222-5.
Fan, H. C., Ren, X. R., Yu, J. Z., Guo, M. F., Ji, N., Sun, Y. S., ... Ma, C. G. (2009). [Suppression of murine EAE by triptolide is related to downregulation of INF-gamma and upregulation of IL-10 secretion in splenic lymphocytes]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi = Chinese Journal of Cellular and Molecular Immunology, 25(3), pp. 222-5.
Fan HC, et al. [Suppression of Murine EAE By Triptolide Is Related to Downregulation of INF-gamma and Upregulation of IL-10 Secretion in Splenic Lymphocytes]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2009;25(3):222-5. PubMed PMID: 19257986.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Suppression of murine EAE by triptolide is related to downregulation of INF-gamma and upregulation of IL-10 secretion in splenic lymphocytes]. AU - Fan,Hong-cui, AU - Ren,Xiao-rong, AU - Yu,Jie-zhong, AU - Guo,Min-fang, AU - Ji,Ning, AU - Sun,Yong-sheng, AU - Liang,Li-yun, AU - Ma,Cun-gen, PY - 2009/3/5/entrez PY - 2009/3/5/pubmed PY - 2010/3/12/medline SP - 222 EP - 5 JF - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology JO - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi VL - 25 IS - 3 N2 - AIM: To explore the therapeutic effectivity and the possible mechanism of triptolide (Tri) on experimental autoimmune encephalomyelitis (EAE). METHODS: All female C57BL/6 mice were randomly divided into EAE group (28), Tri treated group (20) and adjuvant group (18). Mice in EAE and treated groups were immunized with myelin oligodendrocyte glycoprotein peptides 35-55 (MOG(35-55);), adjuvant group was injected at the same time, but instead of MOG(35-55); with normal saline. Tri was intraperitoneally injected in the dosage of 100 microg/(kg.d) in treated group on day 5 post-immunization (p.i.), and mice in EAE and adjuvant group injected with normal saline as control. The clinical feature and pathological changes were observed and the splenic lymphocytes were prepared on days 18-20 p.i. The cell cultures were divided into the control group (only 200 microL of cell suspension) and the experimental group (cell suspension in the presence of 10 mg/L MOG(35-55);). Then all of them were inoculated in 96-well flat-bottom plates under 37 degrees Celsius, 50 mL/L CO(2);. After 48 h, the proliferation assay was determined by MTT, and the supernatants were harvested for the detection of INF-gamma, IL-17, IL-10 and IL-4 by ELISA. RESULTS: Tri treatment showed an significantly protective action on EAE. After the intervention of Tri, the levels of IL-10 were increased (P<0.05), but the secretion of INF-gamma and proliferation response of splenic lymphocytes induced by MOG(35-55); were statistically significantly inhibited(P<0.05 and P<0.01, respectively). There were no influences on the amount of IL-17 and IL-4 by Tri. CONCLUSION: Tri is an effective drug in suppressing murine EAE. This suppression is supposed to be related to downregulation of INF-gamma and upregulation of IL-10 secretion in splenic lymphocytes. SN - 1007-8738 UR - https://www.unboundmedicine.com/medline/citation/19257986/[Suppression_of_murine_EAE_by_triptolide_is_related_to_downregulation_of_INF_gamma_and_upregulation_of_IL_10_secretion_in_splenic_lymphocytes]_ DB - PRIME DP - Unbound Medicine ER -