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[Application of flow microbeads assay in detection of specific antibodies and cytokines in serum from patients with hemorrhagic fever with renal syndrome].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2009 Mar; 25(3):245-7.XB

Abstract

AIM

To establish a flow microbeads assay (FMA) to examine the level of hemorrhagic fever with renal syndrome virus (HFRS V.) specific IgM, IgG and cytokines in HFRS patients.

METHODS

Serum samples from 28 cases of HFRS and 20 healthy controls were studied. Serum levels of anti-HFRS V. antibodies were qualified and inflammatory cytokines IL-6 and TNF-alpha were quantified by FMA. The results were compared with the results by ELISA.

RESULTS

FMA showed that the positivity rates for anti-HFRS V. IgM and IgG were 92.85% and 71.43%, respectively. None was detected positive in healthy control group. The serum level of IL-6 and TNF-alpha in HFRS group were (532.62+/-397.19) ng/L and (392.68+/-177.68)ng/L, respectively, which were significantly higher than that in healthy control group (38.77+/-20.32 ng/L and 15.91+/-6.91 ng/L, P<0.01). In contrast, ELISA showed that the positivity rates for anti-HFRS V. IgM and IgG were 71.43% and 50.00%, respectively. None was detected positive in healthy control group. The serum level of IL-6 and TNF-alpha in HFRS group were (256.46+/-102.51) ng/L ang (45.63+/-5.32) ng/L, respectively, which were significantly higher than that in healthy control group (53.80+/-19.21 ng/L and 5.81+/-3.58 ng/L, P<0.01).

CONCLUSION

A FMA method is established to monitor the immune response in HFRS patients, which has better sensitivity than ELISA, and thus provides a useful tool for study of HFRS.

Authors+Show Affiliations

Central Laboratory, Shaanxi Provincial People's Hospital, Xi'an, China. zhangjie810@yahoo.com.cnNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

19257990

Citation

Zhang, Jie, et al. "[Application of Flow Microbeads Assay in Detection of Specific Antibodies and Cytokines in Serum From Patients With Hemorrhagic Fever With Renal Syndrome]." Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi = Chinese Journal of Cellular and Molecular Immunology, vol. 25, no. 3, 2009, pp. 245-7.
Zhang J, Lin Y, Jin BQ. [Application of flow microbeads assay in detection of specific antibodies and cytokines in serum from patients with hemorrhagic fever with renal syndrome]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2009;25(3):245-7.
Zhang, J., Lin, Y., & Jin, B. Q. (2009). [Application of flow microbeads assay in detection of specific antibodies and cytokines in serum from patients with hemorrhagic fever with renal syndrome]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi = Chinese Journal of Cellular and Molecular Immunology, 25(3), 245-7.
Zhang J, Lin Y, Jin BQ. [Application of Flow Microbeads Assay in Detection of Specific Antibodies and Cytokines in Serum From Patients With Hemorrhagic Fever With Renal Syndrome]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2009;25(3):245-7. PubMed PMID: 19257990.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Application of flow microbeads assay in detection of specific antibodies and cytokines in serum from patients with hemorrhagic fever with renal syndrome]. AU - Zhang,Jie, AU - Lin,Yuan, AU - Jin,Bo-quan, PY - 2009/3/5/entrez PY - 2009/3/5/pubmed PY - 2010/3/12/medline SP - 245 EP - 7 JF - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology JO - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi VL - 25 IS - 3 N2 - AIM: To establish a flow microbeads assay (FMA) to examine the level of hemorrhagic fever with renal syndrome virus (HFRS V.) specific IgM, IgG and cytokines in HFRS patients. METHODS: Serum samples from 28 cases of HFRS and 20 healthy controls were studied. Serum levels of anti-HFRS V. antibodies were qualified and inflammatory cytokines IL-6 and TNF-alpha were quantified by FMA. The results were compared with the results by ELISA. RESULTS: FMA showed that the positivity rates for anti-HFRS V. IgM and IgG were 92.85% and 71.43%, respectively. None was detected positive in healthy control group. The serum level of IL-6 and TNF-alpha in HFRS group were (532.62+/-397.19) ng/L and (392.68+/-177.68)ng/L, respectively, which were significantly higher than that in healthy control group (38.77+/-20.32 ng/L and 15.91+/-6.91 ng/L, P<0.01). In contrast, ELISA showed that the positivity rates for anti-HFRS V. IgM and IgG were 71.43% and 50.00%, respectively. None was detected positive in healthy control group. The serum level of IL-6 and TNF-alpha in HFRS group were (256.46+/-102.51) ng/L ang (45.63+/-5.32) ng/L, respectively, which were significantly higher than that in healthy control group (53.80+/-19.21 ng/L and 5.81+/-3.58 ng/L, P<0.01). CONCLUSION: A FMA method is established to monitor the immune response in HFRS patients, which has better sensitivity than ELISA, and thus provides a useful tool for study of HFRS. SN - 1007-8738 UR - https://www.unboundmedicine.com/medline/citation/19257990/[Application_of_flow_microbeads_assay_in_detection_of_specific_antibodies_and_cytokines_in_serum_from_patients_with_hemorrhagic_fever_with_renal_syndrome]_ L2 - http://www.diseaseinfosearch.org/result/3317 DB - PRIME DP - Unbound Medicine ER -