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Highly specific detection of interleukin-6 (IL-6) protein using combination tapered fiber-optic biosensor dip-probe.
Biosens Bioelectron. 2009 Apr 15; 24(8):2696-701.BB

Abstract

We are reporting highly specific and sensitive detection of human interleukin-6 (IL-6) protein using combination tapered fiber-optic biosensor (CTFOB) dip-probe. With these probes we could successfully detect IL-6, down to a concentration of 5 pM (0.12 ng/ml) in the presence of much higher concentration of a non specific protein. Sandwich immunoassay was used to generate specific fluorescence signal. A novel strategy is developed to eliminate the false signal from non specific binding. In this new strategy it is not required to pre-treat probe surface with any kind of blocking buffer. The specificity of the sensor was established by incubating negative control probes in high concentration (1 nM) of another cytokine IL-8.

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Authors+Show Affiliations

Kapoor R
Department of Physics, University of Alabama at Birmingham, Birmingham, AL 35294, United States. rkapoor@uab.edu
Wang CW
No affiliation info available

MeSH

Biosensing TechniquesComputer-Aided DesignEquipment DesignEquipment Failure AnalysisFiber Optic TechnologyInterleukin-6Reproducibility of ResultsSensitivity and SpecificitySpectrum Analysis

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

19261461

Citation

Kapoor, Rakesh, and Chun-Wei Wang. "Highly Specific Detection of Interleukin-6 (IL-6) Protein Using Combination Tapered Fiber-optic Biosensor Dip-probe." Biosensors & Bioelectronics, vol. 24, no. 8, 2009, pp. 2696-701.
Kapoor R, Wang CW. Highly specific detection of interleukin-6 (IL-6) protein using combination tapered fiber-optic biosensor dip-probe. Biosens Bioelectron. 2009;24(8):2696-701.
Kapoor, R., & Wang, C. W. (2009). Highly specific detection of interleukin-6 (IL-6) protein using combination tapered fiber-optic biosensor dip-probe. Biosensors & Bioelectronics, 24(8), 2696-701. https://doi.org/10.1016/j.bios.2009.01.046
Kapoor R, Wang CW. Highly Specific Detection of Interleukin-6 (IL-6) Protein Using Combination Tapered Fiber-optic Biosensor Dip-probe. Biosens Bioelectron. 2009 Apr 15;24(8):2696-701. PubMed PMID: 19261461.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Highly specific detection of interleukin-6 (IL-6) protein using combination tapered fiber-optic biosensor dip-probe. AU - Kapoor,Rakesh, AU - Wang,Chun-Wei, Y1 - 2009/02/10/ PY - 2008/12/15/received PY - 2009/01/24/revised PY - 2009/01/30/accepted PY - 2009/3/6/entrez PY - 2009/3/6/pubmed PY - 2009/5/27/medline SP - 2696 EP - 701 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 24 IS - 8 N2 - We are reporting highly specific and sensitive detection of human interleukin-6 (IL-6) protein using combination tapered fiber-optic biosensor (CTFOB) dip-probe. With these probes we could successfully detect IL-6, down to a concentration of 5 pM (0.12 ng/ml) in the presence of much higher concentration of a non specific protein. Sandwich immunoassay was used to generate specific fluorescence signal. A novel strategy is developed to eliminate the false signal from non specific binding. In this new strategy it is not required to pre-treat probe surface with any kind of blocking buffer. The specificity of the sensor was established by incubating negative control probes in high concentration (1 nM) of another cytokine IL-8. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/19261461/Highly_specific_detection_of_interleukin_6__IL_6__protein_using_combination_tapered_fiber_optic_biosensor_dip_probe_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(09)00062-1 DB - PRIME DP - Unbound Medicine ER -