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Accumulation of sumoylated Rad52 in checkpoint mutants perturbed in DNA replication.
DNA Repair (Amst). 2009 Jun 04; 8(6):690-6.DR

Abstract

Checkpoints are cellular surveillance and signaling pathways that regulate responses to DNA damage and perturbations of DNA replication. Here we show that high levels of sumoylated Rad52 are present in the mec1 sml1 and rad53 sml1 checkpoint mutants exposed to DNA-damaging agents such as methyl methanesulfonate (MMS) or the DNA replication inhibitor hydroxyurea (HU). The kinase-defective mutant rad53-K227A also showed high levels of Rad52 sumoylation. Elevated levels of Rad52 sumoylation occur in checkpoint mutants proceeding S phase being exposed DNA-damaging agent. Interestingly, chromatin immunoprecipitation (ChIP) on chip analyses revealed non-canonical chromosomal localization of Rad52 in the HU-treated rad53-K227A cells arrested in early S phase: Rad52 localization at dormant and early DNA replication origins. However, such unusual localization was not dependent on the sumoylation of Rad52. In addition, we also found that Rad52 could be highly sumoylated in the absence of Rad51. Double mutation of RAD51 and RAD53 exhibited the similar levels of Rad52 sumoylation to RAD53 single mutation. The significance and regulation mechanism of Rad52 sumoylation by checkpoint pathways will be discussed.

Authors+Show Affiliations

Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6-3, Aramaki, Aoba-ku, Sendai 980-8578, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19261547

Citation

Ohuchi, Takashi, et al. "Accumulation of Sumoylated Rad52 in Checkpoint Mutants Perturbed in DNA Replication." DNA Repair, vol. 8, no. 6, 2009, pp. 690-6.
Ohuchi T, Seki M, Kugou K, et al. Accumulation of sumoylated Rad52 in checkpoint mutants perturbed in DNA replication. DNA Repair (Amst). 2009;8(6):690-6.
Ohuchi, T., Seki, M., Kugou, K., Tada, S., Ohta, K., & Enomoto, T. (2009). Accumulation of sumoylated Rad52 in checkpoint mutants perturbed in DNA replication. DNA Repair, 8(6), 690-6. https://doi.org/10.1016/j.dnarep.2009.01.018
Ohuchi T, et al. Accumulation of Sumoylated Rad52 in Checkpoint Mutants Perturbed in DNA Replication. DNA Repair (Amst). 2009 Jun 4;8(6):690-6. PubMed PMID: 19261547.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Accumulation of sumoylated Rad52 in checkpoint mutants perturbed in DNA replication. AU - Ohuchi,Takashi, AU - Seki,Masayuki, AU - Kugou,Kazuto, AU - Tada,Shusuke, AU - Ohta,Kunihiro, AU - Enomoto,Takemi, Y1 - 2009/03/03/ PY - 2008/12/02/received PY - 2009/01/21/revised PY - 2009/01/26/accepted PY - 2009/3/6/entrez PY - 2009/3/6/pubmed PY - 2009/8/7/medline SP - 690 EP - 6 JF - DNA repair JO - DNA Repair (Amst) VL - 8 IS - 6 N2 - Checkpoints are cellular surveillance and signaling pathways that regulate responses to DNA damage and perturbations of DNA replication. Here we show that high levels of sumoylated Rad52 are present in the mec1 sml1 and rad53 sml1 checkpoint mutants exposed to DNA-damaging agents such as methyl methanesulfonate (MMS) or the DNA replication inhibitor hydroxyurea (HU). The kinase-defective mutant rad53-K227A also showed high levels of Rad52 sumoylation. Elevated levels of Rad52 sumoylation occur in checkpoint mutants proceeding S phase being exposed DNA-damaging agent. Interestingly, chromatin immunoprecipitation (ChIP) on chip analyses revealed non-canonical chromosomal localization of Rad52 in the HU-treated rad53-K227A cells arrested in early S phase: Rad52 localization at dormant and early DNA replication origins. However, such unusual localization was not dependent on the sumoylation of Rad52. In addition, we also found that Rad52 could be highly sumoylated in the absence of Rad51. Double mutation of RAD51 and RAD53 exhibited the similar levels of Rad52 sumoylation to RAD53 single mutation. The significance and regulation mechanism of Rad52 sumoylation by checkpoint pathways will be discussed. SN - 1568-7864 UR - https://www.unboundmedicine.com/medline/citation/19261547/Accumulation_of_sumoylated_Rad52_in_checkpoint_mutants_perturbed_in_DNA_replication_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1568-7864(09)00042-1 DB - PRIME DP - Unbound Medicine ER -