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Regulation of rDNA stability by sumoylation.
DNA Repair (Amst). 2009 Apr 05; 8(4):507-16.DR

Abstract

Repair of DNA lesions by homologous recombination relies on the copying of genetic information from an intact homologous sequence. However, many eukaryotic genomes contain repetitive sequences such as the ribosomal gene locus (rDNA), which poses a risk for illegitimate recombination. Therefore, the eukaryotic cell has evolved mechanisms to favor equal sister chromatid exchange (SCE) and suppress unequal SCE, single-strand annealing and break-induced replication. In the budding yeast Saccharomyces cerevisiae, the tight regulation of homologous recombination at the rDNA locus is dependent on the Smc5-Smc6 complex and sumoylation of Rad52, which directs DNA double-strand breaks in the rDNA to relocalize from within the nucleolus to the nucleoplasm before association with the recombination machinery. The relocalization before repair is important for maintaining rDNA stability. The focus of this review is the regulation of recombinational DNA repair at the rDNA locus by sumoylation and the Smc5-Smc6 complex in S. cerevisiae.

Authors+Show Affiliations

Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, DK-2200 Copenhagen N, Denmark.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

19261548

Citation

Eckert-Boulet, Nadine, and Michael Lisby. "Regulation of rDNA Stability By Sumoylation." DNA Repair, vol. 8, no. 4, 2009, pp. 507-16.
Eckert-Boulet N, Lisby M. Regulation of rDNA stability by sumoylation. DNA Repair (Amst). 2009;8(4):507-16.
Eckert-Boulet, N., & Lisby, M. (2009). Regulation of rDNA stability by sumoylation. DNA Repair, 8(4), 507-16. https://doi.org/10.1016/j.dnarep.2009.01.015
Eckert-Boulet N, Lisby M. Regulation of rDNA Stability By Sumoylation. DNA Repair (Amst). 2009 Apr 5;8(4):507-16. PubMed PMID: 19261548.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation of rDNA stability by sumoylation. AU - Eckert-Boulet,Nadine, AU - Lisby,Michael, Y1 - 2009/03/03/ PY - 2009/3/6/entrez PY - 2009/3/6/pubmed PY - 2009/6/25/medline SP - 507 EP - 16 JF - DNA repair JO - DNA Repair (Amst) VL - 8 IS - 4 N2 - Repair of DNA lesions by homologous recombination relies on the copying of genetic information from an intact homologous sequence. However, many eukaryotic genomes contain repetitive sequences such as the ribosomal gene locus (rDNA), which poses a risk for illegitimate recombination. Therefore, the eukaryotic cell has evolved mechanisms to favor equal sister chromatid exchange (SCE) and suppress unequal SCE, single-strand annealing and break-induced replication. In the budding yeast Saccharomyces cerevisiae, the tight regulation of homologous recombination at the rDNA locus is dependent on the Smc5-Smc6 complex and sumoylation of Rad52, which directs DNA double-strand breaks in the rDNA to relocalize from within the nucleolus to the nucleoplasm before association with the recombination machinery. The relocalization before repair is important for maintaining rDNA stability. The focus of this review is the regulation of recombinational DNA repair at the rDNA locus by sumoylation and the Smc5-Smc6 complex in S. cerevisiae. SN - 1568-7864 UR - https://www.unboundmedicine.com/medline/citation/19261548/Regulation_of_rDNA_stability_by_sumoylation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1568-7864(09)00016-0 DB - PRIME DP - Unbound Medicine ER -