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Antiviral activity of carbohydrate-binding agents and the role of DC-SIGN in dengue virus infection.
Virology. 2009 Apr 25; 387(1):67-75.V

Abstract

Dendritic cell-specific intercellular adhesion molecule 3-grabbing non-integrin (DC-SIGN) is an important binding receptor for dengue virus (DENV) that recognizes N-glycosylation sites on the viral E-glycoprotein. DENV cannot bind nor infect the human B-cell line Raji/0. However, DENV productively infects Raji/DC-SIGN(+) cells that constitutively express DC-SIGN on their surface. IL-4-treated monocytes, expressing high levels of DC-SIGN, are also susceptible for DENV infection. Several carbohydrate-binding agents (CBAs), such as the plant lectins HHA, GNA (mannose-specific) and UDA (N-acetylglucosamine-specific), inhibited dose-dependently the binding of DENV and subsequently viral replication in Raji/DC-SIGN(+) cells (EC(50): 0.1-2.2 microM). These CBAs were clearly more active against DENV in IL-4-treated monocytes (EC(50): 4-56 nM). However, the CBAs were devoid of antiviral activity in DENV-susceptible Vero-B (DC-SIGN(-)) cells, demonstrating cell type-dependent differences in viral entry mechanisms.

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Authors+Show Affiliations

Alen MM
Laboratory of Virology and Chemotherapy, Rega Institute for Medical Research, Katholieke Universiteit Leuven, Minderbroedersstraat 10, B-3000 Leuven, Belgium.
Kaptein SJ
No affiliation info available
De Burghgraeve T
No affiliation info available
Balzarini J
No affiliation info available
Neyts J
No affiliation info available
Schols D
No affiliation info available

MeSH

AnimalsAntiviral AgentsCell Adhesion MoleculesChlorocebus aethiopsDengueDengue VirusHumansInterleukin-4Lectins, C-TypeLeukocytes, MononuclearPlant LectinsReceptors, Cell SurfaceVero Cells

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19264337

Citation

Alen, Marijke M F., et al. "Antiviral Activity of Carbohydrate-binding Agents and the Role of DC-SIGN in Dengue Virus Infection." Virology, vol. 387, no. 1, 2009, pp. 67-75.
Alen MM, Kaptein SJ, De Burghgraeve T, et al. Antiviral activity of carbohydrate-binding agents and the role of DC-SIGN in dengue virus infection. Virology. 2009;387(1):67-75.
Alen, M. M., Kaptein, S. J., De Burghgraeve, T., Balzarini, J., Neyts, J., & Schols, D. (2009). Antiviral activity of carbohydrate-binding agents and the role of DC-SIGN in dengue virus infection. Virology, 387(1), 67-75. https://doi.org/10.1016/j.virol.2009.01.043
Alen MM, et al. Antiviral Activity of Carbohydrate-binding Agents and the Role of DC-SIGN in Dengue Virus Infection. Virology. 2009 Apr 25;387(1):67-75. PubMed PMID: 19264337.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antiviral activity of carbohydrate-binding agents and the role of DC-SIGN in dengue virus infection. AU - Alen,Marijke M F, AU - Kaptein,Suzanne J F, AU - De Burghgraeve,Tine, AU - Balzarini,Jan, AU - Neyts,Johan, AU - Schols,Dominique, Y1 - 2009/03/04/ PY - 2008/10/06/received PY - 2008/11/17/revised PY - 2009/01/26/accepted PY - 2009/3/7/entrez PY - 2009/3/7/pubmed PY - 2009/6/20/medline SP - 67 EP - 75 JF - Virology JO - Virology VL - 387 IS - 1 N2 - Dendritic cell-specific intercellular adhesion molecule 3-grabbing non-integrin (DC-SIGN) is an important binding receptor for dengue virus (DENV) that recognizes N-glycosylation sites on the viral E-glycoprotein. DENV cannot bind nor infect the human B-cell line Raji/0. However, DENV productively infects Raji/DC-SIGN(+) cells that constitutively express DC-SIGN on their surface. IL-4-treated monocytes, expressing high levels of DC-SIGN, are also susceptible for DENV infection. Several carbohydrate-binding agents (CBAs), such as the plant lectins HHA, GNA (mannose-specific) and UDA (N-acetylglucosamine-specific), inhibited dose-dependently the binding of DENV and subsequently viral replication in Raji/DC-SIGN(+) cells (EC(50): 0.1-2.2 microM). These CBAs were clearly more active against DENV in IL-4-treated monocytes (EC(50): 4-56 nM). However, the CBAs were devoid of antiviral activity in DENV-susceptible Vero-B (DC-SIGN(-)) cells, demonstrating cell type-dependent differences in viral entry mechanisms. SN - 1096-0341 UR - https://www.unboundmedicine.com/medline/citation/19264337/Antiviral_activity_of_carbohydrate_binding_agents_and_the_role_of_DC_SIGN_in_dengue_virus_infection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0042-6822(09)00076-2 DB - PRIME DP - Unbound Medicine ER -