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The Epstein-Barr virus lytic cycle activator Zta interacts with methylated ZRE in the promoter of host target gene egr1.
J Gen Virol. 2009 Jun; 90(Pt 6):1450-4.JG

Abstract

Activation of the host gene egr1 is essential for the lytic replication of Epstein-Barr virus (EBV). egr1 is activated by Zta (BZLF1, ZEBRA). Zta interacts directly with DNA through a series of closely related Zta-response elements (ZREs). Here we dissect the mechanism used by Zta to interact with the egr1 promoter and identify a weak interaction with egr1ZRE that is dependent on the distal part of egr1ZRE. Furthermore, we demonstrate that the ability of Zta to interact with egr1ZRE is enhanced at least tenfold by methylation. The ability of Zta to transactivate a reporter construct driven by the egr1 promoter can be enhanced by methylation. As the ability of Zta to interact with a methylated ZRE in the EBV genome correlates with its ability to activate the expression of the endogenous viral gene BRLF1, this suggests that Zta may also have the capability to overturn epigenetic control of egr1.

Authors+Show Affiliations

School of Life Sciences, University of Sussex, Brighton BN1 9QG, UK.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19264650

Citation

Heather, James, et al. "The Epstein-Barr Virus Lytic Cycle Activator Zta Interacts With Methylated ZRE in the Promoter of Host Target Gene Egr1." The Journal of General Virology, vol. 90, no. Pt 6, 2009, pp. 1450-4.
Heather J, Flower K, Isaac S, et al. The Epstein-Barr virus lytic cycle activator Zta interacts with methylated ZRE in the promoter of host target gene egr1. J Gen Virol. 2009;90(Pt 6):1450-4.
Heather, J., Flower, K., Isaac, S., & Sinclair, A. J. (2009). The Epstein-Barr virus lytic cycle activator Zta interacts with methylated ZRE in the promoter of host target gene egr1. The Journal of General Virology, 90(Pt 6), 1450-4. https://doi.org/10.1099/vir.0.007922-0
Heather J, et al. The Epstein-Barr Virus Lytic Cycle Activator Zta Interacts With Methylated ZRE in the Promoter of Host Target Gene Egr1. J Gen Virol. 2009;90(Pt 6):1450-4. PubMed PMID: 19264650.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Epstein-Barr virus lytic cycle activator Zta interacts with methylated ZRE in the promoter of host target gene egr1. AU - Heather,James, AU - Flower,Kirsty, AU - Isaac,Samine, AU - Sinclair,Alison J, Y1 - 2009/03/04/ PY - 2009/3/7/entrez PY - 2009/3/7/pubmed PY - 2009/6/26/medline SP - 1450 EP - 4 JF - The Journal of general virology JO - J. Gen. Virol. VL - 90 IS - Pt 6 N2 - Activation of the host gene egr1 is essential for the lytic replication of Epstein-Barr virus (EBV). egr1 is activated by Zta (BZLF1, ZEBRA). Zta interacts directly with DNA through a series of closely related Zta-response elements (ZREs). Here we dissect the mechanism used by Zta to interact with the egr1 promoter and identify a weak interaction with egr1ZRE that is dependent on the distal part of egr1ZRE. Furthermore, we demonstrate that the ability of Zta to interact with egr1ZRE is enhanced at least tenfold by methylation. The ability of Zta to transactivate a reporter construct driven by the egr1 promoter can be enhanced by methylation. As the ability of Zta to interact with a methylated ZRE in the EBV genome correlates with its ability to activate the expression of the endogenous viral gene BRLF1, this suggests that Zta may also have the capability to overturn epigenetic control of egr1. SN - 0022-1317 UR - https://www.unboundmedicine.com/medline/citation/19264650/The_Epstein_Barr_virus_lytic_cycle_activator_Zta_interacts_with_methylated_ZRE_in_the_promoter_of_host_target_gene_egr1_ L2 - http://jgv.microbiologyresearch.org/pubmed/content/journal/jgv/10.1099/vir.0.007922-0 DB - PRIME DP - Unbound Medicine ER -