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Enhancing production of L-serine by increasing the glyA gene expression in Methylobacterium sp. MB200.
Appl Biochem Biotechnol. 2010 Mar; 160(3):740-50.AB

Abstract

Microbial fermentation using methylotrophic bacteria is one of the most promising methods for L-serine production. Here we describe the metabolic engineering of a Methylobacterium strain to increase the production of L-serine. The glyA gene, encoding serine hydroxymethyltransferase (SHMT), was isolated from the genomic DNA of Methylobacterium sp. MB200, using a DNA fragment encoding Methylobacterium extorquens AM1 SHMT as a probe, and inserted into the vector pLAFR3. The resulting construct was transformed into Methylobacterium sp. MB200 using triparental mating. The genetic-engineered strain, designated as Methylobacterium sp. MB202, was shown to produce 11.4 + or - 0.6 mg/ml serine in resting cell reactions from 30 mg/ml wet cells, 20 mg/ml glycine, and 70 mg/ml methanol in 2 days, representing a 4.4-fold increase from that of the wild strain. The results demonstrated the potential for improving L-serine production by manipulating the glyA in bacteria and should facilitate the production of L-serine using Methylobacterium sp. strains.

Authors+Show Affiliations

College of Life Science and Technology, The Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530005, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19266321

Citation

Shen, Peihong, et al. "Enhancing Production of L-serine By Increasing the glyA Gene Expression in Methylobacterium Sp. MB200." Applied Biochemistry and Biotechnology, vol. 160, no. 3, 2010, pp. 740-50.
Shen P, Chao H, Jiang C, et al. Enhancing production of L-serine by increasing the glyA gene expression in Methylobacterium sp. MB200. Appl Biochem Biotechnol. 2010;160(3):740-50.
Shen, P., Chao, H., Jiang, C., Long, Z., Wang, C., & Wu, B. (2010). Enhancing production of L-serine by increasing the glyA gene expression in Methylobacterium sp. MB200. Applied Biochemistry and Biotechnology, 160(3), 740-50. https://doi.org/10.1007/s12010-009-8551-x
Shen P, et al. Enhancing Production of L-serine By Increasing the glyA Gene Expression in Methylobacterium Sp. MB200. Appl Biochem Biotechnol. 2010;160(3):740-50. PubMed PMID: 19266321.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enhancing production of L-serine by increasing the glyA gene expression in Methylobacterium sp. MB200. AU - Shen,Peihong, AU - Chao,Hongjun, AU - Jiang,Chengjian, AU - Long,Zhangde, AU - Wang,Changhao, AU - Wu,Bo, Y1 - 2009/03/06/ PY - 2008/10/20/received PY - 2009/01/29/accepted PY - 2009/3/7/entrez PY - 2009/3/7/pubmed PY - 2010/3/24/medline SP - 740 EP - 50 JF - Applied biochemistry and biotechnology JO - Appl. Biochem. Biotechnol. VL - 160 IS - 3 N2 - Microbial fermentation using methylotrophic bacteria is one of the most promising methods for L-serine production. Here we describe the metabolic engineering of a Methylobacterium strain to increase the production of L-serine. The glyA gene, encoding serine hydroxymethyltransferase (SHMT), was isolated from the genomic DNA of Methylobacterium sp. MB200, using a DNA fragment encoding Methylobacterium extorquens AM1 SHMT as a probe, and inserted into the vector pLAFR3. The resulting construct was transformed into Methylobacterium sp. MB200 using triparental mating. The genetic-engineered strain, designated as Methylobacterium sp. MB202, was shown to produce 11.4 + or - 0.6 mg/ml serine in resting cell reactions from 30 mg/ml wet cells, 20 mg/ml glycine, and 70 mg/ml methanol in 2 days, representing a 4.4-fold increase from that of the wild strain. The results demonstrated the potential for improving L-serine production by manipulating the glyA in bacteria and should facilitate the production of L-serine using Methylobacterium sp. strains. SN - 1559-0291 UR - https://www.unboundmedicine.com/medline/citation/19266321/Enhancing_production_of_L_serine_by_increasing_the_glyA_gene_expression_in_Methylobacterium_sp__MB200_ L2 - https://dx.doi.org/10.1007/s12010-009-8551-x DB - PRIME DP - Unbound Medicine ER -