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Suppression of reactive oxygen species and nitric oxide by Asparagus racemosus root extract using in vitro studies.
Cell Mol Biol (Noisy-le-grand). 2009 Feb 25; 55 Suppl:OL1083-95.CM

Abstract

Recent clinical and experimental data showed the involvement of reactive oxygen species/nitrogen species (ROS/RNS) in many human pathophysiological conditions. Antioxidant activity of the aqueous (ARA) and ethanolic extracts (ARE) of Asparagus racemosus (AR) root were evaluated in a series of in vitro assays including ROS generation in chemicals and biological model systems. The dose-dependent ARA and ARE extracts showed the scavenging activity against DPPH (IC50 = 60.7 and 52.5 microg/ml), nitric oxide (IC50 = 141.9 and 63.4 microg/ml), superoxide (IC50 = 221 and 89.4 microg/ml), hydroxyl (IC50 = 318.7 and 208.8 microg/ml) and ABTS.+ (IC50 = 134.5 and 71.9 microg/ml) radicals. The antioxidant capacity of ARA and ARE were assessed for their reducing power using FRAP (Ferric Reducing antioxidant power) and potassium ferricyanide reducing methods as well as free radical scavenging capacity by TEAC (Trolox Equivalent Antioxidant Capacity) method. ARA and ARE extracts were also found to be effective at suppressing lipid peroxidation induced by Fe2+/ascorbate system in rat liver mitochondrial preparation (IC50 = 511.7 and 309.2 microg/ml, respectively). Further, ARA and ARE root extracts significantly decreased (P < 0.05) copper-mediated human LDL oxidation by prolongation of lag phase time with decline in oxidation rate, maximal yield of conjugated dienes, lipid hydroperoxides and malondialdehyde concentrations. The addition of ARA and ARE root extracts to human serum significantly reduced (P < 0.05) the formation of lipid peroxidation in medium. Trolox, alpha-tocopherol and mannitol were tested similarly to compare their antioxidant activities. In conclusion, antioxidant activity of ARE as compared to ARA extract is more effective which act as hydrogen donors, metal ion chelators, reducing agents, radical scavengers and anti-lipid peroxidative. These effects are attributed to the high amount of lipophilic phenolics content of ARE root extract.

Authors+Show Affiliations

BRD School of Biosciences, Sardar Patel Maidan, Vadtal Road, Satellite Campus, Postbox 39, Sardar Patel University, Vallabh Vidyanagar 388 120, Gujarat, India.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19267991

Citation

Visavadiya, N P., et al. "Suppression of Reactive Oxygen Species and Nitric Oxide By Asparagus Racemosus Root Extract Using in Vitro Studies." Cellular and Molecular Biology (Noisy-le-Grand, France), vol. 55 Suppl, 2009, pp. OL1083-95.
Visavadiya NP, Soni B, Soni B, et al. Suppression of reactive oxygen species and nitric oxide by Asparagus racemosus root extract using in vitro studies. Cell Mol Biol (Noisy-le-grand). 2009;55 Suppl:OL1083-95.
Visavadiya, N. P., Soni, B., Soni, B., & Madamwar, D. (2009). Suppression of reactive oxygen species and nitric oxide by Asparagus racemosus root extract using in vitro studies. Cellular and Molecular Biology (Noisy-le-Grand, France), 55 Suppl, OL1083-95.
Visavadiya NP, et al. Suppression of Reactive Oxygen Species and Nitric Oxide By Asparagus Racemosus Root Extract Using in Vitro Studies. Cell Mol Biol (Noisy-le-grand). 2009 Feb 25;55 Suppl:OL1083-95. PubMed PMID: 19267991.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Suppression of reactive oxygen species and nitric oxide by Asparagus racemosus root extract using in vitro studies. AU - Visavadiya,N P, AU - Soni,B, AU - Soni,B, AU - Madamwar,D, Y1 - 2009/02/25/ PY - 2008/09/01/received PY - 2008/12/29/accepted PY - 2009/3/10/entrez PY - 2009/3/17/pubmed PY - 2009/5/21/medline SP - OL1083 EP - 95 JF - Cellular and molecular biology (Noisy-le-Grand, France) JO - Cell Mol Biol (Noisy-le-grand) VL - 55 Suppl N2 - Recent clinical and experimental data showed the involvement of reactive oxygen species/nitrogen species (ROS/RNS) in many human pathophysiological conditions. Antioxidant activity of the aqueous (ARA) and ethanolic extracts (ARE) of Asparagus racemosus (AR) root were evaluated in a series of in vitro assays including ROS generation in chemicals and biological model systems. The dose-dependent ARA and ARE extracts showed the scavenging activity against DPPH (IC50 = 60.7 and 52.5 microg/ml), nitric oxide (IC50 = 141.9 and 63.4 microg/ml), superoxide (IC50 = 221 and 89.4 microg/ml), hydroxyl (IC50 = 318.7 and 208.8 microg/ml) and ABTS.+ (IC50 = 134.5 and 71.9 microg/ml) radicals. The antioxidant capacity of ARA and ARE were assessed for their reducing power using FRAP (Ferric Reducing antioxidant power) and potassium ferricyanide reducing methods as well as free radical scavenging capacity by TEAC (Trolox Equivalent Antioxidant Capacity) method. ARA and ARE extracts were also found to be effective at suppressing lipid peroxidation induced by Fe2+/ascorbate system in rat liver mitochondrial preparation (IC50 = 511.7 and 309.2 microg/ml, respectively). Further, ARA and ARE root extracts significantly decreased (P < 0.05) copper-mediated human LDL oxidation by prolongation of lag phase time with decline in oxidation rate, maximal yield of conjugated dienes, lipid hydroperoxides and malondialdehyde concentrations. The addition of ARA and ARE root extracts to human serum significantly reduced (P < 0.05) the formation of lipid peroxidation in medium. Trolox, alpha-tocopherol and mannitol were tested similarly to compare their antioxidant activities. In conclusion, antioxidant activity of ARE as compared to ARA extract is more effective which act as hydrogen donors, metal ion chelators, reducing agents, radical scavengers and anti-lipid peroxidative. These effects are attributed to the high amount of lipophilic phenolics content of ARE root extract. SN - 1165-158X UR - https://www.unboundmedicine.com/medline/citation/19267991/Suppression_of_reactive_oxygen_species_and_nitric_oxide_by_Asparagus_racemosus_root_extract_using_in_vitro_studies_ L2 - https://antibodies.cancer.gov/detail/CPTC-MTOR-8 DB - PRIME DP - Unbound Medicine ER -