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A lipoxygenase with dual positional specificity is expressed in olives (Olea europaea L.) during ripening.
Biochim Biophys Acta. 2009 May; 1791(5):339-46.BB

Abstract

Plant lipoxygenases (LOXs) are a class of widespread dioxygenases catalysing the hydroperoxidation of polyunsaturated fatty acids. Although multiple isoforms of LOX have been detected in a wide range of plants, their physiological roles remain to be clarified. With the aim to clarify the occurrence of LOXs in olives and their contribution to the elaboration of the olive oil aroma, we cloned and characterized the first cDNA of the LOX isoform which is expressed during olive development. The open reading frame encodes a polypeptide of 864 amino acids. This olive LOX is a type-1 LOX which shows a high degree of identity at the peptide level towards hazelnut (77.3%), tobacco (76.3%) and almond (75.5%) LOXs. The recombinant enzyme shows a dual positional specificity, as it forms both 9- and 13-hydroperoxide of linoleic acid in a 2:1 ratio, and would be defined as 9/13-LOX. Although a LOX activity was detected throughout the olive development, the 9/13-LOX is mainly expressed at late developmental stages. Our data suggest that there are at least two Lox genes expressed in black olives, and that the 9/13-LOX is associated with the ripening and senescence processes. However, due to its dual positional specificity and its expression pattern, its contribution to the elaboration of the olive oil aroma might be considered.

Authors+Show Affiliations

Université de Corse, CNRS UMR6134 SPE, Laboratoire de Biochimie et Biologie Moléculaire Végétales, campus Grimaldi, BP52, 20250 Corte, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19268561

Citation

Palmieri-Thiers, Cynthia, et al. "A Lipoxygenase With Dual Positional Specificity Is Expressed in Olives (Olea Europaea L.) During Ripening." Biochimica Et Biophysica Acta, vol. 1791, no. 5, 2009, pp. 339-46.
Palmieri-Thiers C, Canaan S, Brunini V, et al. A lipoxygenase with dual positional specificity is expressed in olives (Olea europaea L.) during ripening. Biochim Biophys Acta. 2009;1791(5):339-46.
Palmieri-Thiers, C., Canaan, S., Brunini, V., Lorenzi, V., Tomi, F., Desseyn, J. L., Garscha, U., Oliw, E. H., Berti, L., & Maury, J. (2009). A lipoxygenase with dual positional specificity is expressed in olives (Olea europaea L.) during ripening. Biochimica Et Biophysica Acta, 1791(5), 339-46. https://doi.org/10.1016/j.bbalip.2009.02.012
Palmieri-Thiers C, et al. A Lipoxygenase With Dual Positional Specificity Is Expressed in Olives (Olea Europaea L.) During Ripening. Biochim Biophys Acta. 2009;1791(5):339-46. PubMed PMID: 19268561.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A lipoxygenase with dual positional specificity is expressed in olives (Olea europaea L.) during ripening. AU - Palmieri-Thiers,Cynthia, AU - Canaan,Stéphane, AU - Brunini,Virginie, AU - Lorenzi,Vannina, AU - Tomi,Félix, AU - Desseyn,Jean-Luc, AU - Garscha,Ulrike, AU - Oliw,Ernst H, AU - Berti,Liliane, AU - Maury,Jacques, PY - 2008/07/21/received PY - 2009/01/09/revised PY - 2009/02/12/accepted PY - 2009/3/10/entrez PY - 2009/3/10/pubmed PY - 2009/12/16/medline SP - 339 EP - 46 JF - Biochimica et biophysica acta JO - Biochim Biophys Acta VL - 1791 IS - 5 N2 - Plant lipoxygenases (LOXs) are a class of widespread dioxygenases catalysing the hydroperoxidation of polyunsaturated fatty acids. Although multiple isoforms of LOX have been detected in a wide range of plants, their physiological roles remain to be clarified. With the aim to clarify the occurrence of LOXs in olives and their contribution to the elaboration of the olive oil aroma, we cloned and characterized the first cDNA of the LOX isoform which is expressed during olive development. The open reading frame encodes a polypeptide of 864 amino acids. This olive LOX is a type-1 LOX which shows a high degree of identity at the peptide level towards hazelnut (77.3%), tobacco (76.3%) and almond (75.5%) LOXs. The recombinant enzyme shows a dual positional specificity, as it forms both 9- and 13-hydroperoxide of linoleic acid in a 2:1 ratio, and would be defined as 9/13-LOX. Although a LOX activity was detected throughout the olive development, the 9/13-LOX is mainly expressed at late developmental stages. Our data suggest that there are at least two Lox genes expressed in black olives, and that the 9/13-LOX is associated with the ripening and senescence processes. However, due to its dual positional specificity and its expression pattern, its contribution to the elaboration of the olive oil aroma might be considered. SN - 0006-3002 UR - https://www.unboundmedicine.com/medline/citation/19268561/A_lipoxygenase_with_dual_positional_specificity_is_expressed_in_olives__Olea_europaea_L___during_ripening_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1388-1981(09)00060-2 DB - PRIME DP - Unbound Medicine ER -