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Separation of amino acids and amines by capillary electrophoresis using poly(ethylene oxide) solution containing cetyltrimethylammonium bromide.
J Chromatogr A. 2009 Oct 30; 1216(44):7576-81.JC

Abstract

We describe simultaneous analysis of naphthalene-2,3-dicarboxaldehyde (NDA)-amino acid and amine derivatives by capillary electrophoresis in conjunction with light-emitting diode-induced fluorescence (LEDIF) detection using poly(ethylene oxide) (PEO) containing cetyltrimethylammonium bromide (CTAB). In the presence of CTAB and acetonitrile (ACN), adsorption of PEO on the capillary wall is suppressed, leading to generation of a fast and reproducible electroosmotic flow (EOF). In order to optimize separation resolution and speed, 100 mM Tris-borate solution (pH 7.0) containing 20 mM CTAB and 25% ACN was used to fill the capillary and to prepare 1.2% PEO that entered the capillary via EOF. The analysis of 14 NDA-amino acid and -amine derivatives by this approach is rapid (<4 min), efficient ((0.9-6.4) x 10(5) theoretical plates), and sensitive (the LODs (S/N=3) range from 9.5 to 50.5 nM). The RSD values (n=5) of the migration times and peak heights of the analytes for the intraday analysis are less than 1.5 and 1.2%, respectively. We have validated the practicality of this approach by quantitative determination of 10 amino acids and amines in a beer samples within 4 min.

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Authors+Show Affiliations

Chen CK
Department of Chemistry, National Taiwan University, Taipei, Taiwan.
Liu KT
No affiliation info available
Chiu TC
No affiliation info available
Chang HT
No affiliation info available

MeSH

AminesAmino AcidsBeerCetrimoniumCetrimonium CompoundsElectrophoresis, CapillaryPolyethylene Glycols

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19268952

Citation

Chen, Chuan-Kuo, et al. "Separation of Amino Acids and Amines By Capillary Electrophoresis Using Poly(ethylene Oxide) Solution Containing Cetyltrimethylammonium Bromide." Journal of Chromatography. A, vol. 1216, no. 44, 2009, pp. 7576-81.
Chen CK, Liu KT, Chiu TC, et al. Separation of amino acids and amines by capillary electrophoresis using poly(ethylene oxide) solution containing cetyltrimethylammonium bromide. J Chromatogr A. 2009;1216(44):7576-81.
Chen, C. K., Liu, K. T., Chiu, T. C., & Chang, H. T. (2009). Separation of amino acids and amines by capillary electrophoresis using poly(ethylene oxide) solution containing cetyltrimethylammonium bromide. Journal of Chromatography. A, 1216(44), 7576-81. https://doi.org/10.1016/j.chroma.2009.02.054
Chen CK, et al. Separation of Amino Acids and Amines By Capillary Electrophoresis Using Poly(ethylene Oxide) Solution Containing Cetyltrimethylammonium Bromide. J Chromatogr A. 2009 Oct 30;1216(44):7576-81. PubMed PMID: 19268952.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Separation of amino acids and amines by capillary electrophoresis using poly(ethylene oxide) solution containing cetyltrimethylammonium bromide. AU - Chen,Chuan-Kuo, AU - Liu,Kung-Tien, AU - Chiu,Tai-Chia, AU - Chang,Huan-Tsung, Y1 - 2009/02/23/ PY - 2008/12/24/received PY - 2009/02/13/revised PY - 2009/02/16/accepted PY - 2009/3/10/entrez PY - 2009/3/10/pubmed PY - 2009/12/23/medline SP - 7576 EP - 81 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1216 IS - 44 N2 - We describe simultaneous analysis of naphthalene-2,3-dicarboxaldehyde (NDA)-amino acid and amine derivatives by capillary electrophoresis in conjunction with light-emitting diode-induced fluorescence (LEDIF) detection using poly(ethylene oxide) (PEO) containing cetyltrimethylammonium bromide (CTAB). In the presence of CTAB and acetonitrile (ACN), adsorption of PEO on the capillary wall is suppressed, leading to generation of a fast and reproducible electroosmotic flow (EOF). In order to optimize separation resolution and speed, 100 mM Tris-borate solution (pH 7.0) containing 20 mM CTAB and 25% ACN was used to fill the capillary and to prepare 1.2% PEO that entered the capillary via EOF. The analysis of 14 NDA-amino acid and -amine derivatives by this approach is rapid (<4 min), efficient ((0.9-6.4) x 10(5) theoretical plates), and sensitive (the LODs (S/N=3) range from 9.5 to 50.5 nM). The RSD values (n=5) of the migration times and peak heights of the analytes for the intraday analysis are less than 1.5 and 1.2%, respectively. We have validated the practicality of this approach by quantitative determination of 10 amino acids and amines in a beer samples within 4 min. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/19268952/Separation_of_amino_acids_and_amines_by_capillary_electrophoresis_using_poly_ethylene_oxide__solution_containing_cetyltrimethylammonium_bromide_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(09)00304-5 DB - PRIME DP - Unbound Medicine ER -