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Regulation of cellular glutathione peroxidase by different forms and concentrations of selenium in primary cultured bovine hepatocytes.
J Nutr Biochem. 2010 Feb; 21(2):153-61.JN

Abstract

The expression and activity of cellular glutathione peroxidase (GPx1) are regulated by selenium (Se). Generally speaking, organic forms of Se have less toxicity and greater bioavailability compared with inorganic forms. In this study, the effects of different forms and concentrations of Se on the regulation of mRNA level and activity of GPx1 in bovine hepatocytes were evaluated, and the optimal doses of different forms of Se that supported the full expression of GPx1 were determined. Primary cultured bovine hepatocyte monolayers derived from neonatal male Holstein calves (aged 1-2 days) were incubated for 24 h with 0 (control), 0.5, 1, 1.5, 2, 3, 4 or 5 micromol/L of Se from dl-selenomethionine (Se-Met), sodium selenite (Na(2)SeO(3)) or Kappa-selenocarrageenan (Se-Car). Compared with controls, a significantly lower level of release of lactic dehydrogenase (LDH) was observed at 0.5-5 micromol/L of Se-Met, 0.5-1 micromol/L of Na(2)SeO(3) and 0.5 micromol/L of Se-Car, but significantly higher LDH release was observed at 2-5 micromol/L of Na(2)SeO(3) and 3-5 micromol/L of Se-Car, and the response occurred in a dose-dependent manner. The intracellular content of reduced glutathione in all hepatocytes treated with Se was significantly lower than that of controls. Significant increases in GPx1 mRNA were obtained in all hepatocytes treated with Se, with maximal effects at 3 micromol/L of Se-Met, 1.5 micromol/L of Na(2)SeO(3) and 2 micromol/L of Se-Car, respectively. Furthermore, 3 micromol/L of Se from Se-Met resulted in peak levels of GPx1 mRNA. After reaching a maximal level, higher Se supplementation led to a reduction of GPx1 mRNA. The activity of GPx1 showed similar patterns but of lower magnitude. We conclude that (a) the regulation of mRNA level and activity of GPx1 in primary cultured bovine hepatocytes by different forms of Se varies and (b) the optimal doses of Se to support the full expression of GPx1 in bovine hepatocytes when supplied as Se-Met, Na(2)SeO(3) and Se-Car are 3, 1.5 and 2 micromol/L, respectively.

Authors+Show Affiliations

Institute of Nutritional and Metabolic Disorders in Domestic Animals and Fowls, Nanjing Agricultural University, Nanjing 210095, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19269156

Citation

Wu, Xianshi, et al. "Regulation of Cellular Glutathione Peroxidase By Different Forms and Concentrations of Selenium in Primary Cultured Bovine Hepatocytes." The Journal of Nutritional Biochemistry, vol. 21, no. 2, 2010, pp. 153-61.
Wu X, Huang K, Wei C, et al. Regulation of cellular glutathione peroxidase by different forms and concentrations of selenium in primary cultured bovine hepatocytes. J Nutr Biochem. 2010;21(2):153-61.
Wu, X., Huang, K., Wei, C., Chen, F., & Pan, C. (2010). Regulation of cellular glutathione peroxidase by different forms and concentrations of selenium in primary cultured bovine hepatocytes. The Journal of Nutritional Biochemistry, 21(2), 153-61. https://doi.org/10.1016/j.jnutbio.2008.12.006
Wu X, et al. Regulation of Cellular Glutathione Peroxidase By Different Forms and Concentrations of Selenium in Primary Cultured Bovine Hepatocytes. J Nutr Biochem. 2010;21(2):153-61. PubMed PMID: 19269156.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation of cellular glutathione peroxidase by different forms and concentrations of selenium in primary cultured bovine hepatocytes. AU - Wu,Xianshi, AU - Huang,Kehe, AU - Wei,Chengwu, AU - Chen,Fu, AU - Pan,Cuiling, Y1 - 2009/03/09/ PY - 2008/05/22/received PY - 2008/12/07/revised PY - 2008/12/10/accepted PY - 2009/3/10/entrez PY - 2009/3/10/pubmed PY - 2010/4/30/medline SP - 153 EP - 61 JF - The Journal of nutritional biochemistry JO - J. Nutr. Biochem. VL - 21 IS - 2 N2 - The expression and activity of cellular glutathione peroxidase (GPx1) are regulated by selenium (Se). Generally speaking, organic forms of Se have less toxicity and greater bioavailability compared with inorganic forms. In this study, the effects of different forms and concentrations of Se on the regulation of mRNA level and activity of GPx1 in bovine hepatocytes were evaluated, and the optimal doses of different forms of Se that supported the full expression of GPx1 were determined. Primary cultured bovine hepatocyte monolayers derived from neonatal male Holstein calves (aged 1-2 days) were incubated for 24 h with 0 (control), 0.5, 1, 1.5, 2, 3, 4 or 5 micromol/L of Se from dl-selenomethionine (Se-Met), sodium selenite (Na(2)SeO(3)) or Kappa-selenocarrageenan (Se-Car). Compared with controls, a significantly lower level of release of lactic dehydrogenase (LDH) was observed at 0.5-5 micromol/L of Se-Met, 0.5-1 micromol/L of Na(2)SeO(3) and 0.5 micromol/L of Se-Car, but significantly higher LDH release was observed at 2-5 micromol/L of Na(2)SeO(3) and 3-5 micromol/L of Se-Car, and the response occurred in a dose-dependent manner. The intracellular content of reduced glutathione in all hepatocytes treated with Se was significantly lower than that of controls. Significant increases in GPx1 mRNA were obtained in all hepatocytes treated with Se, with maximal effects at 3 micromol/L of Se-Met, 1.5 micromol/L of Na(2)SeO(3) and 2 micromol/L of Se-Car, respectively. Furthermore, 3 micromol/L of Se from Se-Met resulted in peak levels of GPx1 mRNA. After reaching a maximal level, higher Se supplementation led to a reduction of GPx1 mRNA. The activity of GPx1 showed similar patterns but of lower magnitude. We conclude that (a) the regulation of mRNA level and activity of GPx1 in primary cultured bovine hepatocytes by different forms of Se varies and (b) the optimal doses of Se to support the full expression of GPx1 in bovine hepatocytes when supplied as Se-Met, Na(2)SeO(3) and Se-Car are 3, 1.5 and 2 micromol/L, respectively. SN - 1873-4847 UR - https://www.unboundmedicine.com/medline/citation/19269156/Regulation_of_cellular_glutathione_peroxidase_by_different_forms_and_concentrations_of_selenium_in_primary_cultured_bovine_hepatocytes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0955-2863(08)00266-0 DB - PRIME DP - Unbound Medicine ER -