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Aligned and random nanofibrous substrate for the in vitro culture of Schwann cells for neural tissue engineering.
Acta Biomater 2009; 5(7):2560-9AB

Abstract

The current challenge in peripheral nerve tissue engineering is to produce an implantable scaffold capable of bridging long nerve gaps that will produce results similar to autograft without requiring the harvest of autologous donor tissue. Aligned and random polycaprolactone/gelatin (PCL/gelatin) nanofibrous scaffolds were fabricated for the in vitro culture of Schwann cells that assist in directing the growth of regenerating axons in nerve tissue engineering. The average fiber diameter attained by electrospinning of polymer blend (PCL/gelatin) ranged from 232+/-194 to 160+/-86nm with high porosity (90%). Blending PCL with gelatin resulted in increased hydrophilicity of nanofibrous scaffolds and yielded better mechanical properties, approaching those of PCL nanofibers. The biocompatibility of fabricated nanofibers was assessed for culturing and proliferation of Schwann cells by MTS assay. The results of the MTS assay and scanning electron microscopy confirmed that aligned and random PCL/gelatin nanofibrous scaffolds are suitable substrates for Schwann cell growth as compared to PCL nanofibrous scaffolds for neural tissue engineering.

Authors+Show Affiliations

Nanoscience and Nanotechnology Initiative, Division of Bioengineering, National University of Singapore, Block E3, #05-12, 2 Engineering Drive 3, Singapore 117576.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19269270

Citation

Gupta, Deepika, et al. "Aligned and Random Nanofibrous Substrate for the in Vitro Culture of Schwann Cells for Neural Tissue Engineering." Acta Biomaterialia, vol. 5, no. 7, 2009, pp. 2560-9.
Gupta D, Venugopal J, Prabhakaran MP, et al. Aligned and random nanofibrous substrate for the in vitro culture of Schwann cells for neural tissue engineering. Acta Biomater. 2009;5(7):2560-9.
Gupta, D., Venugopal, J., Prabhakaran, M. P., Dev, V. R., Low, S., Choon, A. T., & Ramakrishna, S. (2009). Aligned and random nanofibrous substrate for the in vitro culture of Schwann cells for neural tissue engineering. Acta Biomaterialia, 5(7), pp. 2560-9. doi:10.1016/j.actbio.2009.01.039.
Gupta D, et al. Aligned and Random Nanofibrous Substrate for the in Vitro Culture of Schwann Cells for Neural Tissue Engineering. Acta Biomater. 2009;5(7):2560-9. PubMed PMID: 19269270.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Aligned and random nanofibrous substrate for the in vitro culture of Schwann cells for neural tissue engineering. AU - Gupta,Deepika, AU - Venugopal,J, AU - Prabhakaran,Molamma P, AU - Dev,V R Giri, AU - Low,Sharon, AU - Choon,Aw Tar, AU - Ramakrishna,S, Y1 - 2009/02/05/ PY - 2008/08/20/received PY - 2009/01/20/revised PY - 2009/01/26/accepted PY - 2009/3/10/entrez PY - 2009/3/10/pubmed PY - 2009/11/6/medline SP - 2560 EP - 9 JF - Acta biomaterialia JO - Acta Biomater VL - 5 IS - 7 N2 - The current challenge in peripheral nerve tissue engineering is to produce an implantable scaffold capable of bridging long nerve gaps that will produce results similar to autograft without requiring the harvest of autologous donor tissue. Aligned and random polycaprolactone/gelatin (PCL/gelatin) nanofibrous scaffolds were fabricated for the in vitro culture of Schwann cells that assist in directing the growth of regenerating axons in nerve tissue engineering. The average fiber diameter attained by electrospinning of polymer blend (PCL/gelatin) ranged from 232+/-194 to 160+/-86nm with high porosity (90%). Blending PCL with gelatin resulted in increased hydrophilicity of nanofibrous scaffolds and yielded better mechanical properties, approaching those of PCL nanofibers. The biocompatibility of fabricated nanofibers was assessed for culturing and proliferation of Schwann cells by MTS assay. The results of the MTS assay and scanning electron microscopy confirmed that aligned and random PCL/gelatin nanofibrous scaffolds are suitable substrates for Schwann cell growth as compared to PCL nanofibrous scaffolds for neural tissue engineering. SN - 1878-7568 UR - https://www.unboundmedicine.com/medline/citation/19269270/Aligned_and_random_nanofibrous_substrate_for_the_in_vitro_culture_of_Schwann_cells_for_neural_tissue_engineering_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1742-7061(09)00048-8 DB - PRIME DP - Unbound Medicine ER -