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A low-cost light-emitting diode induced fluorescence detector for capillary electrophoresis based on an orthogonal optical arrangement.
Talanta. 2009 May 15; 78(3):1155-8.T

Abstract

In this work, a simple and low-cost miniaturized light-emitting diode induced fluorescence (LED-IF) detector based on an orthogonal optical arrangement for capillary electrophoresis (CE) was developed, using a blue concave light-emitting diode (LED) as excitation source and a photodiode as photodetector. A lens obtained from a waste DVD-ROM was used to focus the LED light beam into an approximately 80 microm spot. Fluorescence was collected with an ocular obtained from a pen microscope at 45 degrees angle, and passed through a band-pass filter to a photodiode detector. The performance of the LED-IF detector was demonstrated in CE separations using sodium fluorescein and fluorescein isothiocyanate (FITC)-labeled amino acids as model samples. The limit of detection for sodium fluorescein was 0.92 microM with a signal-to-noise ratio (S/N) of 3. The total cost of the LED-IF detector was less than $ 50.

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Publisher Full Text

Authors+Show Affiliations

Yang FB
Institute of Microanalytical Systems, Zhejiang University, Hangzhou 310058, China.
Pan JZ
No affiliation info available
Zhang T
No affiliation info available
Fang Q
No affiliation info available

MeSH

Amino AcidsCosts and Cost AnalysisElectrophoresis, CapillaryEquipment DesignFluorescein-5-isothiocyanateFluoresceinsFluorescenceFluorescent DyesLight

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19269486

Citation

Yang, Feng-Bo, et al. "A Low-cost Light-emitting Diode Induced Fluorescence Detector for Capillary Electrophoresis Based On an Orthogonal Optical Arrangement." Talanta, vol. 78, no. 3, 2009, pp. 1155-8.
Yang FB, Pan JZ, Zhang T, et al. A low-cost light-emitting diode induced fluorescence detector for capillary electrophoresis based on an orthogonal optical arrangement. Talanta. 2009;78(3):1155-8.
Yang, F. B., Pan, J. Z., Zhang, T., & Fang, Q. (2009). A low-cost light-emitting diode induced fluorescence detector for capillary electrophoresis based on an orthogonal optical arrangement. Talanta, 78(3), 1155-8. https://doi.org/10.1016/j.talanta.2009.01.033
Yang FB, et al. A Low-cost Light-emitting Diode Induced Fluorescence Detector for Capillary Electrophoresis Based On an Orthogonal Optical Arrangement. Talanta. 2009 May 15;78(3):1155-8. PubMed PMID: 19269486.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A low-cost light-emitting diode induced fluorescence detector for capillary electrophoresis based on an orthogonal optical arrangement. AU - Yang,Feng-Bo, AU - Pan,Jian-Zhang, AU - Zhang,Ting, AU - Fang,Qun, Y1 - 2009/01/24/ PY - 2008/11/06/received PY - 2009/01/15/revised PY - 2009/01/18/accepted PY - 2009/3/10/entrez PY - 2009/3/10/pubmed PY - 2009/4/30/medline SP - 1155 EP - 8 JF - Talanta JO - Talanta VL - 78 IS - 3 N2 - In this work, a simple and low-cost miniaturized light-emitting diode induced fluorescence (LED-IF) detector based on an orthogonal optical arrangement for capillary electrophoresis (CE) was developed, using a blue concave light-emitting diode (LED) as excitation source and a photodiode as photodetector. A lens obtained from a waste DVD-ROM was used to focus the LED light beam into an approximately 80 microm spot. Fluorescence was collected with an ocular obtained from a pen microscope at 45 degrees angle, and passed through a band-pass filter to a photodiode detector. The performance of the LED-IF detector was demonstrated in CE separations using sodium fluorescein and fluorescein isothiocyanate (FITC)-labeled amino acids as model samples. The limit of detection for sodium fluorescein was 0.92 microM with a signal-to-noise ratio (S/N) of 3. The total cost of the LED-IF detector was less than $ 50. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/19269486/A_low_cost_light_emitting_diode_induced_fluorescence_detector_for_capillary_electrophoresis_based_on_an_orthogonal_optical_arrangement_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(09)00096-4 DB - PRIME DP - Unbound Medicine ER -