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Separation of tyrosine enantiomer derivatives by capillary electrophoresis with light-emitting diode-induced fluorescence detection.
Talanta. 2009 May 15; 78(3):1167-72.T

Abstract

Capillary electrophoresis (CE) coupled with fiber-optic light-emitting diode-induced fluorescence detection has been developed for the separation of tyrosine (Tyr) enantiomers. R(-)-4-(3-isothiocyanatopyrrolidin-1-yl)-7-(N,N-dimethylaminosulfonyl)-2,1,3-benzoxadiazole was used as a chiral fluorescence tagged reagent for derivatization of Tyr. The effect of pH, running buffer concentration and applied voltage on enantioselectivity has been investigated. The optimum CE conditions are 15 mmol/L borate running buffer (pH 10.5) and 14-kV applied voltage. Good reproducibility was obtained with coefficient of variation (n=7) of migration time and peak area less than 0.2 and 2.0%, respectively. The limits of detection of D- and L-Tyr derivatives were 2.9 and 2.2 micromol/L (S/N=3), respectively. The proposed method has been successfully applied to the determination of Tyr in a commercial amino acid oral solution.

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Authors+Show Affiliations

Bi W
College of Chemistry, Sichuan University, Chengdu 610065, PR China.
Lei S
No affiliation info available
Yang X
No affiliation info available
Xu Z
No affiliation info available
Yuan H
No affiliation info available
Xiao D
No affiliation info available
Choi MM
No affiliation info available

MeSH

Amino AcidsElectrophoresis, CapillaryEquipment DesignFluorescenceFluorescent DyesLightSolutionsStereoisomerismTyrosine

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19269488

Citation

Bi, Weiwen, et al. "Separation of Tyrosine Enantiomer Derivatives By Capillary Electrophoresis With Light-emitting Diode-induced Fluorescence Detection." Talanta, vol. 78, no. 3, 2009, pp. 1167-72.
Bi W, Lei S, Yang X, et al. Separation of tyrosine enantiomer derivatives by capillary electrophoresis with light-emitting diode-induced fluorescence detection. Talanta. 2009;78(3):1167-72.
Bi, W., Lei, S., Yang, X., Xu, Z., Yuan, H., Xiao, D., & Choi, M. M. (2009). Separation of tyrosine enantiomer derivatives by capillary electrophoresis with light-emitting diode-induced fluorescence detection. Talanta, 78(3), 1167-72. https://doi.org/10.1016/j.talanta.2009.01.042
Bi W, et al. Separation of Tyrosine Enantiomer Derivatives By Capillary Electrophoresis With Light-emitting Diode-induced Fluorescence Detection. Talanta. 2009 May 15;78(3):1167-72. PubMed PMID: 19269488.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Separation of tyrosine enantiomer derivatives by capillary electrophoresis with light-emitting diode-induced fluorescence detection. AU - Bi,Weiwen, AU - Lei,Shaorong, AU - Yang,Xiupei, AU - Xu,Zemin, AU - Yuan,Hongyan, AU - Xiao,Dan, AU - Choi,Martin M F, Y1 - 2009/01/30/ PY - 2008/09/25/received PY - 2009/01/20/revised PY - 2009/01/20/accepted PY - 2009/3/10/entrez PY - 2009/3/10/pubmed PY - 2009/4/30/medline SP - 1167 EP - 72 JF - Talanta JO - Talanta VL - 78 IS - 3 N2 - Capillary electrophoresis (CE) coupled with fiber-optic light-emitting diode-induced fluorescence detection has been developed for the separation of tyrosine (Tyr) enantiomers. R(-)-4-(3-isothiocyanatopyrrolidin-1-yl)-7-(N,N-dimethylaminosulfonyl)-2,1,3-benzoxadiazole was used as a chiral fluorescence tagged reagent for derivatization of Tyr. The effect of pH, running buffer concentration and applied voltage on enantioselectivity has been investigated. The optimum CE conditions are 15 mmol/L borate running buffer (pH 10.5) and 14-kV applied voltage. Good reproducibility was obtained with coefficient of variation (n=7) of migration time and peak area less than 0.2 and 2.0%, respectively. The limits of detection of D- and L-Tyr derivatives were 2.9 and 2.2 micromol/L (S/N=3), respectively. The proposed method has been successfully applied to the determination of Tyr in a commercial amino acid oral solution. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/19269488/Separation_of_tyrosine_enantiomer_derivatives_by_capillary_electrophoresis_with_light_emitting_diode_induced_fluorescence_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(09)00099-X DB - PRIME DP - Unbound Medicine ER -

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