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Prevalence of nasal carriage of methicillin-resistant Staphylococcus aureus and its antibiotic susceptibility pattern in healthcare workers at Namazi Hospital, Shiraz, Iran.
Int J Infect Dis. 2009 Sep; 13(5):e241-7.IJ

Abstract

OBJECTIVE

The aim of this study was to determine the prevalence of nasal carriage of methicillin-resistant Staphylococcus aureus (MRSA) among healthcare workers (HCWs) at Namazi Hospital, Shiraz, Iran.

METHODS

This cross-sectional study was conducted from July to November 2006. Nasal swabs were taken from 600 randomly selected HCWs. The isolates were identified as S. aureus based on morphology, Gram stain, catalase test, coagulase test, and mannitol salt agar fermentation. To analyze sensitivity patterns of MRSA strains more precisely, minimum inhibitory concentrations (MICs) of antibiotics were determined by the E-test method. All methicillin-resistant isolates were examined for the existence of the mecA gene by total DNA extraction and PCR.

RESULTS

The prevalence of nasal carriage of methicillin-sensitive S. aureus (MSSA) was 25.7% and of MRSA was 5.3%, with the highest nasal carriage of MRSA in surgical wards and the emergency department. There was no significant difference between the sexes (p=0.247), age (p=0.817), and years of healthcare service (p=0.15) with regard to the nasal carriage of MRSA and MSSA. In the univariate analysis, a statistically significant difference was only found for occupation (p=0.032) between the carriage of MSSA and MRSA. In the multivariate analysis, the occupation 'nurse' was independently associated with MRSA carriage (p=0.012, odds ratio 3.6, 95% confidence interval 1.3-9.7). The highest resistance rate for both gentamicin and clindamycin (69%) was noted among the MRSA strains. None of the MRSA strains were resistant to mupirocin, linezolid, fusidic acid, or vancomycin. The existence of the mecA gene in all 32 methicillin-resistant isolates was observed by PCR.

CONCLUSIONS

This study revealed the prevalence of nasal carriage of S. aureus strains among HCWs to be lower than that found in other studies from Iran. The antibiotic susceptibility patterns also differed, perhaps as a result of the excessive use of antibiotics at our hospital. Only the occupation of nurse was an independent risk factor for MRSA carriage.

Authors+Show Affiliations

Department of Community Medicine, Shiraz University of Medical Sciences, PO Box 71345-1737, Shiraz, Iran. askariam@sums.ac.irNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19269873

Citation

Askarian, Mehrdad, et al. "Prevalence of Nasal Carriage of Methicillin-resistant Staphylococcus Aureus and Its Antibiotic Susceptibility Pattern in Healthcare Workers at Namazi Hospital, Shiraz, Iran." International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases, vol. 13, no. 5, 2009, pp. e241-7.
Askarian M, Zeinalzadeh A, Japoni A, et al. Prevalence of nasal carriage of methicillin-resistant Staphylococcus aureus and its antibiotic susceptibility pattern in healthcare workers at Namazi Hospital, Shiraz, Iran. Int J Infect Dis. 2009;13(5):e241-7.
Askarian, M., Zeinalzadeh, A., Japoni, A., Alborzi, A., & Memish, Z. A. (2009). Prevalence of nasal carriage of methicillin-resistant Staphylococcus aureus and its antibiotic susceptibility pattern in healthcare workers at Namazi Hospital, Shiraz, Iran. International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases, 13(5), e241-7. https://doi.org/10.1016/j.ijid.2008.11.026
Askarian M, et al. Prevalence of Nasal Carriage of Methicillin-resistant Staphylococcus Aureus and Its Antibiotic Susceptibility Pattern in Healthcare Workers at Namazi Hospital, Shiraz, Iran. Int J Infect Dis. 2009;13(5):e241-7. PubMed PMID: 19269873.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prevalence of nasal carriage of methicillin-resistant Staphylococcus aureus and its antibiotic susceptibility pattern in healthcare workers at Namazi Hospital, Shiraz, Iran. AU - Askarian,Mehrdad, AU - Zeinalzadeh,Alihosein, AU - Japoni,Aziz, AU - Alborzi,Abdolvahab, AU - Memish,Ziad A, Y1 - 2009/03/09/ PY - 2008/02/15/received PY - 2008/10/28/revised PY - 2008/11/17/accepted PY - 2009/3/10/entrez PY - 2009/3/10/pubmed PY - 2009/12/16/medline SP - e241 EP - 7 JF - International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases JO - Int. J. Infect. Dis. VL - 13 IS - 5 N2 - OBJECTIVE: The aim of this study was to determine the prevalence of nasal carriage of methicillin-resistant Staphylococcus aureus (MRSA) among healthcare workers (HCWs) at Namazi Hospital, Shiraz, Iran. METHODS: This cross-sectional study was conducted from July to November 2006. Nasal swabs were taken from 600 randomly selected HCWs. The isolates were identified as S. aureus based on morphology, Gram stain, catalase test, coagulase test, and mannitol salt agar fermentation. To analyze sensitivity patterns of MRSA strains more precisely, minimum inhibitory concentrations (MICs) of antibiotics were determined by the E-test method. All methicillin-resistant isolates were examined for the existence of the mecA gene by total DNA extraction and PCR. RESULTS: The prevalence of nasal carriage of methicillin-sensitive S. aureus (MSSA) was 25.7% and of MRSA was 5.3%, with the highest nasal carriage of MRSA in surgical wards and the emergency department. There was no significant difference between the sexes (p=0.247), age (p=0.817), and years of healthcare service (p=0.15) with regard to the nasal carriage of MRSA and MSSA. In the univariate analysis, a statistically significant difference was only found for occupation (p=0.032) between the carriage of MSSA and MRSA. In the multivariate analysis, the occupation 'nurse' was independently associated with MRSA carriage (p=0.012, odds ratio 3.6, 95% confidence interval 1.3-9.7). The highest resistance rate for both gentamicin and clindamycin (69%) was noted among the MRSA strains. None of the MRSA strains were resistant to mupirocin, linezolid, fusidic acid, or vancomycin. The existence of the mecA gene in all 32 methicillin-resistant isolates was observed by PCR. CONCLUSIONS: This study revealed the prevalence of nasal carriage of S. aureus strains among HCWs to be lower than that found in other studies from Iran. The antibiotic susceptibility patterns also differed, perhaps as a result of the excessive use of antibiotics at our hospital. Only the occupation of nurse was an independent risk factor for MRSA carriage. SN - 1878-3511 UR - https://www.unboundmedicine.com/medline/citation/19269873/Prevalence_of_nasal_carriage_of_methicillin_resistant_Staphylococcus_aureus_and_its_antibiotic_susceptibility_pattern_in_healthcare_workers_at_Namazi_Hospital_Shiraz_Iran_ L2 - http://linkinghub.elsevier.com/retrieve/pii/S1201-9712(09)00043-5 DB - PRIME DP - Unbound Medicine ER -