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The Magnaporthe oryzae avirulence gene AvrPiz-t encodes a predicted secreted protein that triggers the immunity in rice mediated by the blast resistance gene Piz-t.
Mol Plant Microbe Interact. 2009 Apr; 22(4):411-20.MP

Abstract

The Magnaporthe oryzae avirulence gene AvrPiz-t activates immunity in a gene-for-gene fashion to rice mediated by the blast resistance gene Piz-t. To dissect the molecular mechanism underlying their recognition, we initiated the cloning of AvrPiz-t using a map-based cloning strategy. The AvrPiz-t gene was delimited to an approximately 21-kb genomic fragment, in which six genes were predicted. Complementation tests of each of these six candidate genes led to the final identification of AvrPiz-t, which encodes a 108-amino-acid predicted secreted protein with unknown function and no homologues in M. oryzae or in other sequenced fungi. We found that AvrPiz-t is present in the virulent isolate GUY11 but contains a Pot3 insertion at a position 462 bp upstream from the start codon. Complementation tests of AvrPiz-t genes driven by promoters of varying length revealed that a promoter larger than 462 bp is essential to maintain the AvrPiz-t function. These results suggest that a Pot3 insertion in GUY11 might interfere with the proper function of AvrPiz-t. Additionally, we found that AvrPiz-t can suppress the programmed cell death triggered by mouse BAX protein in Nicotiana benthamiana, identifying a mechanism by which AvrPiz-t may contribute virulence of M. oryzae.

Authors+Show Affiliations

National Center for Gene Research & Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19271956

Citation

Li, Wei, et al. "The Magnaporthe Oryzae Avirulence Gene AvrPiz-t Encodes a Predicted Secreted Protein That Triggers the Immunity in Rice Mediated By the Blast Resistance Gene Piz-t." Molecular Plant-microbe Interactions : MPMI, vol. 22, no. 4, 2009, pp. 411-20.
Li W, Wang B, Wu J, et al. The Magnaporthe oryzae avirulence gene AvrPiz-t encodes a predicted secreted protein that triggers the immunity in rice mediated by the blast resistance gene Piz-t. Mol Plant Microbe Interact. 2009;22(4):411-20.
Li, W., Wang, B., Wu, J., Lu, G., Hu, Y., Zhang, X., Zhang, Z., Zhao, Q., Feng, Q., Zhang, H., Wang, Z., Wang, G., Han, B., Wang, Z., & Zhou, B. (2009). The Magnaporthe oryzae avirulence gene AvrPiz-t encodes a predicted secreted protein that triggers the immunity in rice mediated by the blast resistance gene Piz-t. Molecular Plant-microbe Interactions : MPMI, 22(4), 411-20. https://doi.org/10.1094/MPMI-22-4-0411
Li W, et al. The Magnaporthe Oryzae Avirulence Gene AvrPiz-t Encodes a Predicted Secreted Protein That Triggers the Immunity in Rice Mediated By the Blast Resistance Gene Piz-t. Mol Plant Microbe Interact. 2009;22(4):411-20. PubMed PMID: 19271956.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Magnaporthe oryzae avirulence gene AvrPiz-t encodes a predicted secreted protein that triggers the immunity in rice mediated by the blast resistance gene Piz-t. AU - Li,Wei, AU - Wang,Baohua, AU - Wu,Jun, AU - Lu,Guodong, AU - Hu,Yajun, AU - Zhang,Xing, AU - Zhang,Zhengguang, AU - Zhao,Qiang, AU - Feng,Qi, AU - Zhang,Hongyan, AU - Wang,Zhengyi, AU - Wang,Guoliang, AU - Han,Bin, AU - Wang,Zonghua, AU - Zhou,Bo, PY - 2009/3/11/entrez PY - 2009/3/11/pubmed PY - 2009/5/7/medline SP - 411 EP - 20 JF - Molecular plant-microbe interactions : MPMI JO - Mol Plant Microbe Interact VL - 22 IS - 4 N2 - The Magnaporthe oryzae avirulence gene AvrPiz-t activates immunity in a gene-for-gene fashion to rice mediated by the blast resistance gene Piz-t. To dissect the molecular mechanism underlying their recognition, we initiated the cloning of AvrPiz-t using a map-based cloning strategy. The AvrPiz-t gene was delimited to an approximately 21-kb genomic fragment, in which six genes were predicted. Complementation tests of each of these six candidate genes led to the final identification of AvrPiz-t, which encodes a 108-amino-acid predicted secreted protein with unknown function and no homologues in M. oryzae or in other sequenced fungi. We found that AvrPiz-t is present in the virulent isolate GUY11 but contains a Pot3 insertion at a position 462 bp upstream from the start codon. Complementation tests of AvrPiz-t genes driven by promoters of varying length revealed that a promoter larger than 462 bp is essential to maintain the AvrPiz-t function. These results suggest that a Pot3 insertion in GUY11 might interfere with the proper function of AvrPiz-t. Additionally, we found that AvrPiz-t can suppress the programmed cell death triggered by mouse BAX protein in Nicotiana benthamiana, identifying a mechanism by which AvrPiz-t may contribute virulence of M. oryzae. SN - 0894-0282 UR - https://www.unboundmedicine.com/medline/citation/19271956/The_Magnaporthe_oryzae_avirulence_gene_AvrPiz_t_encodes_a_predicted_secreted_protein_that_triggers_the_immunity_in_rice_mediated_by_the_blast_resistance_gene_Piz_t_ L2 - https://apsjournals.apsnet.org/doi/10.1094/MPMI-22-4-0411?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -