TY - JOUR T1 - Isolation of isomangiferin from honeybush (Cyclopia subternata) using high-speed counter-current chromatography and high-performance liquid chromatography. AU - de Beer,Dalene, AU - Jerz,Gerold, AU - Joubert,Elizabeth, AU - Wray,Victor, AU - Winterhalter,Peter, Y1 - 2009/02/24/ PY - 2008/09/30/received PY - 2009/01/27/revised PY - 2009/02/19/accepted PY - 2009/3/11/entrez PY - 2009/3/11/pubmed PY - 2009/7/16/medline SP - 4282 EP - 9 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1216 IS - 19 N2 - Isomangiferin was isolated from Cyclopia subternata using a multi-step process including extraction, liquid-liquid partitioning, high-speed counter-current chromatography (HSCCC) and semi-preparative reversed-phase high-performance liquid chromatography (HPLC). Enrichment of phenolic compounds in a methanol extract of C. subternata leaves was conducted using liquid-liquid partitioning with ethyl acetate-methanol-water (1:1:2, v/v). The enriched fraction was further fractionated using HSCCC with a ternary solvent system consisting of tert-butyl methyl ether-n-butanol-acetonitrile-water (3:1:1:5, v/v). Isomangiferin was isolated by semi-preparative reversed-phase HPLC from a fraction containing mostly mangiferin and isomangiferin. The chemical structure of isomangiferin was confirmed by LC-high-resolution electrospray ionization MS, as well as one- and two-dimensional NMR spectroscopy. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/19272608/Isolation_of_isomangiferin_from_honeybush__Cyclopia_subternata__using_high_speed_counter_current_chromatography_and_high_performance_liquid_chromatography_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(09)00302-1 DB - PRIME DP - Unbound Medicine ER -