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Molecular epidemiology and antimicrobial susceptibility of extended- and broad-spectrum beta-lactamase-producing Klebsiella pneumoniae isolated in Portugal.
Int J Antimicrob Agents. 2009 Jul; 34(1):29-37.IJ

Abstract

All 187 Klebsiella pneumoniae isolated over six consecutive months of 1999 in 17 Portuguese health institutions were studied: 89% were resistant to ampicillin, 31% to trimethoprim/sulfamethoxazole, 17% to aminoglycosides and 3% to fluoroquinolones; 16% were multidrug-resistant and 14% expressed an extended-spectrum beta-lactamase (ESBL) phenotype confirmed by genotyping. Molecular methods identified: 11 isolates possessing bla(ESBL-SHV) genes (bla(SHV-2A), bla(SHV-5), bla(SHV-12) and bla(SHV-55)), 9 isolates with bla(ESBL-TEM) (bla(TEM-3), bla(TEM-10) and bla(TEM-24)) and 7 isolates with bla(GES-1), encoding ESBL enzymes; and 160 isolates with bla(SHV-1) and bla(SHV-type) encoding non-ESBL enzymes. Overall, 15 new beta-lactamases were detected: SHV-60 to SHV-62, SHV-71 and SHV-73 to SHV-83. The genetic relatedness of 108 isolates was studied by pulsed-field gel electrophoresis (PFGE) analysis. The isolates were diverse and 18 clusters were defined, the largest including 12 isolates of different specimens, 6 of which expressed GES-1 enzymes. Twenty additional strains isolated during a second period (March-November 2006) in three of the participating hospitals contained ESBL-encoding genes, whereas none of the isolates in the same hospitals in 1999 carried such genes: bla(SHV-5), bla(SHV-12), bla(TEM-10), bla(TEM-52), bla(CTX-M-15), bla(CTX-M-32) and bla(CTX-M-61) (first described in the country). In this period, three new enzymes were detected: SHV-106 to SHV-108. We provide evidence that the genotypes of K. pneumoniae isolates is changing towards the emergence of ESBL enzymes.

Authors+Show Affiliations

Antibiotic Resistance Unit, Department of Infectious Diseases, National Institute of Health Dr Ricardo Jorge, Av. Padre Cruz, 1649-016 Lisbon, Portugal.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19272757

Citation

Mendonça, Nuno, et al. "Molecular Epidemiology and Antimicrobial Susceptibility of Extended- and Broad-spectrum Beta-lactamase-producing Klebsiella Pneumoniae Isolated in Portugal." International Journal of Antimicrobial Agents, vol. 34, no. 1, 2009, pp. 29-37.
Mendonça N, Ferreira E, Louro D, et al. Molecular epidemiology and antimicrobial susceptibility of extended- and broad-spectrum beta-lactamase-producing Klebsiella pneumoniae isolated in Portugal. Int J Antimicrob Agents. 2009;34(1):29-37.
Mendonça, N., Ferreira, E., Louro, D., & Caniça, M. (2009). Molecular epidemiology and antimicrobial susceptibility of extended- and broad-spectrum beta-lactamase-producing Klebsiella pneumoniae isolated in Portugal. International Journal of Antimicrobial Agents, 34(1), 29-37. https://doi.org/10.1016/j.ijantimicag.2008.11.014
Mendonça N, et al. Molecular Epidemiology and Antimicrobial Susceptibility of Extended- and Broad-spectrum Beta-lactamase-producing Klebsiella Pneumoniae Isolated in Portugal. Int J Antimicrob Agents. 2009;34(1):29-37. PubMed PMID: 19272757.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular epidemiology and antimicrobial susceptibility of extended- and broad-spectrum beta-lactamase-producing Klebsiella pneumoniae isolated in Portugal. AU - Mendonça,Nuno, AU - Ferreira,Eugénia, AU - Louro,Deolinda, AU - ,, AU - Caniça,Manuela, Y1 - 2009/03/09/ PY - 2008/07/22/received PY - 2008/11/25/revised PY - 2008/11/26/accepted PY - 2009/3/11/entrez PY - 2009/3/11/pubmed PY - 2009/7/11/medline SP - 29 EP - 37 JF - International journal of antimicrobial agents JO - Int J Antimicrob Agents VL - 34 IS - 1 N2 - All 187 Klebsiella pneumoniae isolated over six consecutive months of 1999 in 17 Portuguese health institutions were studied: 89% were resistant to ampicillin, 31% to trimethoprim/sulfamethoxazole, 17% to aminoglycosides and 3% to fluoroquinolones; 16% were multidrug-resistant and 14% expressed an extended-spectrum beta-lactamase (ESBL) phenotype confirmed by genotyping. Molecular methods identified: 11 isolates possessing bla(ESBL-SHV) genes (bla(SHV-2A), bla(SHV-5), bla(SHV-12) and bla(SHV-55)), 9 isolates with bla(ESBL-TEM) (bla(TEM-3), bla(TEM-10) and bla(TEM-24)) and 7 isolates with bla(GES-1), encoding ESBL enzymes; and 160 isolates with bla(SHV-1) and bla(SHV-type) encoding non-ESBL enzymes. Overall, 15 new beta-lactamases were detected: SHV-60 to SHV-62, SHV-71 and SHV-73 to SHV-83. The genetic relatedness of 108 isolates was studied by pulsed-field gel electrophoresis (PFGE) analysis. The isolates were diverse and 18 clusters were defined, the largest including 12 isolates of different specimens, 6 of which expressed GES-1 enzymes. Twenty additional strains isolated during a second period (March-November 2006) in three of the participating hospitals contained ESBL-encoding genes, whereas none of the isolates in the same hospitals in 1999 carried such genes: bla(SHV-5), bla(SHV-12), bla(TEM-10), bla(TEM-52), bla(CTX-M-15), bla(CTX-M-32) and bla(CTX-M-61) (first described in the country). In this period, three new enzymes were detected: SHV-106 to SHV-108. We provide evidence that the genotypes of K. pneumoniae isolates is changing towards the emergence of ESBL enzymes. SN - 1872-7913 UR - https://www.unboundmedicine.com/medline/citation/19272757/Molecular_epidemiology_and_antimicrobial_susceptibility_of_extended__and_broad_spectrum_beta_lactamase_producing_Klebsiella_pneumoniae_isolated_in_Portugal_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0924-8579(09)00018-1 DB - PRIME DP - Unbound Medicine ER -