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Vacuum-assisted closure therapy increases local interleukin-8 and vascular endothelial growth factor levels in traumatic wounds.
J Trauma 2009; 66(3):749-57JT

Abstract

BACKGROUND

Clinical observations are suggesting accelerated granulation tissue formation in traumatic wounds treated with vacuum-assisted closure (VAC). Aim of this study was to determine the impact of VAC therapy versus alternative Epigard application on local inflammation and neovascularization in traumatic soft tissue wounds.

METHODS

Thirty-two patients with traumatic wounds requiring temporary coverage (VAC n = 16; Epigard n = 16) were included. At each change of dressing, samples of wound fluid and serum were collected (n = 80). The cytokines interleukin (IL)-6, IL-8, vascular endothelial growth factor (VEGF), and fibroblast growth factor-2 were measured by ELISA. Wound biopsies were examined histologically for inflammatory cells and degree of neovascularization present.

RESULTS

All cytokines were found to be elevated in wound fluids during both VAC and Epigard treatment, whereas serum concentrations were negligible or not detectable. In wound fluids, significantly higher IL-8 (p < 0.001) and VEGF (p < 0.05) levels were detected during VAC therapy. Furthermore, histologic examination revealed increased neovascularization (p < 0.05) illustrated by CD31 and von Willebrand factor immunohistochemistry in wound biopsies of VAC treatment. In addition, there was an accumulation of neutrophils as well as an augmented expression of VEGF (p < 0.005) in VAC wound biopsies.

CONCLUSION

This study suggests that VAC therapy of traumatic wounds leads to increased local IL-8 and VEGF concentrations, which may trigger accumulation of neutrophils and angiogenesis and thus, accelerate neovascularization.

Authors+Show Affiliations

Division of Trauma Surgery, Department of Surgery, University Hospital Zürich, Zürich, Switzerland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19276749

Citation

Labler, Ludwig, et al. "Vacuum-assisted Closure Therapy Increases Local Interleukin-8 and Vascular Endothelial Growth Factor Levels in Traumatic Wounds." The Journal of Trauma, vol. 66, no. 3, 2009, pp. 749-57.
Labler L, Rancan M, Mica L, et al. Vacuum-assisted closure therapy increases local interleukin-8 and vascular endothelial growth factor levels in traumatic wounds. J Trauma. 2009;66(3):749-57.
Labler, L., Rancan, M., Mica, L., Härter, L., Mihic-Probst, D., & Keel, M. (2009). Vacuum-assisted closure therapy increases local interleukin-8 and vascular endothelial growth factor levels in traumatic wounds. The Journal of Trauma, 66(3), pp. 749-57. doi:10.1097/TA.0b013e318171971a.
Labler L, et al. Vacuum-assisted Closure Therapy Increases Local Interleukin-8 and Vascular Endothelial Growth Factor Levels in Traumatic Wounds. J Trauma. 2009;66(3):749-57. PubMed PMID: 19276749.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Vacuum-assisted closure therapy increases local interleukin-8 and vascular endothelial growth factor levels in traumatic wounds. AU - Labler,Ludwig, AU - Rancan,Mario, AU - Mica,Ladislav, AU - Härter,Luc, AU - Mihic-Probst,Daniela, AU - Keel,Marius, PY - 2009/3/12/entrez PY - 2009/3/12/pubmed PY - 2009/3/31/medline SP - 749 EP - 57 JF - The Journal of trauma JO - J Trauma VL - 66 IS - 3 N2 - BACKGROUND: Clinical observations are suggesting accelerated granulation tissue formation in traumatic wounds treated with vacuum-assisted closure (VAC). Aim of this study was to determine the impact of VAC therapy versus alternative Epigard application on local inflammation and neovascularization in traumatic soft tissue wounds. METHODS: Thirty-two patients with traumatic wounds requiring temporary coverage (VAC n = 16; Epigard n = 16) were included. At each change of dressing, samples of wound fluid and serum were collected (n = 80). The cytokines interleukin (IL)-6, IL-8, vascular endothelial growth factor (VEGF), and fibroblast growth factor-2 were measured by ELISA. Wound biopsies were examined histologically for inflammatory cells and degree of neovascularization present. RESULTS: All cytokines were found to be elevated in wound fluids during both VAC and Epigard treatment, whereas serum concentrations were negligible or not detectable. In wound fluids, significantly higher IL-8 (p < 0.001) and VEGF (p < 0.05) levels were detected during VAC therapy. Furthermore, histologic examination revealed increased neovascularization (p < 0.05) illustrated by CD31 and von Willebrand factor immunohistochemistry in wound biopsies of VAC treatment. In addition, there was an accumulation of neutrophils as well as an augmented expression of VEGF (p < 0.005) in VAC wound biopsies. CONCLUSION: This study suggests that VAC therapy of traumatic wounds leads to increased local IL-8 and VEGF concentrations, which may trigger accumulation of neutrophils and angiogenesis and thus, accelerate neovascularization. SN - 1529-8809 UR - https://www.unboundmedicine.com/medline/citation/19276749/Vacuum_assisted_closure_therapy_increases_local_interleukin_8_and_vascular_endothelial_growth_factor_levels_in_traumatic_wounds_ L2 - http://Insights.ovid.com/pubmed?pmid=19276749 DB - PRIME DP - Unbound Medicine ER -