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A sensitive and specific liquid chromatography/tandem mass spectrometry method for determination of echinacoside and its pharmacokinetic application in rats.
Biomed Chromatogr. 2009 Jun; 23(6):630-7.BC

Abstract

A rapid and sensitive method based on liquid chromatography/tandem mass spectrometry (LC/MS/MS) for the determination of echinacoside in rat plasma was established and fully validated. A single step of liquid-liquid extraction with n-butanol was utilized. Chromatographic separation of the analyte and the internal standard (IS), chlorogenic acid, from the sample matrix was performed using a Capcell-MG C(18) analytical column (100 2.0 mm x 5 microm), with a gradient of acetonitrile and water containing 0.1% acetic acid as the mobile phase. Detection was performed on a triple quadrupole tandem mass spectrometer equipped with electrospray ionization source operated in negative ion selected reaction monitoring mode. The method was linear in the concentration range 10-2500 ng/mL. The deviations of both intra- and inter-day precisions (RSD) were 7.1% and the assay accuracies were within 99.2-106.5%. Echinacoside proved to be stable during sample storage, preparation and analysis when an antioxidant solution was used. The method was successfully applied to a pharmacokinetic study in rats after an intragastric administration of echinacoside (100 mg/kg). With the lower limit of quantification at 10 ng/mL, this method proved to have sufficient selectivity, sensitivity and reproducibility for the pharmacokinetic study of echinacoside.

Authors+Show Affiliations

Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing 210038, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

19277965

Citation

Yang, Hao, et al. "A Sensitive and Specific Liquid Chromatography/tandem Mass Spectrometry Method for Determination of Echinacoside and Its Pharmacokinetic Application in Rats." Biomedical Chromatography : BMC, vol. 23, no. 6, 2009, pp. 630-7.
Yang H, Wang G, Hao H, et al. A sensitive and specific liquid chromatography/tandem mass spectrometry method for determination of echinacoside and its pharmacokinetic application in rats. Biomed Chromatogr. 2009;23(6):630-7.
Yang, H., Wang, G., Hao, H., Tu, P., Jiang, Y., Wang, Q., Zhang, Y., Zheng, C., Wang, Y., & Dai, L. (2009). A sensitive and specific liquid chromatography/tandem mass spectrometry method for determination of echinacoside and its pharmacokinetic application in rats. Biomedical Chromatography : BMC, 23(6), 630-7. https://doi.org/10.1002/bmc.1164
Yang H, et al. A Sensitive and Specific Liquid Chromatography/tandem Mass Spectrometry Method for Determination of Echinacoside and Its Pharmacokinetic Application in Rats. Biomed Chromatogr. 2009;23(6):630-7. PubMed PMID: 19277965.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A sensitive and specific liquid chromatography/tandem mass spectrometry method for determination of echinacoside and its pharmacokinetic application in rats. AU - Yang,Hao, AU - Wang,Guangji, AU - Hao,Haiping, AU - Tu,Pengfei, AU - Jiang,Yong, AU - Wang,Qiong, AU - Zhang,Yan, AU - Zheng,Chaonan, AU - Wang,Yuxin, AU - Dai,Liang, PY - 2009/3/12/entrez PY - 2009/3/12/pubmed PY - 2009/7/30/medline SP - 630 EP - 7 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 23 IS - 6 N2 - A rapid and sensitive method based on liquid chromatography/tandem mass spectrometry (LC/MS/MS) for the determination of echinacoside in rat plasma was established and fully validated. A single step of liquid-liquid extraction with n-butanol was utilized. Chromatographic separation of the analyte and the internal standard (IS), chlorogenic acid, from the sample matrix was performed using a Capcell-MG C(18) analytical column (100 2.0 mm x 5 microm), with a gradient of acetonitrile and water containing 0.1% acetic acid as the mobile phase. Detection was performed on a triple quadrupole tandem mass spectrometer equipped with electrospray ionization source operated in negative ion selected reaction monitoring mode. The method was linear in the concentration range 10-2500 ng/mL. The deviations of both intra- and inter-day precisions (RSD) were 7.1% and the assay accuracies were within 99.2-106.5%. Echinacoside proved to be stable during sample storage, preparation and analysis when an antioxidant solution was used. The method was successfully applied to a pharmacokinetic study in rats after an intragastric administration of echinacoside (100 mg/kg). With the lower limit of quantification at 10 ng/mL, this method proved to have sufficient selectivity, sensitivity and reproducibility for the pharmacokinetic study of echinacoside. SN - 1099-0801 UR - https://www.unboundmedicine.com/medline/citation/19277965/A_sensitive_and_specific_liquid_chromatography/tandem_mass_spectrometry_method_for_determination_of_echinacoside_and_its_pharmacokinetic_application_in_rats_ L2 - https://doi.org/10.1002/bmc.1164 DB - PRIME DP - Unbound Medicine ER -