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Free radical scavenging and antielastase activities of flavonoids from the fruits of Thuja orientalis.
Arch Pharm Res. 2009 Feb; 32(2):275-82.AP

Abstract

Bioassay-guided fractionation of the MeOH extract of Thuja orientalis fruits using a DPPH (2,2-diphenyl-1-picrylhydrazyl) assay led to the isolation of 9 flavonoids: cupressuflavone (1), amentoflavone (2), robustaflavone (3), afzelin (4), (+)-catechin (5), quercitrin (6), hypolaetin 7-O-beta-xylopyranoside (7), isoquercitrin (8) and myricitrin (9). Their chemical structures were determined by spectroscopic analyses. The free radical scavenging and human neutrophil elastase (HNE) inhibitory activities were evaluated for the isolated compounds. By DPPH scavenging assay, compounds 5, 6, 7, 8 and 9 showed anti-oxidant activities with IC(50) values of 28.66, 31.19, 18.30, 26.63 and 15.10 microM, respectively. By ABTS [2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt] scavenging assay, these compounds also exhibited potent anti-oxidant activities with IC(50) values of 6.77, 13.96, 6.97, 22.79 and 9.96 microM, respectively. Of note, compounds 1, 2 and 3 showed significant HNE inhibitory activities with IC(50) values of 8.09, 1.27 and 1.33 microM, respectively.

Authors+Show Affiliations

Functional Metabolite Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19280159

Citation

Xu, Guang-Hua, et al. "Free Radical Scavenging and Antielastase Activities of Flavonoids From the Fruits of Thuja Orientalis." Archives of Pharmacal Research, vol. 32, no. 2, 2009, pp. 275-82.
Xu GH, Ryoo IJ, Kim YH, et al. Free radical scavenging and antielastase activities of flavonoids from the fruits of Thuja orientalis. Arch Pharm Res. 2009;32(2):275-82.
Xu, G. H., Ryoo, I. J., Kim, Y. H., Choo, S. J., & Yoo, I. D. (2009). Free radical scavenging and antielastase activities of flavonoids from the fruits of Thuja orientalis. Archives of Pharmacal Research, 32(2), 275-82. https://doi.org/10.1007/s12272-009-1233-y
Xu GH, et al. Free Radical Scavenging and Antielastase Activities of Flavonoids From the Fruits of Thuja Orientalis. Arch Pharm Res. 2009;32(2):275-82. PubMed PMID: 19280159.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Free radical scavenging and antielastase activities of flavonoids from the fruits of Thuja orientalis. AU - Xu,Guang-Hua, AU - Ryoo,In-Ja, AU - Kim,Young-Hee, AU - Choo,Soo-Jin, AU - Yoo,Ick-Dong, Y1 - 2009/03/13/ PY - 2008/11/15/received PY - 2009/02/04/accepted PY - 2009/01/09/revised PY - 2009/3/13/entrez PY - 2009/3/13/pubmed PY - 2009/5/1/medline SP - 275 EP - 82 JF - Archives of pharmacal research JO - Arch Pharm Res VL - 32 IS - 2 N2 - Bioassay-guided fractionation of the MeOH extract of Thuja orientalis fruits using a DPPH (2,2-diphenyl-1-picrylhydrazyl) assay led to the isolation of 9 flavonoids: cupressuflavone (1), amentoflavone (2), robustaflavone (3), afzelin (4), (+)-catechin (5), quercitrin (6), hypolaetin 7-O-beta-xylopyranoside (7), isoquercitrin (8) and myricitrin (9). Their chemical structures were determined by spectroscopic analyses. The free radical scavenging and human neutrophil elastase (HNE) inhibitory activities were evaluated for the isolated compounds. By DPPH scavenging assay, compounds 5, 6, 7, 8 and 9 showed anti-oxidant activities with IC(50) values of 28.66, 31.19, 18.30, 26.63 and 15.10 microM, respectively. By ABTS [2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt] scavenging assay, these compounds also exhibited potent anti-oxidant activities with IC(50) values of 6.77, 13.96, 6.97, 22.79 and 9.96 microM, respectively. Of note, compounds 1, 2 and 3 showed significant HNE inhibitory activities with IC(50) values of 8.09, 1.27 and 1.33 microM, respectively. SN - 0253-6269 UR - https://www.unboundmedicine.com/medline/citation/19280159/Free_radical_scavenging_and_antielastase_activities_of_flavonoids_from_the_fruits_of_Thuja_orientalis_ L2 - https://dx.doi.org/10.1007/s12272-009-1233-y DB - PRIME DP - Unbound Medicine ER -