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Practical guidelines for characterization of O-diglycosyl flavonoid isomers by triple quadrupole MS and their applications for identification of some fruit juices flavonoids.
J Mass Spectrom. 2009 Jul; 44(7):1017-25.JM

Abstract

Fifteen flavonoid O-diglycosides with different interglycosidic linkage isomery and glycosylation position have been studied in order to analyze their fragmentation patterns. Initial separation was carried out using high performance liquid chromatography with diode array detection (HPLC/DAD) coupled to an electrospray ionization (ESI) interface and a triple quadrupole mass spectrometer. Some useful differences in their MS spectra have been found and discussed. As it has already been reported, [Y*]+/[Y0]+ ratio for flavanones and [Y1]+/[Y0]+ ratio for other flavonoids is specific for each isomeric interglycosidic linkage. In this work it has also been observed that the abundance of these ions is dependent on the position of glycosylation. On the basis of these differences, systematic guidelines for our experimental conditions have been proposed for the differentiation of not only isomeric interglycosidic linkage but also glycosylation position using collision-induced dissociation MS/MS (CID-MS/MS) spectra in positive mode. These results have been successfully applied for the characterization of three diglycosyl flavonoids found in Citrus fruit juices and these conclusions have also been extrapolated for characterizing two triglycosides in the same fruits.

Authors+Show Affiliations

Departamento de Química Analítica, Facultad de Ciencia y Tecnología, Universidad del País Vasco/Euskal Herriko Unibertsitatea, P.O. Box 644, 48080 Bilbao, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19283783

Citation

Abad-García, B, et al. "Practical Guidelines for Characterization of O-diglycosyl Flavonoid Isomers By Triple Quadrupole MS and Their Applications for Identification of some Fruit Juices Flavonoids." Journal of Mass Spectrometry : JMS, vol. 44, no. 7, 2009, pp. 1017-25.
Abad-García B, Garmón-Lobato S, Berrueta LA, et al. Practical guidelines for characterization of O-diglycosyl flavonoid isomers by triple quadrupole MS and their applications for identification of some fruit juices flavonoids. J Mass Spectrom. 2009;44(7):1017-25.
Abad-García, B., Garmón-Lobato, S., Berrueta, L. A., Gallo, B., & Vicente, F. (2009). Practical guidelines for characterization of O-diglycosyl flavonoid isomers by triple quadrupole MS and their applications for identification of some fruit juices flavonoids. Journal of Mass Spectrometry : JMS, 44(7), 1017-25. https://doi.org/10.1002/jms.1573
Abad-García B, et al. Practical Guidelines for Characterization of O-diglycosyl Flavonoid Isomers By Triple Quadrupole MS and Their Applications for Identification of some Fruit Juices Flavonoids. J Mass Spectrom. 2009;44(7):1017-25. PubMed PMID: 19283783.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Practical guidelines for characterization of O-diglycosyl flavonoid isomers by triple quadrupole MS and their applications for identification of some fruit juices flavonoids. AU - Abad-García,B, AU - Garmón-Lobato,S, AU - Berrueta,L A, AU - Gallo,B, AU - Vicente,F, PY - 2009/3/14/entrez PY - 2009/3/14/pubmed PY - 2009/10/1/medline SP - 1017 EP - 25 JF - Journal of mass spectrometry : JMS JO - J Mass Spectrom VL - 44 IS - 7 N2 - Fifteen flavonoid O-diglycosides with different interglycosidic linkage isomery and glycosylation position have been studied in order to analyze their fragmentation patterns. Initial separation was carried out using high performance liquid chromatography with diode array detection (HPLC/DAD) coupled to an electrospray ionization (ESI) interface and a triple quadrupole mass spectrometer. Some useful differences in their MS spectra have been found and discussed. As it has already been reported, [Y*]+/[Y0]+ ratio for flavanones and [Y1]+/[Y0]+ ratio for other flavonoids is specific for each isomeric interglycosidic linkage. In this work it has also been observed that the abundance of these ions is dependent on the position of glycosylation. On the basis of these differences, systematic guidelines for our experimental conditions have been proposed for the differentiation of not only isomeric interglycosidic linkage but also glycosylation position using collision-induced dissociation MS/MS (CID-MS/MS) spectra in positive mode. These results have been successfully applied for the characterization of three diglycosyl flavonoids found in Citrus fruit juices and these conclusions have also been extrapolated for characterizing two triglycosides in the same fruits. SN - 1096-9888 UR - https://www.unboundmedicine.com/medline/citation/19283783/Practical_guidelines_for_characterization_of_O_diglycosyl_flavonoid_isomers_by_triple_quadrupole_MS_and_their_applications_for_identification_of_some_fruit_juices_flavonoids_ L2 - https://doi.org/10.1002/jms.1573 DB - PRIME DP - Unbound Medicine ER -