Tags

Type your tag names separated by a space and hit enter

Molecular detection of verotoxin-producing Escherichia coli isolates in stool samples from patients with diarrhea.
Roum Arch Microbiol Immunol. 2008 Jan-Jun; 67(1-2):17-22.RA

Abstract

Despite its occurence as a commensal in the human intestine, Escherichia coli is also known as a versatile gastrointestinal pathogen. Identification of diarrheagenic E. coli (DEC) requires the accurate discrimination of pathogenic strains from commensal flora, and this is not an easy task if the diagnostic tools are inadequate. As the information regarding the relative contribution of DEC among other identifiable causes of infectious diarrhea in Romanian patients is scarce, a prospective study was conducted to evaluate the prevalence of enteropathogenic Escherichia coli (EPEC) and verotoxin-producing Escherichia coli (VTEC) isolates in the diarrheagenic stool specimens of 120 children and 270 adults. PCR-based detection of the eae, bfp, vtx1 and vtx2 genes was added to the conventional culture and slide agglutination with 12 commercial EPEC antisera and O157:H7 antisera for identifying EPEC and VTEC isolates. Even though E. coli colonies belonging to traditional EPEC serogroups were isolated from 35 children and 17 adults, only the isolates recovered from 16 children and 2 adults harboured at least one of the targeted pathogenicity-associated genes. The children shedding EPEC outnumbered the adults (16.7% vs. 7.4%). Based on the virulence genotype identified, the prevalence of atypical EPEC (eae+) was higher than of typical EPEC (eae+ bfpA+), and typical EPEC identification was restricted to children. Owing to the molecular analysis 5 children and 10 adults that could have been overlooked by the routine microbiological investigation were diagnosed as infected with VTEC. Considering that none of the screened stool specimens was positive for E. coli O157:H7, this study reports for the first time the presence of VTEC nonO157 in local patients with diarrhea. Our results bring evidence that both EPEC and VTEC isolates are circulating as agents of local sporadic cases of human diarrhea. Further studies are needed to evaluate the contribution of DEC to the human disease burden in Romania, based on improved diagnostic tools targeting the main virulence traits of E. coli clinical isolates.

Authors+Show Affiliations

National Institute of Research-Development for Microbiology and Immunology Cantacuzino, Bucharest.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19284162

Citation

Usein, Codruţa-Romaniţa, et al. "Molecular Detection of Verotoxin-producing Escherichia Coli Isolates in Stool Samples From Patients With Diarrhea." Roumanian Archives of Microbiology and Immunology, vol. 67, no. 1-2, 2008, pp. 17-22.
Usein CR, Tatu-Chiţoiu D, Palade AM, et al. Molecular detection of verotoxin-producing Escherichia coli isolates in stool samples from patients with diarrhea. Roum Arch Microbiol Immunol. 2008;67(1-2):17-22.
Usein, C. R., Tatu-Chiţoiu, D., Palade, A. M., Ciontea, S., Condei, M., Nica, M., & Damian, M. (2008). Molecular detection of verotoxin-producing Escherichia coli isolates in stool samples from patients with diarrhea. Roumanian Archives of Microbiology and Immunology, 67(1-2), 17-22.
Usein CR, et al. Molecular Detection of Verotoxin-producing Escherichia Coli Isolates in Stool Samples From Patients With Diarrhea. Roum Arch Microbiol Immunol. 2008 Jan-Jun;67(1-2):17-22. PubMed PMID: 19284162.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular detection of verotoxin-producing Escherichia coli isolates in stool samples from patients with diarrhea. AU - Usein,Codruţa-Romaniţa, AU - Tatu-Chiţoiu,Dorina, AU - Palade,Andi-Marian, AU - Ciontea,Simona, AU - Condei,Maria, AU - Nica,Maria, AU - Damian,Maria, PY - 2009/3/17/entrez PY - 2008/1/1/pubmed PY - 2009/3/27/medline SP - 17 EP - 22 JF - Roumanian archives of microbiology and immunology JO - Roum Arch Microbiol Immunol VL - 67 IS - 1-2 N2 - Despite its occurence as a commensal in the human intestine, Escherichia coli is also known as a versatile gastrointestinal pathogen. Identification of diarrheagenic E. coli (DEC) requires the accurate discrimination of pathogenic strains from commensal flora, and this is not an easy task if the diagnostic tools are inadequate. As the information regarding the relative contribution of DEC among other identifiable causes of infectious diarrhea in Romanian patients is scarce, a prospective study was conducted to evaluate the prevalence of enteropathogenic Escherichia coli (EPEC) and verotoxin-producing Escherichia coli (VTEC) isolates in the diarrheagenic stool specimens of 120 children and 270 adults. PCR-based detection of the eae, bfp, vtx1 and vtx2 genes was added to the conventional culture and slide agglutination with 12 commercial EPEC antisera and O157:H7 antisera for identifying EPEC and VTEC isolates. Even though E. coli colonies belonging to traditional EPEC serogroups were isolated from 35 children and 17 adults, only the isolates recovered from 16 children and 2 adults harboured at least one of the targeted pathogenicity-associated genes. The children shedding EPEC outnumbered the adults (16.7% vs. 7.4%). Based on the virulence genotype identified, the prevalence of atypical EPEC (eae+) was higher than of typical EPEC (eae+ bfpA+), and typical EPEC identification was restricted to children. Owing to the molecular analysis 5 children and 10 adults that could have been overlooked by the routine microbiological investigation were diagnosed as infected with VTEC. Considering that none of the screened stool specimens was positive for E. coli O157:H7, this study reports for the first time the presence of VTEC nonO157 in local patients with diarrhea. Our results bring evidence that both EPEC and VTEC isolates are circulating as agents of local sporadic cases of human diarrhea. Further studies are needed to evaluate the contribution of DEC to the human disease burden in Romania, based on improved diagnostic tools targeting the main virulence traits of E. coli clinical isolates. SN - 1222-3891 UR - https://www.unboundmedicine.com/medline/citation/19284162/Molecular_detection_of_verotoxin_producing_Escherichia_coli_isolates_in_stool_samples_from_patients_with_diarrhea_ L2 - http://www.diseaseinfosearch.org/result/2258 DB - PRIME DP - Unbound Medicine ER -