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Oxymatrine protects rat brains against permanent focal ischemia and downregulates NF-kappaB expression.
Brain Res. 2009 May 01; 1268:174-180.BR

Abstract

BACKGROUND

Oxymatrine is proven to protect ischemic and reperfusion injury in liver, intestine and heart, this effect is via anti-inflammation and anti-apoptosis. Whether this protective effect applies to ischemic injury in brain, we therefore investigate the potential neuroprotective role of oxymatrine and the underlying mechanisms.

METHODS

Male, Sprague-Dawley rats were randomly assigned to four groups: permanent middle cerebral artery occlusion (pMCAO), high dose (pMCAO+oxymatrine 120 mg/kg), low dose (pMCAO+oxymatrine 60 mg/kg) and sham operated group. We used a permanent middle cerebral artery occlusion model and administered oxymatrine intraperitoneally immediately after cerebral ischemia and once daily on the following days. At 24 h after MCAO, neurological deficit was evaluated using a modified six point scale; brain water content was measured; NF-kappaB expression was measured by immunohistochemistry, Western blotting and RT-PCR. Infarct volume was analyzed with 2, 3, 5-triphenyltetrazolium chloride (TTC) staining at 72 h.

RESULTS

Compared with pMCAO group, neurological deficit in high dose group was improved (P<0.05), infarct volume was decreased (P<0.001) and cerebral edema was alleviated (P<0.05). Consistent with these indices, immunohistochemistry, Western blot and RT-PCR analysis indicated that NF-kappaB expression was significantly decreased in high dose group. Low dose of oxymatrine did not affect NF-kappaB expression in pMCAO rats.

CONCLUSIONS

Oxymatrine reduced infarct volume induced by pMCAO, this effect may be through the decreasing of NF-kappaB expression.

Authors+Show Affiliations

Department of Neurology, Second Hospital of Hebei Medical University. Shijiazhuang 050000, China.Department of Neurology, Second Hospital of Hebei Medical University. Shijiazhuang 050000, China. Electronic address: zhang6xj@heinfo.net.Department of Neurology, Second Hospital of Hebei Medical University. Shijiazhuang 050000, China.Department of Neurology, Second Hospital of Hebei Medical University. Shijiazhuang 050000, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19285049

Citation

Liu, Ying, et al. "Oxymatrine Protects Rat Brains Against Permanent Focal Ischemia and Downregulates NF-kappaB Expression." Brain Research, vol. 1268, 2009, pp. 174-180.
Liu Y, Zhang XJ, Yang CH, et al. Oxymatrine protects rat brains against permanent focal ischemia and downregulates NF-kappaB expression. Brain Res. 2009;1268:174-180.
Liu, Y., Zhang, X. J., Yang, C. H., & Fan, H. G. (2009). Oxymatrine protects rat brains against permanent focal ischemia and downregulates NF-kappaB expression. Brain Research, 1268, 174-180. https://doi.org/10.1016/j.brainres.2009.02.069
Liu Y, et al. Oxymatrine Protects Rat Brains Against Permanent Focal Ischemia and Downregulates NF-kappaB Expression. Brain Res. 2009 May 1;1268:174-180. PubMed PMID: 19285049.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oxymatrine protects rat brains against permanent focal ischemia and downregulates NF-kappaB expression. AU - Liu,Ying, AU - Zhang,Xiang-Jian, AU - Yang,Chen-Hui, AU - Fan,Hong-Guang, Y1 - 2009/03/10/ PY - 2008/12/12/received PY - 2009/02/15/revised PY - 2009/02/20/accepted PY - 2009/3/17/entrez PY - 2009/3/17/pubmed PY - 2009/6/25/medline SP - 174 EP - 180 JF - Brain research JO - Brain Res VL - 1268 N2 - BACKGROUND: Oxymatrine is proven to protect ischemic and reperfusion injury in liver, intestine and heart, this effect is via anti-inflammation and anti-apoptosis. Whether this protective effect applies to ischemic injury in brain, we therefore investigate the potential neuroprotective role of oxymatrine and the underlying mechanisms. METHODS: Male, Sprague-Dawley rats were randomly assigned to four groups: permanent middle cerebral artery occlusion (pMCAO), high dose (pMCAO+oxymatrine 120 mg/kg), low dose (pMCAO+oxymatrine 60 mg/kg) and sham operated group. We used a permanent middle cerebral artery occlusion model and administered oxymatrine intraperitoneally immediately after cerebral ischemia and once daily on the following days. At 24 h after MCAO, neurological deficit was evaluated using a modified six point scale; brain water content was measured; NF-kappaB expression was measured by immunohistochemistry, Western blotting and RT-PCR. Infarct volume was analyzed with 2, 3, 5-triphenyltetrazolium chloride (TTC) staining at 72 h. RESULTS: Compared with pMCAO group, neurological deficit in high dose group was improved (P<0.05), infarct volume was decreased (P<0.001) and cerebral edema was alleviated (P<0.05). Consistent with these indices, immunohistochemistry, Western blot and RT-PCR analysis indicated that NF-kappaB expression was significantly decreased in high dose group. Low dose of oxymatrine did not affect NF-kappaB expression in pMCAO rats. CONCLUSIONS: Oxymatrine reduced infarct volume induced by pMCAO, this effect may be through the decreasing of NF-kappaB expression. SN - 1872-6240 UR - https://www.unboundmedicine.com/medline/citation/19285049/Oxymatrine_protects_rat_brains_against_permanent_focal_ischemia_and_downregulates_NF_kappaB_expression_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-8993(09)00409-0 DB - PRIME DP - Unbound Medicine ER -