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Caffeic acid phenethyl ester inhibits osteoclastogenesis by suppressing NF kappaB and downregulating NFATc1 and c-Fos.
Int Immunopharmacol. 2009 Jun; 9(6):774-80.II

Abstract

Osteoclasts are multinuclear cells of myeloid lineage responsible for bone resorption. The anti-inflammatory property of caffeic acid phenethyl ester (CAPE), an active component of the propolis of honeybee hives, has been revealed. Since the regulatory mechanism of differentiation and activation of osteoclasts shares many well-known signaling pathways with that of inflammation, we investigated whether CAPE has any effect on osteoclastogenesis. CAPE potently suppressed osteoclastogenesis in cultures of bone marrow-derived precursor cells with the osteoclast differentiation factor, receptor activator of nuclear factor kappaB ligand (RANKL). While the RANKL-stimulated activation of the ERK, JNK, and p38 MAPK signaling pathways was not affected, the DNA binding and transcription activity of NF kappaB were reduced by CAPE treatment. In addition, CAPE blocked the induction of NFATc1 and c-Fos following RANKL stimulation. Forced expression of c-Fos could reverse the inhibitory effect of CAPE on osteoclastogenesis. Finally, CAPE significantly inhibited the RANKL-induced osteoclast formation in mouse calvariae in vivo. We propose that CAPE might be useful as a therapeutic agent for treatment of bone destructive diseases.

Authors+Show Affiliations

Department of Cell and Developmental Biology, BK21 Program and Dental Research Institute, Seoul National University, 28 Yeongon-Dong, Chongno-Gu, Seoul 110-749, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19285574

Citation

Ha, Jeongim, et al. "Caffeic Acid Phenethyl Ester Inhibits Osteoclastogenesis By Suppressing NF kappaB and Downregulating NFATc1 and C-Fos." International Immunopharmacology, vol. 9, no. 6, 2009, pp. 774-80.
Ha J, Choi HS, Lee Y, et al. Caffeic acid phenethyl ester inhibits osteoclastogenesis by suppressing NF kappaB and downregulating NFATc1 and c-Fos. Int Immunopharmacol. 2009;9(6):774-80.
Ha, J., Choi, H. S., Lee, Y., Lee, Z. H., & Kim, H. H. (2009). Caffeic acid phenethyl ester inhibits osteoclastogenesis by suppressing NF kappaB and downregulating NFATc1 and c-Fos. International Immunopharmacology, 9(6), 774-80. https://doi.org/10.1016/j.intimp.2009.03.001
Ha J, et al. Caffeic Acid Phenethyl Ester Inhibits Osteoclastogenesis By Suppressing NF kappaB and Downregulating NFATc1 and C-Fos. Int Immunopharmacol. 2009;9(6):774-80. PubMed PMID: 19285574.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Caffeic acid phenethyl ester inhibits osteoclastogenesis by suppressing NF kappaB and downregulating NFATc1 and c-Fos. AU - Ha,Jeongim, AU - Choi,Hyo-Sun, AU - Lee,Youngkyun, AU - Lee,Zang Hee, AU - Kim,Hong-Hee, Y1 - 2009/03/12/ PY - 2009/01/31/received PY - 2009/03/02/revised PY - 2009/03/02/accepted PY - 2009/3/17/entrez PY - 2009/3/17/pubmed PY - 2009/8/18/medline SP - 774 EP - 80 JF - International immunopharmacology JO - Int Immunopharmacol VL - 9 IS - 6 N2 - Osteoclasts are multinuclear cells of myeloid lineage responsible for bone resorption. The anti-inflammatory property of caffeic acid phenethyl ester (CAPE), an active component of the propolis of honeybee hives, has been revealed. Since the regulatory mechanism of differentiation and activation of osteoclasts shares many well-known signaling pathways with that of inflammation, we investigated whether CAPE has any effect on osteoclastogenesis. CAPE potently suppressed osteoclastogenesis in cultures of bone marrow-derived precursor cells with the osteoclast differentiation factor, receptor activator of nuclear factor kappaB ligand (RANKL). While the RANKL-stimulated activation of the ERK, JNK, and p38 MAPK signaling pathways was not affected, the DNA binding and transcription activity of NF kappaB were reduced by CAPE treatment. In addition, CAPE blocked the induction of NFATc1 and c-Fos following RANKL stimulation. Forced expression of c-Fos could reverse the inhibitory effect of CAPE on osteoclastogenesis. Finally, CAPE significantly inhibited the RANKL-induced osteoclast formation in mouse calvariae in vivo. We propose that CAPE might be useful as a therapeutic agent for treatment of bone destructive diseases. SN - 1878-1705 UR - https://www.unboundmedicine.com/medline/citation/19285574/Caffeic_acid_phenethyl_ester_inhibits_osteoclastogenesis_by_suppressing_NF_kappaB_and_downregulating_NFATc1_and_c_Fos_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-5769(09)00092-7 DB - PRIME DP - Unbound Medicine ER -