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Determination of duloxetine in human plasma by capillary electrophoresis with laser-induced fluorescence detection.
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Apr 15; 877(11-12):1126-32.JC

Abstract

A method based on capillary electrophoresis has been developed for the analysis of the novel antidepressant drug duloxetine in human plasma. The method makes use of laser-induced fluorescence detection after derivatisation of the analyte with 5-(4,6-dichlorotriazinyl)aminofluorescein at pH 11. A single step liquid/liquid extraction procedure with a mixture of hexane/2-propanol allows the sample clean-up with extraction yields always >or=84% and interference removal. The electrophoretic separation is achieved using uncoated fused silica capillaries (60.0 cm effective length, 75.0 cm total length, 50 microm internal diameter) and a background electrolyte composed of borate buffer (40 mM, pH 10.3), tetrabutylammonium bromide (10 mM), and acetone (10%, v/v). The applied voltage is 20 kV; the samples are injected by pressure (50 mbar x 8 s). The method has been fully validated in terms of linearity range (2.5-150 ng mL(-1)), LOD and LOQ (1.0 and 2.5 ng mL(-1), respectively), precision (R.S.D.<6.7%) and accuracy (recovery >78%). Application to samples obtained from patients under treatment with duloxetine gave good results. The method represents the first application of capillary electrophoresis to the analysis of duloxetine in human plasma.

Authors+Show Affiliations

Department of Pharmaceutical Sciences, Alma Mater Studiorum-University of Bologna, Bologna, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19285923

Citation

Musenga, Alessandro, et al. "Determination of Duloxetine in Human Plasma By Capillary Electrophoresis With Laser-induced Fluorescence Detection." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 877, no. 11-12, 2009, pp. 1126-32.
Musenga A, Amore M, Mandrioli R, et al. Determination of duloxetine in human plasma by capillary electrophoresis with laser-induced fluorescence detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2009;877(11-12):1126-32.
Musenga, A., Amore, M., Mandrioli, R., Kenndler, E., de Martino, L., & Raggi, M. A. (2009). Determination of duloxetine in human plasma by capillary electrophoresis with laser-induced fluorescence detection. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 877(11-12), 1126-32. https://doi.org/10.1016/j.jchromb.2009.02.059
Musenga A, et al. Determination of Duloxetine in Human Plasma By Capillary Electrophoresis With Laser-induced Fluorescence Detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Apr 15;877(11-12):1126-32. PubMed PMID: 19285923.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of duloxetine in human plasma by capillary electrophoresis with laser-induced fluorescence detection. AU - Musenga,Alessandro, AU - Amore,Mario, AU - Mandrioli,Roberto, AU - Kenndler,Ernst, AU - de Martino,Ludovica, AU - Raggi,Maria Augusta, Y1 - 2009/03/03/ PY - 2008/12/23/received PY - 2009/02/24/revised PY - 2009/02/25/accepted PY - 2009/3/17/entrez PY - 2009/3/17/pubmed PY - 2009/5/9/medline SP - 1126 EP - 32 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 877 IS - 11-12 N2 - A method based on capillary electrophoresis has been developed for the analysis of the novel antidepressant drug duloxetine in human plasma. The method makes use of laser-induced fluorescence detection after derivatisation of the analyte with 5-(4,6-dichlorotriazinyl)aminofluorescein at pH 11. A single step liquid/liquid extraction procedure with a mixture of hexane/2-propanol allows the sample clean-up with extraction yields always >or=84% and interference removal. The electrophoretic separation is achieved using uncoated fused silica capillaries (60.0 cm effective length, 75.0 cm total length, 50 microm internal diameter) and a background electrolyte composed of borate buffer (40 mM, pH 10.3), tetrabutylammonium bromide (10 mM), and acetone (10%, v/v). The applied voltage is 20 kV; the samples are injected by pressure (50 mbar x 8 s). The method has been fully validated in terms of linearity range (2.5-150 ng mL(-1)), LOD and LOQ (1.0 and 2.5 ng mL(-1), respectively), precision (R.S.D.<6.7%) and accuracy (recovery >78%). Application to samples obtained from patients under treatment with duloxetine gave good results. The method represents the first application of capillary electrophoresis to the analysis of duloxetine in human plasma. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/19285923/Determination_of_duloxetine_in_human_plasma_by_capillary_electrophoresis_with_laser_induced_fluorescence_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(09)00159-7 DB - PRIME DP - Unbound Medicine ER -