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Curcumin inhibits proliferation and migration by increasing the Bax to Bcl-2 ratio and decreasing NF-kappaBp65 expression in breast cancer MDA-MB-231 cells.
Int J Mol Med. 2009 Apr; 23(4):469-75.IJ

Abstract

Curcumin (1,7-bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione), is extracted from the plant Curcuma longa. It has cytotoxic effects and induces apoptosis in many human cancer cells but the molecular mechanisms are not fully understood. In the present study, we evaluated the effects of curcumin on human breast cancer MDA-MB-231 cells. The cytotoxic effects of curcumin on MDA-MB-231 cells were measured by MTT assay. The percentages of cell cycle were determined by flow cytometry. The protein expressions of p21, 53, Bax and Bcl-2 were examined by Western blotting. The results show that curcumin inhibits the proliferation of MDA-MB-231 cells and induces G2/M arrest in a dose-dependent manner. Curcumin increased the protein expressions of p21 and Bax, but decreased the protein expression of p53 and Bcl-2 in MDA-MB-231 cells. Our results show that one molecular mechanism of curcumin inhibits the proliferation of MDA-MB-231 cells either through up-regulating p21 expression and then inducing apoptosis, or through up-regulating the Bax to Bcl-2 ratio and then inducing apoptosis. Our results also show that curcumin inhibits the migratory activity of MDA-MB-231 cells through down-regulating the protein expression of NF-kappaBp65. Accordingly, the therapeutic potential of curcumin for breast cancer deserves further study.

Authors+Show Affiliations

Institute of Medical Sciences, Tzu-Chi University, and Division of Neuro-Oncology, Neuro-Medical Scientific Center, Buddhist Tzu Chi General Hospital, Hualien, Hualien City 970, Taiwan, ROC.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19288022

Citation

Chiu, Tsung-Lang, and Chin-Cheng Su. "Curcumin Inhibits Proliferation and Migration By Increasing the Bax to Bcl-2 Ratio and Decreasing NF-kappaBp65 Expression in Breast Cancer MDA-MB-231 Cells." International Journal of Molecular Medicine, vol. 23, no. 4, 2009, pp. 469-75.
Chiu TL, Su CC. Curcumin inhibits proliferation and migration by increasing the Bax to Bcl-2 ratio and decreasing NF-kappaBp65 expression in breast cancer MDA-MB-231 cells. Int J Mol Med. 2009;23(4):469-75.
Chiu, T. L., & Su, C. C. (2009). Curcumin inhibits proliferation and migration by increasing the Bax to Bcl-2 ratio and decreasing NF-kappaBp65 expression in breast cancer MDA-MB-231 cells. International Journal of Molecular Medicine, 23(4), 469-75.
Chiu TL, Su CC. Curcumin Inhibits Proliferation and Migration By Increasing the Bax to Bcl-2 Ratio and Decreasing NF-kappaBp65 Expression in Breast Cancer MDA-MB-231 Cells. Int J Mol Med. 2009;23(4):469-75. PubMed PMID: 19288022.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Curcumin inhibits proliferation and migration by increasing the Bax to Bcl-2 ratio and decreasing NF-kappaBp65 expression in breast cancer MDA-MB-231 cells. AU - Chiu,Tsung-Lang, AU - Su,Chin-Cheng, PY - 2009/3/17/entrez PY - 2009/3/17/pubmed PY - 2009/8/8/medline SP - 469 EP - 75 JF - International journal of molecular medicine JO - Int J Mol Med VL - 23 IS - 4 N2 - Curcumin (1,7-bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione), is extracted from the plant Curcuma longa. It has cytotoxic effects and induces apoptosis in many human cancer cells but the molecular mechanisms are not fully understood. In the present study, we evaluated the effects of curcumin on human breast cancer MDA-MB-231 cells. The cytotoxic effects of curcumin on MDA-MB-231 cells were measured by MTT assay. The percentages of cell cycle were determined by flow cytometry. The protein expressions of p21, 53, Bax and Bcl-2 were examined by Western blotting. The results show that curcumin inhibits the proliferation of MDA-MB-231 cells and induces G2/M arrest in a dose-dependent manner. Curcumin increased the protein expressions of p21 and Bax, but decreased the protein expression of p53 and Bcl-2 in MDA-MB-231 cells. Our results show that one molecular mechanism of curcumin inhibits the proliferation of MDA-MB-231 cells either through up-regulating p21 expression and then inducing apoptosis, or through up-regulating the Bax to Bcl-2 ratio and then inducing apoptosis. Our results also show that curcumin inhibits the migratory activity of MDA-MB-231 cells through down-regulating the protein expression of NF-kappaBp65. Accordingly, the therapeutic potential of curcumin for breast cancer deserves further study. SN - 1107-3756 UR - https://www.unboundmedicine.com/medline/citation/19288022/Curcumin_inhibits_proliferation_and_migration_by_increasing_the_Bax_to_Bcl_2_ratio_and_decreasing_NF_kappaBp65_expression_in_breast_cancer_MDA_MB_231_cells_ L2 - http://www.spandidos-publications.com/ijmm/23/4/469 DB - PRIME DP - Unbound Medicine ER -