Tags

Type your tag names separated by a space and hit enter

[Effect of electroacupuncture on apoptosis of hippocampus tissue in mice with vascular dementia].
Zhen Ci Yan Jiu. 2008 Dec; 33(6):377-81.ZC

Abstract

OBJECTIVE

To observe the effect of electroacupuncture (EA) on hippocampal apoptosis and learning-memory ability in vascular dementia (VD) mice so as to investigate its underlying mechanism in the treatment of VD patients.

METHODS

Kunming mice were randomly divided into sham-operation (sham, n = 13), model (n = 15), EA (n = 16) and Nimodipine (intragastric gavage, 30 mg/kg for 15 days, n = 15) groups. VD model was duplicated by occlusion of bilateral carotid arteries and reperfusion. EA (2-80 Hz, 2 mA) was applied to "Dazhui" (GV 14), "Baihui" (GV 20), "Geshu" (BL 17) and "Zusanli" (ST 36) for 10 min, once daily for 15 days. Step-down and step-up tests were performed to assess the animal's memory and learning abilities separately; and the terminal deoxynucleotidyl transferase-mediated dUTP-nick end-labeling (TUNEL) method was used to display the apoptotic cells of the hippocampus tissue.

RESULTS

In comparison with sham group, the animals' reaction time upon electric shock stimulation, both step-up and step-down error times, and hippocampal apoptosis number in model, EA and Nimodipine groups increased significantly (P<0.01), while the reaction latency upon electric shock of model, EA and Nimodipine groups decreased significantly (P<0.01). Compared with model group, the reaction time, both step-up and step-down error times and hippocampal apoptosis number of both EA and Nimodipine groups decreased significantly (P<0.05, P<0.01); while the reaction latency of EA and Nimodipine groups increased considerably (P<0.01). Comparison between EA and Nimodipine groups showed that the reaction latency of EA group was obviously longer than that of the later group (P<0.01), and the step-down error times of EA group was markedly lower than that of Nimodipine group (P<0.05). No significant differences were found between these two groups in other indexes (P>0.05).

CONCLUSION

EA can ameliorate VD mice's learning-memory ability, which may be closely related to its effect in reducing hippocampal apoptosis.

Authors+Show Affiliations

College of Chinese Medicine, Hebei Medical University, Shijiazhuang, 050091, China. zhz6212202@163.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

19288897

Citation

Zhang, Hui-zhen, et al. "[Effect of Electroacupuncture On Apoptosis of Hippocampus Tissue in Mice With Vascular Dementia]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 33, no. 6, 2008, pp. 377-81.
Zhang HZ, Zhang LX, She YF, et al. [Effect of electroacupuncture on apoptosis of hippocampus tissue in mice with vascular dementia]. Zhen Ci Yan Jiu. 2008;33(6):377-81.
Zhang, H. Z., Zhang, L. X., She, Y. F., Li, W. L., Zhang, X. J., & Zhang, R. J. (2008). [Effect of electroacupuncture on apoptosis of hippocampus tissue in mice with vascular dementia]. Zhen Ci Yan Jiu = Acupuncture Research, 33(6), 377-81.
Zhang HZ, et al. [Effect of Electroacupuncture On Apoptosis of Hippocampus Tissue in Mice With Vascular Dementia]. Zhen Ci Yan Jiu. 2008;33(6):377-81. PubMed PMID: 19288897.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of electroacupuncture on apoptosis of hippocampus tissue in mice with vascular dementia]. AU - Zhang,Hui-zhen, AU - Zhang,Li-xin, AU - She,Yan-fen, AU - Li,Wen-li, AU - Zhang,Xue-jing, AU - Zhang,Rui-juan, PY - 2009/3/18/entrez PY - 2009/3/18/pubmed PY - 2009/4/10/medline SP - 377 EP - 81 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 33 IS - 6 N2 - OBJECTIVE: To observe the effect of electroacupuncture (EA) on hippocampal apoptosis and learning-memory ability in vascular dementia (VD) mice so as to investigate its underlying mechanism in the treatment of VD patients. METHODS: Kunming mice were randomly divided into sham-operation (sham, n = 13), model (n = 15), EA (n = 16) and Nimodipine (intragastric gavage, 30 mg/kg for 15 days, n = 15) groups. VD model was duplicated by occlusion of bilateral carotid arteries and reperfusion. EA (2-80 Hz, 2 mA) was applied to "Dazhui" (GV 14), "Baihui" (GV 20), "Geshu" (BL 17) and "Zusanli" (ST 36) for 10 min, once daily for 15 days. Step-down and step-up tests were performed to assess the animal's memory and learning abilities separately; and the terminal deoxynucleotidyl transferase-mediated dUTP-nick end-labeling (TUNEL) method was used to display the apoptotic cells of the hippocampus tissue. RESULTS: In comparison with sham group, the animals' reaction time upon electric shock stimulation, both step-up and step-down error times, and hippocampal apoptosis number in model, EA and Nimodipine groups increased significantly (P<0.01), while the reaction latency upon electric shock of model, EA and Nimodipine groups decreased significantly (P<0.01). Compared with model group, the reaction time, both step-up and step-down error times and hippocampal apoptosis number of both EA and Nimodipine groups decreased significantly (P<0.05, P<0.01); while the reaction latency of EA and Nimodipine groups increased considerably (P<0.01). Comparison between EA and Nimodipine groups showed that the reaction latency of EA group was obviously longer than that of the later group (P<0.01), and the step-down error times of EA group was markedly lower than that of Nimodipine group (P<0.05). No significant differences were found between these two groups in other indexes (P>0.05). CONCLUSION: EA can ameliorate VD mice's learning-memory ability, which may be closely related to its effect in reducing hippocampal apoptosis. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/19288897/[Effect_of_electroacupuncture_on_apoptosis_of_hippocampus_tissue_in_mice_with_vascular_dementia]_ DB - PRIME DP - Unbound Medicine ER -