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Effects of dietary N-acetylcysteine on the oxidative stress induced in tilapia (Oreochromis niloticus) exposed to a microcystin-producing cyanobacterial water bloom.
Environ Toxicol Chem. 2009 Aug; 28(8):1679-86.ET

Abstract

Fish can be exposed to toxic cyanobacterial cells in natural waters and fish farms and suffer from oxidative damage. The present study investigates the effects of N-acetylcysteine (NAC), a glutathione (GSH) precursor, on the oxidative stress induced by Microcystis cyanobacterial cells containing microcystins (MCs) in tilapia fish (Oreochromis niloticus). Variation in lipid peroxidation (LPO) levels, carbonyl group content, reduced glutathione to oxidized glutathione ratio (GSH:GSSG), and catalase (Enzyme Commission [EC] 1.11.1.6), superoxide dismutase (SOD; EC 1.15.1.1), glutathione reductase (GR; EC 1.8.1.7), glutathione peroxidase (GPx; EC 1.11.1.9), and glutathione S-transferase (EC 2.5.1.18) activities in liver and kidney of tilapia exposed to a single oral dose of 120 microg MC-LR (with leucine [L] and arginine [R])/fish and killed in 24 h were investigated in the absence and presence of 20.0, 44.0, and 96.8 mg NAC/fish/d. Results showed a protective role of NAC, depending on the dose and the biomarker considered. The increase in LPO (1.9- and 1.4-fold in liver and kidney, respectively) and the decreased protein content and GSH: GSSG in the liver induced by MCs were recovered mainly by the lower doses of NAC employed. Antioxidant enzyme activities increased (range, 1.4- to 1.7-fold) by MCs also were ameliorated by NAC, although the highest level used induced significant alteration of some enzymatic activities, such as SOD, GPx, and GR. Thus, NAC can be considered to be a useful chemoprotectant that reduces hepatic and renal oxidative stress in the prophylaxis and treatment of MC-related intoxications in fish when careful attention is given to its application dose because of its own pro-oxidant activity, as shown in the present study at 96.8 mg NAC/fish/d.

Authors+Show Affiliations

Area of Toxicology, Faculty of Pharmacy, University of Seville, Profesor García González Street Number 2, Seville 41012, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19290682

Citation

Puerto, María, et al. "Effects of Dietary N-acetylcysteine On the Oxidative Stress Induced in Tilapia (Oreochromis Niloticus) Exposed to a Microcystin-producing Cyanobacterial Water Bloom." Environmental Toxicology and Chemistry, vol. 28, no. 8, 2009, pp. 1679-86.
Puerto M, Prieto AI, Pichardo S, et al. Effects of dietary N-acetylcysteine on the oxidative stress induced in tilapia (Oreochromis niloticus) exposed to a microcystin-producing cyanobacterial water bloom. Environ Toxicol Chem. 2009;28(8):1679-86.
Puerto, M., Prieto, A. I., Pichardo, S., Moreno, I., Jos, A., Moyano, R., & Cameán, A. M. (2009). Effects of dietary N-acetylcysteine on the oxidative stress induced in tilapia (Oreochromis niloticus) exposed to a microcystin-producing cyanobacterial water bloom. Environmental Toxicology and Chemistry, 28(8), 1679-86. https://doi.org/10.1897/08-520.1
Puerto M, et al. Effects of Dietary N-acetylcysteine On the Oxidative Stress Induced in Tilapia (Oreochromis Niloticus) Exposed to a Microcystin-producing Cyanobacterial Water Bloom. Environ Toxicol Chem. 2009;28(8):1679-86. PubMed PMID: 19290682.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of dietary N-acetylcysteine on the oxidative stress induced in tilapia (Oreochromis niloticus) exposed to a microcystin-producing cyanobacterial water bloom. AU - Puerto,María, AU - Prieto,Ana I, AU - Pichardo,Silvia, AU - Moreno,Isabel, AU - Jos,Angeles, AU - Moyano,Rosario, AU - Cameán,Ana M, Y1 - 2009/03/16/ PY - 2008/10/16/received PY - 2009/02/01/accepted PY - 2009/3/18/entrez PY - 2009/3/18/pubmed PY - 2009/9/18/medline SP - 1679 EP - 86 JF - Environmental toxicology and chemistry JO - Environ Toxicol Chem VL - 28 IS - 8 N2 - Fish can be exposed to toxic cyanobacterial cells in natural waters and fish farms and suffer from oxidative damage. The present study investigates the effects of N-acetylcysteine (NAC), a glutathione (GSH) precursor, on the oxidative stress induced by Microcystis cyanobacterial cells containing microcystins (MCs) in tilapia fish (Oreochromis niloticus). Variation in lipid peroxidation (LPO) levels, carbonyl group content, reduced glutathione to oxidized glutathione ratio (GSH:GSSG), and catalase (Enzyme Commission [EC] 1.11.1.6), superoxide dismutase (SOD; EC 1.15.1.1), glutathione reductase (GR; EC 1.8.1.7), glutathione peroxidase (GPx; EC 1.11.1.9), and glutathione S-transferase (EC 2.5.1.18) activities in liver and kidney of tilapia exposed to a single oral dose of 120 microg MC-LR (with leucine [L] and arginine [R])/fish and killed in 24 h were investigated in the absence and presence of 20.0, 44.0, and 96.8 mg NAC/fish/d. Results showed a protective role of NAC, depending on the dose and the biomarker considered. The increase in LPO (1.9- and 1.4-fold in liver and kidney, respectively) and the decreased protein content and GSH: GSSG in the liver induced by MCs were recovered mainly by the lower doses of NAC employed. Antioxidant enzyme activities increased (range, 1.4- to 1.7-fold) by MCs also were ameliorated by NAC, although the highest level used induced significant alteration of some enzymatic activities, such as SOD, GPx, and GR. Thus, NAC can be considered to be a useful chemoprotectant that reduces hepatic and renal oxidative stress in the prophylaxis and treatment of MC-related intoxications in fish when careful attention is given to its application dose because of its own pro-oxidant activity, as shown in the present study at 96.8 mg NAC/fish/d. SN - 0730-7268 UR - https://www.unboundmedicine.com/medline/citation/19290682/Effects_of_dietary_N_acetylcysteine_on_the_oxidative_stress_induced_in_tilapia__Oreochromis_niloticus__exposed_to_a_microcystin_producing_cyanobacterial_water_bloom_ L2 - https://doi.org/10.1897/08-520.1 DB - PRIME DP - Unbound Medicine ER -