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Preparation, characterization, and oral delivery of insulin loaded carboxylated chitosan grafted poly(methyl methacrylate) nanoparticles.
Biomacromolecules. 2009 May 11; 10(5):1253-8.B

Abstract

To improve the efficiency of insulin via oral administration, pH-sensitive carboxylated chitosan grafted poly(methyl methacrylate) nanoparticles (CCGN) were prepared. CCGN were characterized by (1)H NMR, dynamic light scattering, zeta potential, and transmission electron microscopy, and the hypoglycemic effect of insulin loaded CCGN via the oral route was evaluated in normal and diabetic rats. CCGN exhibited a homogeneous morphology and a spherical shape with core-shell structure. They were aggregated in simulated gastric fluid while separated in simulated intestinal fluid. Insulin was mainly located in the shell of the CCGN via hydrogen bonding, electrostatic interaction, and Van der Waals force. Insulin release from the CCGN exhibited a pH-sensitive property in that it had a slow release rate at pH 2.0 and a fast release rate at pH 6.8 and 7.4. The pharmacological bioavailability after oral administration of insulin loaded CCGN at a dose of 25 IU/kg was found to be 9.7%. Besides, CCGN showed desirable tissue and blood compatibility. Therefore, the CCGN would be a promising delivery carrier for protein drugs via the oral route.

Authors+Show Affiliations

State Key Laboratory of Genetic Engineering, Department of Pharmaceutical Sciences, School of Life Sciences, Fudan University, Shanghai 200433, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19292439

Citation

Cui, Fuying, et al. "Preparation, Characterization, and Oral Delivery of Insulin Loaded Carboxylated Chitosan Grafted Poly(methyl Methacrylate) Nanoparticles." Biomacromolecules, vol. 10, no. 5, 2009, pp. 1253-8.
Cui F, Qian F, Zhao Z, et al. Preparation, characterization, and oral delivery of insulin loaded carboxylated chitosan grafted poly(methyl methacrylate) nanoparticles. Biomacromolecules. 2009;10(5):1253-8.
Cui, F., Qian, F., Zhao, Z., Yin, L., Tang, C., & Yin, C. (2009). Preparation, characterization, and oral delivery of insulin loaded carboxylated chitosan grafted poly(methyl methacrylate) nanoparticles. Biomacromolecules, 10(5), 1253-8. https://doi.org/10.1021/bm900035u
Cui F, et al. Preparation, Characterization, and Oral Delivery of Insulin Loaded Carboxylated Chitosan Grafted Poly(methyl Methacrylate) Nanoparticles. Biomacromolecules. 2009 May 11;10(5):1253-8. PubMed PMID: 19292439.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preparation, characterization, and oral delivery of insulin loaded carboxylated chitosan grafted poly(methyl methacrylate) nanoparticles. AU - Cui,Fuying, AU - Qian,Feng, AU - Zhao,Ziming, AU - Yin,Lichen, AU - Tang,Cui, AU - Yin,Chunhua, PY - 2009/3/19/entrez PY - 2009/3/19/pubmed PY - 2009/8/18/medline SP - 1253 EP - 8 JF - Biomacromolecules JO - Biomacromolecules VL - 10 IS - 5 N2 - To improve the efficiency of insulin via oral administration, pH-sensitive carboxylated chitosan grafted poly(methyl methacrylate) nanoparticles (CCGN) were prepared. CCGN were characterized by (1)H NMR, dynamic light scattering, zeta potential, and transmission electron microscopy, and the hypoglycemic effect of insulin loaded CCGN via the oral route was evaluated in normal and diabetic rats. CCGN exhibited a homogeneous morphology and a spherical shape with core-shell structure. They were aggregated in simulated gastric fluid while separated in simulated intestinal fluid. Insulin was mainly located in the shell of the CCGN via hydrogen bonding, electrostatic interaction, and Van der Waals force. Insulin release from the CCGN exhibited a pH-sensitive property in that it had a slow release rate at pH 2.0 and a fast release rate at pH 6.8 and 7.4. The pharmacological bioavailability after oral administration of insulin loaded CCGN at a dose of 25 IU/kg was found to be 9.7%. Besides, CCGN showed desirable tissue and blood compatibility. Therefore, the CCGN would be a promising delivery carrier for protein drugs via the oral route. SN - 1526-4602 UR - https://www.unboundmedicine.com/medline/citation/19292439/Preparation_characterization_and_oral_delivery_of_insulin_loaded_carboxylated_chitosan_grafted_poly_methyl_methacrylate__nanoparticles_ DB - PRIME DP - Unbound Medicine ER -