Tags

Type your tag names separated by a space and hit enter

Reflectance confocal microscopy for the in vivo detection of Treponema pallidum in skin lesions of secondary syphilis.
J Am Acad Dermatol. 2009 Apr; 60(4):639-42.JA

Abstract

BACKGROUND

Secondary syphilis is a diagnostic challenge that relies on microscopic and laboratory tests.

OBJECTIVE

We sought to assess the usefulness of in vivo reflectance confocal microscopy (RCM) to detect Treponema pallidum in lesions suggestive of secondary syphilis.

METHODS

Macular and papular skin lesions from 3 patients clinically suggestive of secondary syphilis were imaged by RCM and confirmed by skin punch biopsy.

RESULTS

In all lesions RCM demonstrated elongated small bright particles with a spiral shape intermingled with the keratinocytes. These features corresponded with immunohistochemical findings that revealed several spirochetes infiltrating the epidermis.

LIMITATIONS

Unlike immunohistochemistry, RCM did not visualize T pallidum in the dermis and vascular walls because of limited imaging depth. The specificity and sensitivity of this technique need to be assessed.

CONCLUSION

RCM may be an effective diagnostic tool for in vivo real-time imaging of T pallidum in skin lesions of secondary syphilis, and seems to correlate well with immunohistochemistry.

Authors+Show Affiliations

Department of Dermatology, University of Brescia, Spedali Civili Hospital, Brescia, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19293012

Citation

Venturini, Marina, et al. "Reflectance Confocal Microscopy for the in Vivo Detection of Treponema Pallidum in Skin Lesions of Secondary Syphilis." Journal of the American Academy of Dermatology, vol. 60, no. 4, 2009, pp. 639-42.
Venturini M, Sala R, Semenza D, et al. Reflectance confocal microscopy for the in vivo detection of Treponema pallidum in skin lesions of secondary syphilis. J Am Acad Dermatol. 2009;60(4):639-42.
Venturini, M., Sala, R., Semenza, D., Santoro, A., Facchetti, F., & Calzavara-Pinton, P. (2009). Reflectance confocal microscopy for the in vivo detection of Treponema pallidum in skin lesions of secondary syphilis. Journal of the American Academy of Dermatology, 60(4), 639-42. https://doi.org/10.1016/j.jaad.2008.11.901
Venturini M, et al. Reflectance Confocal Microscopy for the in Vivo Detection of Treponema Pallidum in Skin Lesions of Secondary Syphilis. J Am Acad Dermatol. 2009;60(4):639-42. PubMed PMID: 19293012.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Reflectance confocal microscopy for the in vivo detection of Treponema pallidum in skin lesions of secondary syphilis. AU - Venturini,Marina, AU - Sala,Raffaella, AU - Semenza,Diego, AU - Santoro,Amerigo, AU - Facchetti,Fabio, AU - Calzavara-Pinton,Piergiacomo, PY - 2008/10/07/received PY - 2008/11/17/revised PY - 2008/11/17/accepted PY - 2009/3/19/entrez PY - 2009/3/19/pubmed PY - 2009/4/18/medline SP - 639 EP - 42 JF - Journal of the American Academy of Dermatology JO - J Am Acad Dermatol VL - 60 IS - 4 N2 - BACKGROUND: Secondary syphilis is a diagnostic challenge that relies on microscopic and laboratory tests. OBJECTIVE: We sought to assess the usefulness of in vivo reflectance confocal microscopy (RCM) to detect Treponema pallidum in lesions suggestive of secondary syphilis. METHODS: Macular and papular skin lesions from 3 patients clinically suggestive of secondary syphilis were imaged by RCM and confirmed by skin punch biopsy. RESULTS: In all lesions RCM demonstrated elongated small bright particles with a spiral shape intermingled with the keratinocytes. These features corresponded with immunohistochemical findings that revealed several spirochetes infiltrating the epidermis. LIMITATIONS: Unlike immunohistochemistry, RCM did not visualize T pallidum in the dermis and vascular walls because of limited imaging depth. The specificity and sensitivity of this technique need to be assessed. CONCLUSION: RCM may be an effective diagnostic tool for in vivo real-time imaging of T pallidum in skin lesions of secondary syphilis, and seems to correlate well with immunohistochemistry. SN - 1097-6787 UR - https://www.unboundmedicine.com/medline/citation/19293012/Reflectance_confocal_microscopy_for_the_in_vivo_detection_of_Treponema_pallidum_in_skin_lesions_of_secondary_syphilis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0190-9622(08)02475-4 DB - PRIME DP - Unbound Medicine ER -