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Immunogencity of HSA-L7/L12 (Brucella abortus ribosomal protein) in an animal model.
Iran J Immunol 2009; 6(1):12-21IJ

Abstract

BACKGROUND

The immunogenic Brucella abortus ribosomal protein L7/L12 is a promising candidate antigen for the development of subunit vaccines against brucellosis.

OBJECTIVE

This study was aimed to evaluate the protection of recombinant Human Serum Albumin (HAS)-L7/L12 fusion protein in Balb/c mice.

METHODS

The amplified L7/L12 gene was cloned in pYHSA5 vector, pYHSA5-L7/L12 construct was transformed in Saccharomyces cerevisiae and the expressed protein from supernatant was purified by affinity chromatography. Balb/c mice were immunized in five groups by tHSA-L7/L12 fusion protein (group 1), Brucella abortus S19 (group 2), HSA (group 3), recombinant L7/L12 (group 4), PBS (group 5). ELISA to detect antibody production, LTT test to assess antigen specific lymphocyte response were conducted prior to virulent B. abortus strain 544 challenge two weeks after the last injection. Bacterial counts from spleens of immunized mice were done four weeks after challenge.

RESULTS

In ELISA tests, the specific antibodies exhibited a dominance of immunoglobulin IgG1 over IgG2a. In addition, the tHSA-L7/L12 fusion protein and L7/L12 elicited a strong T-cell proliferative response upon restimulation in vitro with recombinant tHSA-L7/L12 and L7/L12, suggesting the induction of a cellular immunity response in vivo. However, there was no significant difference in proliferative response of L7/L12 and tHSA-L7/L12 fusion protein (p>0.05). The L7/L12 and tHSA-L7/L12 fusion protein vaccines could also induce significant protection against challenge with the virulent strain B. abortus 544 in Balb/c mice (p< or =0.05).

CONCLUSION

The tHSA-L7/L12 fusion protein, similar to L7/L12 has the ability to induce antigen specific lymphocyte proliferation, stimulate humoral immunity and engender protection.

Authors+Show Affiliations

Department of Microbiology, Ilam University of Medical Sciences, Ilam, Iran. pakzad_i2006@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19293473

Citation

Pakzad, Iraj, et al. "Immunogencity of HSA-L7/L12 (Brucella Abortus Ribosomal Protein) in an Animal Model." Iranian Journal of Immunology : IJI, vol. 6, no. 1, 2009, pp. 12-21.
Pakzad I, Rezaee A, Rasaee MJ, et al. Immunogencity of HSA-L7/L12 (Brucella abortus ribosomal protein) in an animal model. Iran J Immunol. 2009;6(1):12-21.
Pakzad, I., Rezaee, A., Rasaee, M. J., Tabbaraee, B., & Delpisheh, A. (2009). Immunogencity of HSA-L7/L12 (Brucella abortus ribosomal protein) in an animal model. Iranian Journal of Immunology : IJI, 6(1), pp. 12-21. doi:IJIv6i1A2.
Pakzad I, et al. Immunogencity of HSA-L7/L12 (Brucella Abortus Ribosomal Protein) in an Animal Model. Iran J Immunol. 2009;6(1):12-21. PubMed PMID: 19293473.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunogencity of HSA-L7/L12 (Brucella abortus ribosomal protein) in an animal model. AU - Pakzad,Iraj, AU - Rezaee,Abbas, AU - Rasaee,Mohammad Javad, AU - Tabbaraee,Bahman, AU - Delpisheh,Ali, PY - 2009/3/19/entrez PY - 2009/3/19/pubmed PY - 2009/7/17/medline SP - 12 EP - 21 JF - Iranian journal of immunology : IJI JO - Iran J Immunol VL - 6 IS - 1 N2 - BACKGROUND: The immunogenic Brucella abortus ribosomal protein L7/L12 is a promising candidate antigen for the development of subunit vaccines against brucellosis. OBJECTIVE: This study was aimed to evaluate the protection of recombinant Human Serum Albumin (HAS)-L7/L12 fusion protein in Balb/c mice. METHODS: The amplified L7/L12 gene was cloned in pYHSA5 vector, pYHSA5-L7/L12 construct was transformed in Saccharomyces cerevisiae and the expressed protein from supernatant was purified by affinity chromatography. Balb/c mice were immunized in five groups by tHSA-L7/L12 fusion protein (group 1), Brucella abortus S19 (group 2), HSA (group 3), recombinant L7/L12 (group 4), PBS (group 5). ELISA to detect antibody production, LTT test to assess antigen specific lymphocyte response were conducted prior to virulent B. abortus strain 544 challenge two weeks after the last injection. Bacterial counts from spleens of immunized mice were done four weeks after challenge. RESULTS: In ELISA tests, the specific antibodies exhibited a dominance of immunoglobulin IgG1 over IgG2a. In addition, the tHSA-L7/L12 fusion protein and L7/L12 elicited a strong T-cell proliferative response upon restimulation in vitro with recombinant tHSA-L7/L12 and L7/L12, suggesting the induction of a cellular immunity response in vivo. However, there was no significant difference in proliferative response of L7/L12 and tHSA-L7/L12 fusion protein (p>0.05). The L7/L12 and tHSA-L7/L12 fusion protein vaccines could also induce significant protection against challenge with the virulent strain B. abortus 544 in Balb/c mice (p< or =0.05). CONCLUSION: The tHSA-L7/L12 fusion protein, similar to L7/L12 has the ability to induce antigen specific lymphocyte proliferation, stimulate humoral immunity and engender protection. SN - 1735-1383 UR - https://www.unboundmedicine.com/medline/citation/19293473/Immunogencity_of_HSA_L7/L12__Brucella_abortus_ribosomal_protein__in_an_animal_model_ L2 - http://iji.sums.ac.ir/?sid=Entrez:PubMed&amp;id=pmid:19293473&amp;key=2009.6.1.12 DB - PRIME DP - Unbound Medicine ER -