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Sodium benzoate exposure downregulates the expression of tyrosine hydroxylase and dopamine transporter in dopaminergic neurons in developing zebrafish.

Abstract

BACKGROUND

Recent data have demonstrated that treatment with sodium benzoate (SB) leads to significant developmental defects in motor neuron axons and neuromuscular junctions in zebrafish larvae, thereby implying that SB can be neurotoxic. This study examined whether SB affects the development of dopaminergic neurons in the zebrafish brain.

METHODS

Zebrafish embryos were exposed to different concentrations of SB for various durations, during which the survival rates were recorded, the expression of tyrosine hydroxylase (TH) and dopamine transporter (DAT) in the neurons in the ventral diencephalon were detected by in situ hybridization and immunofluorescence, and the locomotor activity of larval zebrafish was measured.

RESULTS

The survival rates were significantly decreased with the increase of duration and dose of SB-treatment. Compared to untreated clutch mates (untreated controls), treatment with SB significantly downregulated expression of TH and DAT in neurons in the ventral diencephalon of 3-day post-fertilization (dpf) zebrafish embryos in a dose-dependent manner. Furthermore, there was a marked decrease in locomotor activity in zebrafish larvae at 6dpf in response to SB treatment.

CONCLUSIONS

The results suggest that SB exposure can cause significantly decreased survival rates of zebrafish embryos in a time- and dose-dependent manner and downregulated expression of TH and DAT in dopaminergic neurons in the zebrafish ventral diencephalon, which results in decreased locomotor activity of zebrafish larvae. This study may provide some important information for further elucidating the mechanism underlying SB-induced developmental neurotoxicity.

Authors+Show Affiliations

Department of Histology and Embryology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, Guangdong Province, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19294673

Citation

Chen, Qian, et al. "Sodium Benzoate Exposure Downregulates the Expression of Tyrosine Hydroxylase and Dopamine Transporter in Dopaminergic Neurons in Developing Zebrafish." Birth Defects Research. Part B, Developmental and Reproductive Toxicology, vol. 86, no. 2, 2009, pp. 85-91.
Chen Q, Huang NN, Huang JT, et al. Sodium benzoate exposure downregulates the expression of tyrosine hydroxylase and dopamine transporter in dopaminergic neurons in developing zebrafish. Birth Defects Res B Dev Reprod Toxicol. 2009;86(2):85-91.
Chen, Q., Huang, N. N., Huang, J. T., Chen, S., Fan, J., Li, C., & Xie, F. K. (2009). Sodium benzoate exposure downregulates the expression of tyrosine hydroxylase and dopamine transporter in dopaminergic neurons in developing zebrafish. Birth Defects Research. Part B, Developmental and Reproductive Toxicology, 86(2), pp. 85-91. doi:10.1002/bdrb.20187.
Chen Q, et al. Sodium Benzoate Exposure Downregulates the Expression of Tyrosine Hydroxylase and Dopamine Transporter in Dopaminergic Neurons in Developing Zebrafish. Birth Defects Res B Dev Reprod Toxicol. 2009;86(2):85-91. PubMed PMID: 19294673.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sodium benzoate exposure downregulates the expression of tyrosine hydroxylase and dopamine transporter in dopaminergic neurons in developing zebrafish. AU - Chen,Qian, AU - Huang,Nan-Nan, AU - Huang,Jin-Tao, AU - Chen,Shen, AU - Fan,Jinjin, AU - Li,Chaohong, AU - Xie,Fu-Kang, PY - 2009/3/19/entrez PY - 2009/3/19/pubmed PY - 2009/6/18/medline SP - 85 EP - 91 JF - Birth defects research. Part B, Developmental and reproductive toxicology JO - Birth Defects Res. B Dev. Reprod. Toxicol. VL - 86 IS - 2 N2 - BACKGROUND: Recent data have demonstrated that treatment with sodium benzoate (SB) leads to significant developmental defects in motor neuron axons and neuromuscular junctions in zebrafish larvae, thereby implying that SB can be neurotoxic. This study examined whether SB affects the development of dopaminergic neurons in the zebrafish brain. METHODS: Zebrafish embryos were exposed to different concentrations of SB for various durations, during which the survival rates were recorded, the expression of tyrosine hydroxylase (TH) and dopamine transporter (DAT) in the neurons in the ventral diencephalon were detected by in situ hybridization and immunofluorescence, and the locomotor activity of larval zebrafish was measured. RESULTS: The survival rates were significantly decreased with the increase of duration and dose of SB-treatment. Compared to untreated clutch mates (untreated controls), treatment with SB significantly downregulated expression of TH and DAT in neurons in the ventral diencephalon of 3-day post-fertilization (dpf) zebrafish embryos in a dose-dependent manner. Furthermore, there was a marked decrease in locomotor activity in zebrafish larvae at 6dpf in response to SB treatment. CONCLUSIONS: The results suggest that SB exposure can cause significantly decreased survival rates of zebrafish embryos in a time- and dose-dependent manner and downregulated expression of TH and DAT in dopaminergic neurons in the zebrafish ventral diencephalon, which results in decreased locomotor activity of zebrafish larvae. This study may provide some important information for further elucidating the mechanism underlying SB-induced developmental neurotoxicity. SN - 1542-9741 UR - https://www.unboundmedicine.com/medline/citation/19294673/Sodium_benzoate_exposure_downregulates_the_expression_of_tyrosine_hydroxylase_and_dopamine_transporter_in_dopaminergic_neurons_in_developing_zebrafish_ L2 - https://doi.org/10.1002/bdrb.20187 DB - PRIME DP - Unbound Medicine ER -