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Chemopreventive efficacy of curcumin and piperine during 7,12-dimethylbenz[a]anthracene-induced hamster buccal pouch carcinogenesis.
Singapore Med J. 2009 Feb; 50(2):139-46.SM

Abstract

INTRODUCTION

Oral carcinoma accounts for 40-50 percent of all cancers in India. Tobacco chewing, smoking and alcohol consumption are the major risk factors associated with the high incidence of oral cancer in India. Our aim was to investigate the chemopreventive potential of curcumin and piperine during 7,12-dimethylbenz[a]anthracene (DMBA)-induced hamster buccal pouch carcinogenesis.

METHODS

Oral squamous cell carcinoma was developed in the buccal pouch of Syrian golden hamsters, by painting them with 0.5 percent DMBA in liquid paraffin, three times a week for 14 weeks. The tumour incidence, tumour volume and burden were determined in the buccal pouches. The status of phase II detoxification agents, lipid peroxidation and antioxidants were estimated by specific colorimetric methods.

RESULTS

We observed 100 percent tumour formation in DMBA-alone painted hamsters. Disturbances in the status of lipid peroxidation, antioxidants and phase II detoxification agents were noticed in DMBA-alone painted hamsters. Oral administration of curcumin (80 mg/kg body weight) and piperine (50 mg/kg body weight) to DMBA-painted hamsters on alternate days to DMBA painting for 14 weeks completely prevented the formation of oral carcinoma. Also, curcumin and piperine restored the status of lipid peroxidation, antioxidants and detoxifying agents in DMBA-painted hamsters.

CONCLUSION

The chemopreventive efficacy of curcumin and piperine is probably due to their antilipidperoxidative and antioxidant potential as well as their modulating effect on the carcinogen detoxification process.

Authors+Show Affiliations

Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar 608002, Tamil Nadu, India. sakshiman@rediffmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19296028

Citation

Manoharan, S, et al. "Chemopreventive Efficacy of Curcumin and Piperine During 7,12-dimethylbenz[a]anthracene-induced Hamster Buccal Pouch Carcinogenesis." Singapore Medical Journal, vol. 50, no. 2, 2009, pp. 139-46.
Manoharan S, Balakrishnan S, Menon VP, et al. Chemopreventive efficacy of curcumin and piperine during 7,12-dimethylbenz[a]anthracene-induced hamster buccal pouch carcinogenesis. Singapore Med J. 2009;50(2):139-46.
Manoharan, S., Balakrishnan, S., Menon, V. P., Alias, L. M., & Reena, A. R. (2009). Chemopreventive efficacy of curcumin and piperine during 7,12-dimethylbenz[a]anthracene-induced hamster buccal pouch carcinogenesis. Singapore Medical Journal, 50(2), 139-46.
Manoharan S, et al. Chemopreventive Efficacy of Curcumin and Piperine During 7,12-dimethylbenz[a]anthracene-induced Hamster Buccal Pouch Carcinogenesis. Singapore Med J. 2009;50(2):139-46. PubMed PMID: 19296028.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chemopreventive efficacy of curcumin and piperine during 7,12-dimethylbenz[a]anthracene-induced hamster buccal pouch carcinogenesis. AU - Manoharan,S, AU - Balakrishnan,S, AU - Menon,V P, AU - Alias,L M, AU - Reena,A R, PY - 2009/3/20/entrez PY - 2009/3/20/pubmed PY - 2009/7/16/medline SP - 139 EP - 46 JF - Singapore medical journal JO - Singapore Med J VL - 50 IS - 2 N2 - INTRODUCTION: Oral carcinoma accounts for 40-50 percent of all cancers in India. Tobacco chewing, smoking and alcohol consumption are the major risk factors associated with the high incidence of oral cancer in India. Our aim was to investigate the chemopreventive potential of curcumin and piperine during 7,12-dimethylbenz[a]anthracene (DMBA)-induced hamster buccal pouch carcinogenesis. METHODS: Oral squamous cell carcinoma was developed in the buccal pouch of Syrian golden hamsters, by painting them with 0.5 percent DMBA in liquid paraffin, three times a week for 14 weeks. The tumour incidence, tumour volume and burden were determined in the buccal pouches. The status of phase II detoxification agents, lipid peroxidation and antioxidants were estimated by specific colorimetric methods. RESULTS: We observed 100 percent tumour formation in DMBA-alone painted hamsters. Disturbances in the status of lipid peroxidation, antioxidants and phase II detoxification agents were noticed in DMBA-alone painted hamsters. Oral administration of curcumin (80 mg/kg body weight) and piperine (50 mg/kg body weight) to DMBA-painted hamsters on alternate days to DMBA painting for 14 weeks completely prevented the formation of oral carcinoma. Also, curcumin and piperine restored the status of lipid peroxidation, antioxidants and detoxifying agents in DMBA-painted hamsters. CONCLUSION: The chemopreventive efficacy of curcumin and piperine is probably due to their antilipidperoxidative and antioxidant potential as well as their modulating effect on the carcinogen detoxification process. SN - 0037-5675 UR - https://www.unboundmedicine.com/medline/citation/19296028/Chemopreventive_efficacy_of_curcumin_and_piperine_during_712_dimethylbenz[a]anthracene_induced_hamster_buccal_pouch_carcinogenesis_ L2 - http://smj.sma.org.sg/5002/5002a4.pdf DB - PRIME DP - Unbound Medicine ER -