Tags

Type your tag names separated by a space and hit enter

Characterization of fluoroquinolone-resistant Shigella flexneri in Hangzhou area of China.
J Antimicrob Chemother. 2009 May; 63(5):917-20.JA

Abstract

OBJECTIVES

The aim of this study was to characterize fluoroquinolone-resistant Shigella and determine whether the qnr and aac(6')-Ib-cr genes could contribute to sporadic shigellosis at the clinic in the Hangzhou area of China.

METHODS

A total of 202 strains of Shigella (79 Shigella sonnei and 123 Shigella flexneri) isolated from sporadic cases of shigellosis from 1998 to 2007 were analysed for their antimicrobial susceptibility. The gyrA, gyrB, parC, parE, qnr and aac(6')-Ib-cr genes and the profiles and incompatibility of plasmids were characterized. Chromosomal DNA fingerprinting was determined by XbaI-based digestion and PFGE.

RESULTS

All strains of S. sonnei were susceptible to fluoroquinolones (ciprofloxacin and levofloxacin) while 15 out of 123 strains of S. flexneri were resistant. All of the 15 resistant strains displayed common mutations in the gyrA and parC genes and formed eight distinct groups with unique molecular characteristics. Notably, 10 isolates showed mutations at codon 87 of gyrA, and the other 5 were qnrS-positive. Two strains were positive for the aac(6')-Ib-cr gene. Importantly, this is the first report of qnrS- and aac(6')-Ib-cr-positive Shigella in China, the qnrS-positive S. flexneri serotypes 1a, 2a and 4c and the aac(6')-Ib-cr-positive S. flexneri serotypes 2a and 4c worldwide.

CONCLUSIONS

The common mutations at position 83 of gyrA and position 80 of parC were crucial for resistance to nalidixic acid in S. flexneri. The mutation at position 87 of gyrA or the presence of the qnrS gene is necessary for high-level resistance to fluoroquinolones in Shigella isolates from China.

Authors+Show Affiliations

Hangzhou Center for Disease Control and Prevention, China. pxygym@126.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19297378

Citation

Pu, Xiao-Ying, et al. "Characterization of Fluoroquinolone-resistant Shigella Flexneri in Hangzhou Area of China." The Journal of Antimicrobial Chemotherapy, vol. 63, no. 5, 2009, pp. 917-20.
Pu XY, Pan JC, Wang HQ, et al. Characterization of fluoroquinolone-resistant Shigella flexneri in Hangzhou area of China. J Antimicrob Chemother. 2009;63(5):917-20.
Pu, X. Y., Pan, J. C., Wang, H. Q., Zhang, W., Huang, Z. C., & Gu, Y. M. (2009). Characterization of fluoroquinolone-resistant Shigella flexneri in Hangzhou area of China. The Journal of Antimicrobial Chemotherapy, 63(5), 917-20. https://doi.org/10.1093/jac/dkp087
Pu XY, et al. Characterization of Fluoroquinolone-resistant Shigella Flexneri in Hangzhou Area of China. J Antimicrob Chemother. 2009;63(5):917-20. PubMed PMID: 19297378.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of fluoroquinolone-resistant Shigella flexneri in Hangzhou area of China. AU - Pu,Xiao-Ying, AU - Pan,Jing-Cao, AU - Wang,Hao-Qiu, AU - Zhang,Wei, AU - Huang,Zhi-Cheng, AU - Gu,Yang-Ming, Y1 - 2009/03/18/ PY - 2009/3/20/entrez PY - 2009/3/20/pubmed PY - 2009/7/8/medline SP - 917 EP - 20 JF - The Journal of antimicrobial chemotherapy JO - J Antimicrob Chemother VL - 63 IS - 5 N2 - OBJECTIVES: The aim of this study was to characterize fluoroquinolone-resistant Shigella and determine whether the qnr and aac(6')-Ib-cr genes could contribute to sporadic shigellosis at the clinic in the Hangzhou area of China. METHODS: A total of 202 strains of Shigella (79 Shigella sonnei and 123 Shigella flexneri) isolated from sporadic cases of shigellosis from 1998 to 2007 were analysed for their antimicrobial susceptibility. The gyrA, gyrB, parC, parE, qnr and aac(6')-Ib-cr genes and the profiles and incompatibility of plasmids were characterized. Chromosomal DNA fingerprinting was determined by XbaI-based digestion and PFGE. RESULTS: All strains of S. sonnei were susceptible to fluoroquinolones (ciprofloxacin and levofloxacin) while 15 out of 123 strains of S. flexneri were resistant. All of the 15 resistant strains displayed common mutations in the gyrA and parC genes and formed eight distinct groups with unique molecular characteristics. Notably, 10 isolates showed mutations at codon 87 of gyrA, and the other 5 were qnrS-positive. Two strains were positive for the aac(6')-Ib-cr gene. Importantly, this is the first report of qnrS- and aac(6')-Ib-cr-positive Shigella in China, the qnrS-positive S. flexneri serotypes 1a, 2a and 4c and the aac(6')-Ib-cr-positive S. flexneri serotypes 2a and 4c worldwide. CONCLUSIONS: The common mutations at position 83 of gyrA and position 80 of parC were crucial for resistance to nalidixic acid in S. flexneri. The mutation at position 87 of gyrA or the presence of the qnrS gene is necessary for high-level resistance to fluoroquinolones in Shigella isolates from China. SN - 1460-2091 UR - https://www.unboundmedicine.com/medline/citation/19297378/Characterization_of_fluoroquinolone_resistant_Shigella_flexneri_in_Hangzhou_area_of_China_ L2 - https://academic.oup.com/jac/article-lookup/doi/10.1093/jac/dkp087 DB - PRIME DP - Unbound Medicine ER -