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RAPD differentiation of Malassezia spp. from cattle, dogs and humans.
Mycoses. 2010 Jan; 53(1):48-56.M

Abstract

The molecular characterization of Malassezia spp. isolates from animals and humans has not been thoroughly studied. We have analysed the DNA profile by random amplified polymorphic DNA (RAPD)-PCR to compare the genetic diversity between isolates from the external ears of cattle, dogs and humans. The analysis of electrophoretic profiles on 8% polyacrylamide gel and their phenograms showed genetic heterogeneity between RAPD profiles of Malassezia furfur and Malassezia slooffiae isolates from humans and cattle and between Malassezia pachydermatis isolates from dogs and cattle. Intra-species variations in DNA pattern of Malassezia isolates and the presence of specific genetic types in cattle, dogs or humans were observed. A review of genetic heterogeneity of these yeast in veterinary and human medicine studies is given considering a possible transmission animal to human or human to animal. Additional studies must clarify the differences between the RAPD band patterns observed in this and other studies, which would facilitate monitoring of Malassezia spp. carriage in domestic animals and in humans.

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Authors+Show Affiliations

Duarte ER
Instituto de Ciências Agrárias, Universidade Federal de Minas Gerais, Montes Claros, MG, Brazil. duartevet@hotmail.com
Hamdan JS
No affiliation info available

MeSH

AnimalsCattleCluster AnalysisDNA FingerprintingDNA, FungalDermatomycosesDogsElectrophoresis, Polyacrylamide GelGenetic VariationGenotypeHumansMalasseziaMycological Typing TechniquesRandom Amplified Polymorphic DNA Technique

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

19298360

Citation

Duarte, Eduardo R., and Júnia S. Hamdan. "RAPD Differentiation of Malassezia Spp. From Cattle, Dogs and Humans." Mycoses, vol. 53, no. 1, 2010, pp. 48-56.
Duarte ER, Hamdan JS. RAPD differentiation of Malassezia spp. from cattle, dogs and humans. Mycoses. 2010;53(1):48-56.
Duarte, E. R., & Hamdan, J. S. (2010). RAPD differentiation of Malassezia spp. from cattle, dogs and humans. Mycoses, 53(1), 48-56. https://doi.org/10.1111/j.1439-0507.2008.01658.x
Duarte ER, Hamdan JS. RAPD Differentiation of Malassezia Spp. From Cattle, Dogs and Humans. Mycoses. 2010;53(1):48-56. PubMed PMID: 19298360.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - RAPD differentiation of Malassezia spp. from cattle, dogs and humans. AU - Duarte,Eduardo R, AU - Hamdan,Júnia S, Y1 - 2009/03/03/ PY - 2009/3/21/entrez PY - 2009/3/21/pubmed PY - 2010/2/18/medline SP - 48 EP - 56 JF - Mycoses JO - Mycoses VL - 53 IS - 1 N2 - The molecular characterization of Malassezia spp. isolates from animals and humans has not been thoroughly studied. We have analysed the DNA profile by random amplified polymorphic DNA (RAPD)-PCR to compare the genetic diversity between isolates from the external ears of cattle, dogs and humans. The analysis of electrophoretic profiles on 8% polyacrylamide gel and their phenograms showed genetic heterogeneity between RAPD profiles of Malassezia furfur and Malassezia slooffiae isolates from humans and cattle and between Malassezia pachydermatis isolates from dogs and cattle. Intra-species variations in DNA pattern of Malassezia isolates and the presence of specific genetic types in cattle, dogs or humans were observed. A review of genetic heterogeneity of these yeast in veterinary and human medicine studies is given considering a possible transmission animal to human or human to animal. Additional studies must clarify the differences between the RAPD band patterns observed in this and other studies, which would facilitate monitoring of Malassezia spp. carriage in domestic animals and in humans. SN - 1439-0507 UR - https://www.unboundmedicine.com/medline/citation/19298360/RAPD_differentiation_of_Malassezia_spp__from_cattle_dogs_and_humans_ L2 - https://doi.org/10.1111/j.1439-0507.2008.01658.x DB - PRIME DP - Unbound Medicine ER -