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HPLC determination of eight polyphenols in the leaves of Crataegus pinnatifida Bge. var. major.
J Chromatogr Sci. 2009 Mar; 47(3):201-5.JC

Abstract

A simple high-performance liquid chromatographic (HPLC) assay using the internal standard method is developed for the simultaneous determination of eight polyphenols. The analyzed compounds isolated from the leaves of Crataegus pinnatifida Bge. var. major include chlorogenic acid, vitexin-4"-O-glucoside, vitexin-2"-O-rhamnoside, vitexin, rutin, hyperoside, isoquercitrin, and quercetin. HPLC analysis is performed on a Diamonsil C18 analytical column (150 x 4.6 mm, i.d., 5-microm) using solvent (A) acetonitrile-tetrahydrofuran (95:5, v/v) and (B) 1% aqueous phosphoric acid as the mobile phase with UV absorption at 270 nm. The calibration curves of the eight polyphenols are linear (r(2) > 0.9992) over the concentration range of 0.0894-120.0 microg/mL. The mean recoveries are 95.4% to 98.1%. The results indicate that the HPLC method developed can easily be applied to the determination of eight polyphenols in the leaves of Crataegus pinnatifida Bge. var. major.

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Authors+Show Affiliations

Ying X
School of Pharmacy, Liaoning University of TCM, 79 Chongshan East Road, Shenyang, Liaoning Province, PR China.
Wang R
No affiliation info available
Xu J
No affiliation info available
Zhang W
No affiliation info available
Li H
No affiliation info available
Zhang C
No affiliation info available
Li F
No affiliation info available

MeSH

Chromatography, High Pressure LiquidCrataegusDrug StabilityFlavonoidsIndicators and ReagentsPhenolsPlant LeavesPolyphenolsQuality ControlReproducibility of Results

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19298706

Citation

Ying, Xixiang, et al. "HPLC Determination of Eight Polyphenols in the Leaves of Crataegus Pinnatifida Bge. Var. Major." Journal of Chromatographic Science, vol. 47, no. 3, 2009, pp. 201-5.
Ying X, Wang R, Xu J, et al. HPLC determination of eight polyphenols in the leaves of Crataegus pinnatifida Bge. var. major. J Chromatogr Sci. 2009;47(3):201-5.
Ying, X., Wang, R., Xu, J., Zhang, W., Li, H., Zhang, C., & Li, F. (2009). HPLC determination of eight polyphenols in the leaves of Crataegus pinnatifida Bge. var. major. Journal of Chromatographic Science, 47(3), 201-5.
Ying X, et al. HPLC Determination of Eight Polyphenols in the Leaves of Crataegus Pinnatifida Bge. Var. Major. J Chromatogr Sci. 2009;47(3):201-5. PubMed PMID: 19298706.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - HPLC determination of eight polyphenols in the leaves of Crataegus pinnatifida Bge. var. major. AU - Ying,Xixiang, AU - Wang,Rongxiang, AU - Xu,Jing, AU - Zhang,Wenjie, AU - Li,Haibo, AU - Zhang,Chaoshen, AU - Li,Famei, PY - 2009/3/21/entrez PY - 2009/3/21/pubmed PY - 2009/5/9/medline SP - 201 EP - 5 JF - Journal of chromatographic science JO - J Chromatogr Sci VL - 47 IS - 3 N2 - A simple high-performance liquid chromatographic (HPLC) assay using the internal standard method is developed for the simultaneous determination of eight polyphenols. The analyzed compounds isolated from the leaves of Crataegus pinnatifida Bge. var. major include chlorogenic acid, vitexin-4"-O-glucoside, vitexin-2"-O-rhamnoside, vitexin, rutin, hyperoside, isoquercitrin, and quercetin. HPLC analysis is performed on a Diamonsil C18 analytical column (150 x 4.6 mm, i.d., 5-microm) using solvent (A) acetonitrile-tetrahydrofuran (95:5, v/v) and (B) 1% aqueous phosphoric acid as the mobile phase with UV absorption at 270 nm. The calibration curves of the eight polyphenols are linear (r(2) > 0.9992) over the concentration range of 0.0894-120.0 microg/mL. The mean recoveries are 95.4% to 98.1%. The results indicate that the HPLC method developed can easily be applied to the determination of eight polyphenols in the leaves of Crataegus pinnatifida Bge. var. major. SN - 0021-9665 UR - https://www.unboundmedicine.com/medline/citation/19298706/HPLC_determination_of_eight_polyphenols_in_the_leaves_of_Crataegus_pinnatifida_Bge__var__major_ L2 - https://academic.oup.com/chromsci/article-lookup/doi/10.1093/chromsci/47.3.201 DB - PRIME DP - Unbound Medicine ER -