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Select nutrients in the ovine uterine lumen. VI. Expression of FK506-binding protein 12-rapamycin complex-associated protein 1 (FRAP1) and regulators and effectors of mTORC1 and mTORC2 complexes in ovine uteri and conceptuses.
Biol Reprod. 2009 Jul; 81(1):87-100.BR

Abstract

FRAP1 (FK506-binding protein 12-rapamycin complex-associated protein 1), a component of the nutrient-sensing cell signaling pathway, is critical for cell growth and metabolism. The present study determined expression of FRAP1 and associated members of the mTORC1 and mTORC2 cell signaling pathways in uteri of cyclic and pregnant ewes and conceptuses, as well as effects of pregnancy, progesterone (P4), and interferon tau (IFNT) on their expression. The mRNAs for FRAP1, LST8, MAPKAP1, RAPTOR, RICTOR, TSC1, TSC2, RHEB, and EIF4EBP1 were localized to luminal, superficial glandular, and glandular epithelia and stromal cells of uteri from cyclic and pregnant ewes, as well as trophectoderm and endoderm of conceptuses between Days 13 and 18 of pregnancy. The abundance of FRAP1, RAPTOR, RICTOR, TSC1, and TSC2 mRNAs in endometria was unaffected by pregnancy status or by day of the estrous cycle or pregnancy; however, levels of LST8, MAPKAP1, RHEB, and EIF4EBP1 mRNA increased in endometria during early pregnancy. In ovariectomized ewes, P4 and IFNT stimulated expression of RHEB and EIF4EBP1 in uterine endometria. Total endometrial FRAP1 protein and phosphorylated FRAP1 protein levels were affected by pregnancy status and by day after onset of estrus, and phosphorylated FRAP1 protein was detected in nuclei of uterine epithelia and conceptuses. In endometria of pregnant ewes, increases in abundance of mRNAs for RICTOR, RHEB, and EIF4EBP1, as well as RHEB protein, correlated with rapid conceptus growth and development during the peri-implantation period. These results suggest that the FRAP1 cell signaling pathway mediates interactions between the maternal uterus and peri-implantation conceptuses and that P4 and IFNT affect this pathway by regulating expression of RHEB and EIF4EBP1.

Authors+Show Affiliations

Center for Animal Biotechnology and Genomics, Texas A&M University, College Station, Texas 77843-2471, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Randomized Controlled Trial
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

19299312

Citation

Gao, Haijun, et al. "Select Nutrients in the Ovine Uterine Lumen. VI. Expression of FK506-binding Protein 12-rapamycin Complex-associated Protein 1 (FRAP1) and Regulators and Effectors of mTORC1 and mTORC2 Complexes in Ovine Uteri and Conceptuses." Biology of Reproduction, vol. 81, no. 1, 2009, pp. 87-100.
Gao H, Wu G, Spencer TE, et al. Select nutrients in the ovine uterine lumen. VI. Expression of FK506-binding protein 12-rapamycin complex-associated protein 1 (FRAP1) and regulators and effectors of mTORC1 and mTORC2 complexes in ovine uteri and conceptuses. Biol Reprod. 2009;81(1):87-100.
Gao, H., Wu, G., Spencer, T. E., Johnson, G. A., & Bazer, F. W. (2009). Select nutrients in the ovine uterine lumen. VI. Expression of FK506-binding protein 12-rapamycin complex-associated protein 1 (FRAP1) and regulators and effectors of mTORC1 and mTORC2 complexes in ovine uteri and conceptuses. Biology of Reproduction, 81(1), 87-100. https://doi.org/10.1095/biolreprod.109.076257
Gao H, et al. Select Nutrients in the Ovine Uterine Lumen. VI. Expression of FK506-binding Protein 12-rapamycin Complex-associated Protein 1 (FRAP1) and Regulators and Effectors of mTORC1 and mTORC2 Complexes in Ovine Uteri and Conceptuses. Biol Reprod. 2009;81(1):87-100. PubMed PMID: 19299312.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Select nutrients in the ovine uterine lumen. VI. Expression of FK506-binding protein 12-rapamycin complex-associated protein 1 (FRAP1) and regulators and effectors of mTORC1 and mTORC2 complexes in ovine uteri and conceptuses. AU - Gao,Haijun, AU - Wu,Guoyao, AU - Spencer,Thomas E, AU - Johnson,Greg A, AU - Bazer,Fuller W, Y1 - 2009/03/18/ PY - 2009/3/21/entrez PY - 2009/3/21/pubmed PY - 2009/9/23/medline SP - 87 EP - 100 JF - Biology of reproduction JO - Biol. Reprod. VL - 81 IS - 1 N2 - FRAP1 (FK506-binding protein 12-rapamycin complex-associated protein 1), a component of the nutrient-sensing cell signaling pathway, is critical for cell growth and metabolism. The present study determined expression of FRAP1 and associated members of the mTORC1 and mTORC2 cell signaling pathways in uteri of cyclic and pregnant ewes and conceptuses, as well as effects of pregnancy, progesterone (P4), and interferon tau (IFNT) on their expression. The mRNAs for FRAP1, LST8, MAPKAP1, RAPTOR, RICTOR, TSC1, TSC2, RHEB, and EIF4EBP1 were localized to luminal, superficial glandular, and glandular epithelia and stromal cells of uteri from cyclic and pregnant ewes, as well as trophectoderm and endoderm of conceptuses between Days 13 and 18 of pregnancy. The abundance of FRAP1, RAPTOR, RICTOR, TSC1, and TSC2 mRNAs in endometria was unaffected by pregnancy status or by day of the estrous cycle or pregnancy; however, levels of LST8, MAPKAP1, RHEB, and EIF4EBP1 mRNA increased in endometria during early pregnancy. In ovariectomized ewes, P4 and IFNT stimulated expression of RHEB and EIF4EBP1 in uterine endometria. Total endometrial FRAP1 protein and phosphorylated FRAP1 protein levels were affected by pregnancy status and by day after onset of estrus, and phosphorylated FRAP1 protein was detected in nuclei of uterine epithelia and conceptuses. In endometria of pregnant ewes, increases in abundance of mRNAs for RICTOR, RHEB, and EIF4EBP1, as well as RHEB protein, correlated with rapid conceptus growth and development during the peri-implantation period. These results suggest that the FRAP1 cell signaling pathway mediates interactions between the maternal uterus and peri-implantation conceptuses and that P4 and IFNT affect this pathway by regulating expression of RHEB and EIF4EBP1. SN - 0006-3363 UR - https://www.unboundmedicine.com/medline/citation/19299312/Select_nutrients_in_the_ovine_uterine_lumen__VI__Expression_of_FK506_binding_protein_12_rapamycin_complex_associated_protein_1__FRAP1__and_regulators_and_effectors_of_mTORC1_and_mTORC2_complexes_in_ovine_uteri_and_conceptuses_ L2 - https://academic.oup.com/biolreprod/article-lookup/doi/10.1095/biolreprod.109.076257 DB - PRIME DP - Unbound Medicine ER -