TY - JOUR T1 - Oxidative stress sensitivity in Debaryomyces hansenii. AU - Navarrete,Clara, AU - Siles,Alicia, AU - Martínez,José L, AU - Calero,Fernando, AU - Ramos,José, Y1 - 2009/03/19/ PY - 2009/3/24/entrez PY - 2009/3/24/pubmed PY - 2009/7/16/medline SP - 582 EP - 90 JF - FEMS yeast research JO - FEMS Yeast Res VL - 9 IS - 4 N2 - Debaryomyces hansenii is an osmotolerant and halotolerant yeast of increasing interest for fundamental and applied research. In this work, we have performed a first study on the effect of oxidative stress on the performance of this yeast. We have used Saccharomyces cerevisiae as a well-known reference yeast. We show that D. hansenii is much more susceptible than S. cerevisiae to cadmium chloride, hydrogen peroxide or 1,4-dithiothreitol. These substances induced the formation of reactive oxygen species (ROS) in both yeasts, the amounts measured being significantly higher in the case of D. hansenii. We also show that NaCl exerted a protective effect against oxidative stress in Debaryomyces, but that this was not the case in Saccharomyces because sodium protected that yeast only when toxicity was induced with cadmium. On the basis of the present results, we raised the hypothesis that the sensitivity to oxidative stress in D. hansenii is related to the high amounts of ROS formed in that yeast and that observations such as low glutathione amounts, low basal superoxide dismutase and peroxidase activities, decrease in ATP levels produced in the presence of ROS inducers and high cadmium accumulation are determinants directly or indirectly involved in the sensitivity process. SN - 1567-1364 UR - https://www.unboundmedicine.com/medline/citation/19302096/Oxidative_stress_sensitivity_in_Debaryomyces_hansenii_ L2 - https://academic.oup.com/femsyr/article-lookup/doi/10.1111/j.1567-1364.2009.00500.x DB - PRIME DP - Unbound Medicine ER -