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Rapid extraction combined with LC-tandem mass spectrometry (CREM-LC/MS/MS) for the determination of ciguatoxins in ciguateric fish flesh.
Toxicon. 2009 Jul; 54(1):62-6.T

Abstract

Ciguatera is a significant food borne disease caused by potent polyether toxins known as ciguatoxins, which accumulate in the flesh of ciguateric fish at risk levels above 0.1 ppb. The management of ciguatera has been hindered by the lack of analytical methods to detect and quantify clinically relevant levels of ciguatoxin in easily prepared crude extracts of fish. Here we report a ciguatoxin rapid extraction method (CREM) that allows the rapid preparation of fish flesh extracts for the detection and quantification of ciguatoxin by gradient reversed-phase liquid chromatography-tandem mass spectrometry (LC/MS/MS). CREM-LC/MS/MS delivers a linear response to P-CTX-1 spiked into fish prior to extraction. A similar response was obtained for P-CTX-1 spiked after extraction, indicating >95% extraction efficiency was achieved overall and 85% at the limit of quantification (0.1 ppb). Using this approach, levels >or=0.1 ppb P-CTX-1 could be detected and quantified from an extract of 2g fish flesh, making it suitable as a confirmatory assay for suspect ciguateric carnivorous fish in the Pacific Ocean. The approach is designed to simplify the extraction and analysis of multiple samples per day.

Authors+Show Affiliations

Institute for Molecular Bioscience, The University of Queensland, Qld 4072, Australia. r.lewis@imb.uq.edu.auNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19303895

Citation

Lewis, Richard J., et al. "Rapid Extraction Combined With LC-tandem Mass Spectrometry (CREM-LC/MS/MS) for the Determination of Ciguatoxins in Ciguateric Fish Flesh." Toxicon : Official Journal of the International Society On Toxinology, vol. 54, no. 1, 2009, pp. 62-6.
Lewis RJ, Yang A, Jones A. Rapid extraction combined with LC-tandem mass spectrometry (CREM-LC/MS/MS) for the determination of ciguatoxins in ciguateric fish flesh. Toxicon. 2009;54(1):62-6.
Lewis, R. J., Yang, A., & Jones, A. (2009). Rapid extraction combined with LC-tandem mass spectrometry (CREM-LC/MS/MS) for the determination of ciguatoxins in ciguateric fish flesh. Toxicon : Official Journal of the International Society On Toxinology, 54(1), 62-6. https://doi.org/10.1016/j.toxicon.2009.03.013
Lewis RJ, Yang A, Jones A. Rapid Extraction Combined With LC-tandem Mass Spectrometry (CREM-LC/MS/MS) for the Determination of Ciguatoxins in Ciguateric Fish Flesh. Toxicon. 2009;54(1):62-6. PubMed PMID: 19303895.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid extraction combined with LC-tandem mass spectrometry (CREM-LC/MS/MS) for the determination of ciguatoxins in ciguateric fish flesh. AU - Lewis,Richard J, AU - Yang,Aijun, AU - Jones,Alun, Y1 - 2009/03/19/ PY - 2008/10/17/received PY - 2009/03/11/revised PY - 2009/03/12/accepted PY - 2009/3/24/entrez PY - 2009/3/24/pubmed PY - 2009/9/16/medline SP - 62 EP - 6 JF - Toxicon : official journal of the International Society on Toxinology JO - Toxicon VL - 54 IS - 1 N2 - Ciguatera is a significant food borne disease caused by potent polyether toxins known as ciguatoxins, which accumulate in the flesh of ciguateric fish at risk levels above 0.1 ppb. The management of ciguatera has been hindered by the lack of analytical methods to detect and quantify clinically relevant levels of ciguatoxin in easily prepared crude extracts of fish. Here we report a ciguatoxin rapid extraction method (CREM) that allows the rapid preparation of fish flesh extracts for the detection and quantification of ciguatoxin by gradient reversed-phase liquid chromatography-tandem mass spectrometry (LC/MS/MS). CREM-LC/MS/MS delivers a linear response to P-CTX-1 spiked into fish prior to extraction. A similar response was obtained for P-CTX-1 spiked after extraction, indicating >95% extraction efficiency was achieved overall and 85% at the limit of quantification (0.1 ppb). Using this approach, levels >or=0.1 ppb P-CTX-1 could be detected and quantified from an extract of 2g fish flesh, making it suitable as a confirmatory assay for suspect ciguateric carnivorous fish in the Pacific Ocean. The approach is designed to simplify the extraction and analysis of multiple samples per day. SN - 1879-3150 UR - https://www.unboundmedicine.com/medline/citation/19303895/Rapid_extraction_combined_with_LC_tandem_mass_spectrometry__CREM_LC/MS/MS__for_the_determination_of_ciguatoxins_in_ciguateric_fish_flesh_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-0101(09)00167-6 DB - PRIME DP - Unbound Medicine ER -