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Role of rat sensory neuron-specific receptor (rSNSR1) in inflammatory pain: contribution of TRPV1 to SNSR signaling in the pain pathway.
Pain. 2009 May; 143(1-2):130-7.PAIN

Abstract

Sensory neuron-specific receptors (SNSRs) belong to a large family of GPCRs, known as Mrgs (Mas-related genes), many of which are preferentially expressed in primary afferent nociceptors. Selective SNSR agonists produce pain-like behaviors in rats, showing that SNSR activation is sufficient to produce pain. However, it is unknown whether SNSR activation is necessary for pain either in the normal condition or in pathological pain states. Here we used small interfering RNA (siRNA) to acutely knockdown rat SNSR1 and test the hypothesis that this receptor mediates pain responses. Administration of siRNA to the lumbar spinal cord in rats dose-dependently knocked down rSNSR1 mRNA and protein and abolished heat hyperalgesia evoked by intradermal administration of specific rSNSR1 agonists. In rats with levels of rSNSR1 knockdown sufficient to block responses to the SNSR1 agonists, there was no effect on normal pain responses, but there was a significant reduction of heat hyperalgesia in an inflammatory pain model (Complete Freund's Adjuvant), supporting a role for rSNSR1 in inflammatory pain. Further in vivo studies revealed that SNSR1 knockdown had no effect on responses to intradermal capsaicin, a selective TRPV1 agonist. In contrast, a selective TRPV1 antagonist abolished heat hyperalgesia produced by an SNSR agonist, suggesting that TRPV1 receptors mediate rSNSR1-evoked responses. We also found that rSNSR1-like immunoreactivity, like TRPV1, is localized in the superficial dorsal horn of the spinal cord. We propose that rSNSR1 represents a new member of the receptors expressed on chemosensitive nociceptors responsible for detecting the "inflammatory soup" of mediators generated by tissue damage.

Authors+Show Affiliations

AstraZeneca R&D Montréal, 7171 Frédérick-Banting, Ville Saint-Laurent, Que., Canada H4S 1Z9.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19307060

Citation

Ndong, Christian, et al. "Role of Rat Sensory Neuron-specific Receptor (rSNSR1) in Inflammatory Pain: Contribution of TRPV1 to SNSR Signaling in the Pain Pathway." Pain, vol. 143, no. 1-2, 2009, pp. 130-7.
Ndong C, Pradhan A, Puma C, et al. Role of rat sensory neuron-specific receptor (rSNSR1) in inflammatory pain: contribution of TRPV1 to SNSR signaling in the pain pathway. Pain. 2009;143(1-2):130-7.
Ndong, C., Pradhan, A., Puma, C., Morello, J. P., Hoffert, C., Groblewski, T., O'Donnell, D., & Laird, J. M. (2009). Role of rat sensory neuron-specific receptor (rSNSR1) in inflammatory pain: contribution of TRPV1 to SNSR signaling in the pain pathway. Pain, 143(1-2), 130-7. https://doi.org/10.1016/j.pain.2009.02.010
Ndong C, et al. Role of Rat Sensory Neuron-specific Receptor (rSNSR1) in Inflammatory Pain: Contribution of TRPV1 to SNSR Signaling in the Pain Pathway. Pain. 2009;143(1-2):130-7. PubMed PMID: 19307060.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of rat sensory neuron-specific receptor (rSNSR1) in inflammatory pain: contribution of TRPV1 to SNSR signaling in the pain pathway. AU - Ndong,Christian, AU - Pradhan,Amynah, AU - Puma,Carole, AU - Morello,Jean-Pierre, AU - Hoffert,Cyrla, AU - Groblewski,Thierry, AU - O'Donnell,Dajan, AU - Laird,Jennifer M A, PY - 2008/08/08/received PY - 2009/01/07/revised PY - 2009/02/17/accepted PY - 2009/3/25/entrez PY - 2009/3/25/pubmed PY - 2009/7/21/medline SP - 130 EP - 7 JF - Pain JO - Pain VL - 143 IS - 1-2 N2 - Sensory neuron-specific receptors (SNSRs) belong to a large family of GPCRs, known as Mrgs (Mas-related genes), many of which are preferentially expressed in primary afferent nociceptors. Selective SNSR agonists produce pain-like behaviors in rats, showing that SNSR activation is sufficient to produce pain. However, it is unknown whether SNSR activation is necessary for pain either in the normal condition or in pathological pain states. Here we used small interfering RNA (siRNA) to acutely knockdown rat SNSR1 and test the hypothesis that this receptor mediates pain responses. Administration of siRNA to the lumbar spinal cord in rats dose-dependently knocked down rSNSR1 mRNA and protein and abolished heat hyperalgesia evoked by intradermal administration of specific rSNSR1 agonists. In rats with levels of rSNSR1 knockdown sufficient to block responses to the SNSR1 agonists, there was no effect on normal pain responses, but there was a significant reduction of heat hyperalgesia in an inflammatory pain model (Complete Freund's Adjuvant), supporting a role for rSNSR1 in inflammatory pain. Further in vivo studies revealed that SNSR1 knockdown had no effect on responses to intradermal capsaicin, a selective TRPV1 agonist. In contrast, a selective TRPV1 antagonist abolished heat hyperalgesia produced by an SNSR agonist, suggesting that TRPV1 receptors mediate rSNSR1-evoked responses. We also found that rSNSR1-like immunoreactivity, like TRPV1, is localized in the superficial dorsal horn of the spinal cord. We propose that rSNSR1 represents a new member of the receptors expressed on chemosensitive nociceptors responsible for detecting the "inflammatory soup" of mediators generated by tissue damage. SN - 1872-6623 UR - https://www.unboundmedicine.com/medline/citation/19307060/Role_of_rat_sensory_neuron_specific_receptor__rSNSR1__in_inflammatory_pain:_contribution_of_TRPV1_to_SNSR_signaling_in_the_pain_pathway_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3959(09)00128-6 DB - PRIME DP - Unbound Medicine ER -