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Increased chitotriosidase activity in serum of leprosy patients: association with bacillary leprosy.
Clin Immunol. 2009 Jun; 131(3):501-9.CI

Abstract

Human phagocyte-specific chitotriosidase is associated with several diseases involving macrophage activation. Since macrophage activation plays an important role in the control of Mycobacterium leprae infection, we studied the association of chitotriosidase with leprosy both in serum and in situ in lesional skin biopsies from patients. Serum samples from 78 Indonesian leprosy patients (39 non-reactional and 39 reactional leprosy patients) and 36 healthy controls (HC) from the same endemic region were investigated. The patients were classified as multibacillary (MB, n=69) or paucibacillary (PB, n=9) based on the bacterial index in slit-skin smears. Thirty-six of the reactional patients had erythema nodosum leprosum (ENL), while only 3 had reversal reaction (RR). Follow-up serum samples after corticosteroid treatment were also obtained from 17 patients with ENL and one with RR. Multibacillary (MB) patients showed increased chitotriosidase activity in serum as compared to paucibacillary (PB) patients and healthy controls. Although no significant difference was observed between reactional and the corresponding non-reactional groups, ENL showed significantly higher chitotriosidase activity as compared to HC. Furthermore, corticosteroid treatment resulted in significant decline of enzyme activity in ENL sera. Chitotriosidase activity correlated with levels of neopterin, another macrophage activation marker, but not with IL-6, IFN-gamma, TNF-alpha and IL-10. Immunohistochemical staining of 6 MB (LL=5, BL=1) lesional skin sections from stored material showed positive staining for chitotriosidase within lipid-laden macrophages suggesting that macrophages are the source of the enzyme detected in serum. Thus, serum chitotriosidase activity is potentially useful in distinguishing MB from PB leprosy and in monitoring response to therapy in ENL.

Authors+Show Affiliations

Department of Pathology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19307157

Citation

Iyer, Anand, et al. "Increased Chitotriosidase Activity in Serum of Leprosy Patients: Association With Bacillary Leprosy." Clinical Immunology (Orlando, Fla.), vol. 131, no. 3, 2009, pp. 501-9.
Iyer A, van Eijk M, Silva E, et al. Increased chitotriosidase activity in serum of leprosy patients: association with bacillary leprosy. Clin Immunol. 2009;131(3):501-9.
Iyer, A., van Eijk, M., Silva, E., Hatta, M., Faber, W., Aerts, J. M., & Das, P. K. (2009). Increased chitotriosidase activity in serum of leprosy patients: association with bacillary leprosy. Clinical Immunology (Orlando, Fla.), 131(3), 501-9. https://doi.org/10.1016/j.clim.2009.02.003
Iyer A, et al. Increased Chitotriosidase Activity in Serum of Leprosy Patients: Association With Bacillary Leprosy. Clin Immunol. 2009;131(3):501-9. PubMed PMID: 19307157.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increased chitotriosidase activity in serum of leprosy patients: association with bacillary leprosy. AU - Iyer,Anand, AU - van Eijk,Marco, AU - Silva,Eliane, AU - Hatta,Mochammad, AU - Faber,William, AU - Aerts,Johannes M F G, AU - Das,Pranab Kumar, PY - 2008/05/01/received PY - 2009/02/10/revised PY - 2009/02/11/accepted PY - 2009/3/25/entrez PY - 2009/3/25/pubmed PY - 2009/6/10/medline SP - 501 EP - 9 JF - Clinical immunology (Orlando, Fla.) JO - Clin Immunol VL - 131 IS - 3 N2 - Human phagocyte-specific chitotriosidase is associated with several diseases involving macrophage activation. Since macrophage activation plays an important role in the control of Mycobacterium leprae infection, we studied the association of chitotriosidase with leprosy both in serum and in situ in lesional skin biopsies from patients. Serum samples from 78 Indonesian leprosy patients (39 non-reactional and 39 reactional leprosy patients) and 36 healthy controls (HC) from the same endemic region were investigated. The patients were classified as multibacillary (MB, n=69) or paucibacillary (PB, n=9) based on the bacterial index in slit-skin smears. Thirty-six of the reactional patients had erythema nodosum leprosum (ENL), while only 3 had reversal reaction (RR). Follow-up serum samples after corticosteroid treatment were also obtained from 17 patients with ENL and one with RR. Multibacillary (MB) patients showed increased chitotriosidase activity in serum as compared to paucibacillary (PB) patients and healthy controls. Although no significant difference was observed between reactional and the corresponding non-reactional groups, ENL showed significantly higher chitotriosidase activity as compared to HC. Furthermore, corticosteroid treatment resulted in significant decline of enzyme activity in ENL sera. Chitotriosidase activity correlated with levels of neopterin, another macrophage activation marker, but not with IL-6, IFN-gamma, TNF-alpha and IL-10. Immunohistochemical staining of 6 MB (LL=5, BL=1) lesional skin sections from stored material showed positive staining for chitotriosidase within lipid-laden macrophages suggesting that macrophages are the source of the enzyme detected in serum. Thus, serum chitotriosidase activity is potentially useful in distinguishing MB from PB leprosy and in monitoring response to therapy in ENL. SN - 1521-7035 UR - https://www.unboundmedicine.com/medline/citation/19307157/Increased_chitotriosidase_activity_in_serum_of_leprosy_patients:_association_with_bacillary_leprosy_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1521-6616(09)00042-4 DB - PRIME DP - Unbound Medicine ER -