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Bacteria, biofilm and honey: a study of the effects of honey on 'planktonic' and biofilm-embedded chronic wound bacteria.

Abstract

Chronically infected wounds are a costly source of suffering. An important factor in the failure of a sore to heal is the presence of multiple species of bacteria, living cooperatively in highly organized biofilms. The biofilm protects the bacteria from antibiotic therapy and the patient's immune response. Honey has been used as a wound treatment for millennia. The components responsible for its antibacterial properties are now being elucidated. The study aimed to determine the effects of different concentrations of 'Medihoney' therapeutic honey and Norwegian Forest Honey 1) on the real-time growth of typical chronic wound bacteria; 2) on biofilm formation; and 3) on the same bacteria already embedded in biofilm. Reference strains of MRSE, MRSA, ESBL Klebsiella pneumoniae and Pseudomonas aeruginosa were incubated with dilution series of the honeys in microtitre plates for 20 h. Growth of the bacteria was assessed by measuring optical density every 10 min. Growth curves, biofilm formation and minimum bactericidal concentrations are presented. Both honeys were bactericidal against all the strains of bacteria. Biofilm was penetrated by biocidal substances in honey. Reintroduction of honey as a conventional wound treatment may help improve individual wound care, prevent invasive infections, eliminate colonization, interrupt outbreaks and thereby preserve current antibiotic stocks.

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  • Authors+Show Affiliations

    ,

    Department of Microbiology, Oslo University Hospital, Ullevål and the Faculty of Medicine, University of Oslo, Oslo, Norway. patricia.merckoll@ulleval.no

    , , ,

    Source

    MeSH

    Anti-Bacterial Agents
    Bandages
    Biofilms
    Honey
    Humans
    Klebsiella pneumoniae
    Methicillin
    Methicillin Resistance
    Methicillin-Resistant Staphylococcus aureus
    Pseudomonas aeruginosa
    Staphylococcus epidermidis
    Wound Healing
    Wounds and Injuries

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    19308800

    Citation

    Merckoll, Patricia, et al. "Bacteria, Biofilm and Honey: a Study of the Effects of Honey On 'planktonic' and Biofilm-embedded Chronic Wound Bacteria." Scandinavian Journal of Infectious Diseases, vol. 41, no. 5, 2009, pp. 341-7.
    Merckoll P, Jonassen TØ, Vad ME, et al. Bacteria, biofilm and honey: a study of the effects of honey on 'planktonic' and biofilm-embedded chronic wound bacteria. Scand J Infect Dis. 2009;41(5):341-7.
    Merckoll, P., Jonassen, T. Ø., Vad, M. E., Jeansson, S. L., & Melby, K. K. (2009). Bacteria, biofilm and honey: a study of the effects of honey on 'planktonic' and biofilm-embedded chronic wound bacteria. Scandinavian Journal of Infectious Diseases, 41(5), pp. 341-7. doi:10.1080/00365540902849383.
    Merckoll P, et al. Bacteria, Biofilm and Honey: a Study of the Effects of Honey On 'planktonic' and Biofilm-embedded Chronic Wound Bacteria. Scand J Infect Dis. 2009;41(5):341-7. PubMed PMID: 19308800.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Bacteria, biofilm and honey: a study of the effects of honey on 'planktonic' and biofilm-embedded chronic wound bacteria. AU - Merckoll,Patricia, AU - Jonassen,Tom Øystein, AU - Vad,Marie Elisabeth, AU - Jeansson,Stig L, AU - Melby,Kjetil K, PY - 2009/3/25/entrez PY - 2009/3/25/pubmed PY - 2009/7/3/medline SP - 341 EP - 7 JF - Scandinavian journal of infectious diseases JO - Scand. J. Infect. Dis. VL - 41 IS - 5 N2 - Chronically infected wounds are a costly source of suffering. An important factor in the failure of a sore to heal is the presence of multiple species of bacteria, living cooperatively in highly organized biofilms. The biofilm protects the bacteria from antibiotic therapy and the patient's immune response. Honey has been used as a wound treatment for millennia. The components responsible for its antibacterial properties are now being elucidated. The study aimed to determine the effects of different concentrations of 'Medihoney' therapeutic honey and Norwegian Forest Honey 1) on the real-time growth of typical chronic wound bacteria; 2) on biofilm formation; and 3) on the same bacteria already embedded in biofilm. Reference strains of MRSE, MRSA, ESBL Klebsiella pneumoniae and Pseudomonas aeruginosa were incubated with dilution series of the honeys in microtitre plates for 20 h. Growth of the bacteria was assessed by measuring optical density every 10 min. Growth curves, biofilm formation and minimum bactericidal concentrations are presented. Both honeys were bactericidal against all the strains of bacteria. Biofilm was penetrated by biocidal substances in honey. Reintroduction of honey as a conventional wound treatment may help improve individual wound care, prevent invasive infections, eliminate colonization, interrupt outbreaks and thereby preserve current antibiotic stocks. SN - 0036-5548 UR - https://www.unboundmedicine.com/medline/citation/19308800/Bacteria_biofilm_and_honey:_a_study_of_the_effects_of_honey_on_'planktonic'_and_biofilm_embedded_chronic_wound_bacteria_ L2 - http://www.tandfonline.com/doi/full/10.1080/00365540902849383 DB - PRIME DP - Unbound Medicine ER -