Tags

Type your tag names separated by a space and hit enter

Muscle-specific differences in the response of mitochondrial proteins to beta-GPA feeding: an evaluation of potential mechanisms.
Am J Physiol Endocrinol Metab. 2009 Jun; 296(6):E1400-8.AJ

Abstract

Beta-Guanadinopropionic acid (beta-GPA) feeding leads to reductions in skeletal muscle phosphagen concentrations and has been used as a tool with which to study the effects of energy charge on skeletal muscle metabolism. Supplementing standard rodent diets with beta-GPA leads to increases in mitochondrial enzyme content in fast but not slow-twitch muscles from male rats. Given this apparent discrepancy between muscle types we used beta-GPA feeding as a model to study signaling pathways involved in mitochondrial biogenesis. We hypothesized that beta-GPA feeding would result in a preferential activation of p38 MAPK and AMPK signaling and reductions in RIP140 protein content in triceps but not soleus muscle. Despite similar reductions in high-energy phosphate concentrations, 6 wk of beta-GPA feeding led to increases in mitochondrial proteins in triceps but not soleus muscles. Differences in the response of mitochondrial proteins to beta-GPA feeding did not seem to be related to a differential activation of p38 MAPK and AMPK signaling pathways or discrepancies in the induction of PPARgamma coactivator (PGC)-1alpha and -1beta. The protein content and expression of the nuclear corepressor RIP140 was reduced in triceps but not soleus muscle. Collectively our results indicate that chronic reductions in high-energy phosphates lead to the activation of p38 MAPK and AMPK signaling and increases in the expression of PGC-1alpha and -1beta in both soleus and triceps muscles. The lack of an effect of beta-GPA feeding on mitochondrial proteins in the soleus muscles could be related to a fiber type-specific effect of beta-GPA on RIP140 protein content.

Authors+Show Affiliations

Alberta Diabetes Institute, 4126C HRIF East, Univ. of Alberta, Edmonton, Alberta, Canada T6G 2E1.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19318515

Citation

Williams, Deon B., et al. "Muscle-specific Differences in the Response of Mitochondrial Proteins to beta-GPA Feeding: an Evaluation of Potential Mechanisms." American Journal of Physiology. Endocrinology and Metabolism, vol. 296, no. 6, 2009, pp. E1400-8.
Williams DB, Sutherland LN, Bomhof MR, et al. Muscle-specific differences in the response of mitochondrial proteins to beta-GPA feeding: an evaluation of potential mechanisms. Am J Physiol Endocrinol Metab. 2009;296(6):E1400-8.
Williams, D. B., Sutherland, L. N., Bomhof, M. R., Basaraba, S. A., Thrush, A. B., Dyck, D. J., Field, C. J., & Wright, D. C. (2009). Muscle-specific differences in the response of mitochondrial proteins to beta-GPA feeding: an evaluation of potential mechanisms. American Journal of Physiology. Endocrinology and Metabolism, 296(6), E1400-8. https://doi.org/10.1152/ajpendo.90913.2008
Williams DB, et al. Muscle-specific Differences in the Response of Mitochondrial Proteins to beta-GPA Feeding: an Evaluation of Potential Mechanisms. Am J Physiol Endocrinol Metab. 2009;296(6):E1400-8. PubMed PMID: 19318515.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Muscle-specific differences in the response of mitochondrial proteins to beta-GPA feeding: an evaluation of potential mechanisms. AU - Williams,Deon B, AU - Sutherland,Lindsey N, AU - Bomhof,Marc R, AU - Basaraba,Susan A U, AU - Thrush,A Brianne, AU - Dyck,David J, AU - Field,Catherine J, AU - Wright,David C, Y1 - 2009/03/24/ PY - 2009/3/26/entrez PY - 2009/3/26/pubmed PY - 2009/7/17/medline SP - E1400 EP - 8 JF - American journal of physiology. Endocrinology and metabolism JO - Am. J. Physiol. Endocrinol. Metab. VL - 296 IS - 6 N2 - Beta-Guanadinopropionic acid (beta-GPA) feeding leads to reductions in skeletal muscle phosphagen concentrations and has been used as a tool with which to study the effects of energy charge on skeletal muscle metabolism. Supplementing standard rodent diets with beta-GPA leads to increases in mitochondrial enzyme content in fast but not slow-twitch muscles from male rats. Given this apparent discrepancy between muscle types we used beta-GPA feeding as a model to study signaling pathways involved in mitochondrial biogenesis. We hypothesized that beta-GPA feeding would result in a preferential activation of p38 MAPK and AMPK signaling and reductions in RIP140 protein content in triceps but not soleus muscle. Despite similar reductions in high-energy phosphate concentrations, 6 wk of beta-GPA feeding led to increases in mitochondrial proteins in triceps but not soleus muscles. Differences in the response of mitochondrial proteins to beta-GPA feeding did not seem to be related to a differential activation of p38 MAPK and AMPK signaling pathways or discrepancies in the induction of PPARgamma coactivator (PGC)-1alpha and -1beta. The protein content and expression of the nuclear corepressor RIP140 was reduced in triceps but not soleus muscle. Collectively our results indicate that chronic reductions in high-energy phosphates lead to the activation of p38 MAPK and AMPK signaling and increases in the expression of PGC-1alpha and -1beta in both soleus and triceps muscles. The lack of an effect of beta-GPA feeding on mitochondrial proteins in the soleus muscles could be related to a fiber type-specific effect of beta-GPA on RIP140 protein content. SN - 0193-1849 UR - https://www.unboundmedicine.com/medline/citation/19318515/Muscle_specific_differences_in_the_response_of_mitochondrial_proteins_to_beta_GPA_feeding:_an_evaluation_of_potential_mechanisms_ L2 - http://www.physiology.org/doi/full/10.1152/ajpendo.90913.2008?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -