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Protease activities in the midgut of Western corn rootworm (Diabrotica virgifera virgifera LeConte).
J Invertebr Pathol. 2009 Mar; 100(3):169-74.JI

Abstract

The Western corn rootworm is one of the most economically important pests in corn. One possibility for controlling this pest is the cultivation of transgenic corn expressing Bacillus thuringiensis (Bt) toxins, such as Cry3A, Cry34Ab1/Cry35Ab1, and Cry3Bb1. However, widespread cultivation of the resulting Bt corn may result in the development of resistant pest populations. The Bt toxins are processed by proteases in the midgut of susceptible insects. Thus, protease activity studies were conducted using the midgut juice (pH 5.75) from third instars larvae of the susceptible Western corn rootworm. As a result, the activities of the serine endopeptidases trypsin, chymotrypsin, elastase, cathepsin G, plasmin, and thrombin; the cysteine endopeptidases cathepsin L, papain, cathepsin B, and cathepsin H; the aspartic endopeptidase pepsin; the metallo endopeptidase saccharolysin; the exopeptidase aminopeptidase, and the omegapeptidase acylaminoacylpeptidase were detected. These results are of basic interest but also lead to reference systems for the identification of protease-mediated resistance mechanisms in potentially resistant individuals.

Authors+Show Affiliations

Federal Research Centre for Cultivated Plants, Julius Kuehn Institute (JKI), Institute for Biological Control, Heinrichstrasse 243, D-64287 Darmstadt, Germany. renate.kaiser-alexnat@jki.bund.de

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19320044

Citation

Kaiser-Alexnat, Renate. "Protease Activities in the Midgut of Western Corn Rootworm (Diabrotica Virgifera Virgifera LeConte)." Journal of Invertebrate Pathology, vol. 100, no. 3, 2009, pp. 169-74.
Kaiser-Alexnat R. Protease activities in the midgut of Western corn rootworm (Diabrotica virgifera virgifera LeConte). J Invertebr Pathol. 2009;100(3):169-74.
Kaiser-Alexnat, R. (2009). Protease activities in the midgut of Western corn rootworm (Diabrotica virgifera virgifera LeConte). Journal of Invertebrate Pathology, 100(3), 169-74. https://doi.org/10.1016/j.jip.2009.01.003
Kaiser-Alexnat R. Protease Activities in the Midgut of Western Corn Rootworm (Diabrotica Virgifera Virgifera LeConte). J Invertebr Pathol. 2009;100(3):169-74. PubMed PMID: 19320044.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protease activities in the midgut of Western corn rootworm (Diabrotica virgifera virgifera LeConte). A1 - Kaiser-Alexnat,Renate, PY - 2009/3/26/entrez PY - 2009/3/26/pubmed PY - 2009/5/27/medline SP - 169 EP - 74 JF - Journal of invertebrate pathology JO - J Invertebr Pathol VL - 100 IS - 3 N2 - The Western corn rootworm is one of the most economically important pests in corn. One possibility for controlling this pest is the cultivation of transgenic corn expressing Bacillus thuringiensis (Bt) toxins, such as Cry3A, Cry34Ab1/Cry35Ab1, and Cry3Bb1. However, widespread cultivation of the resulting Bt corn may result in the development of resistant pest populations. The Bt toxins are processed by proteases in the midgut of susceptible insects. Thus, protease activity studies were conducted using the midgut juice (pH 5.75) from third instars larvae of the susceptible Western corn rootworm. As a result, the activities of the serine endopeptidases trypsin, chymotrypsin, elastase, cathepsin G, plasmin, and thrombin; the cysteine endopeptidases cathepsin L, papain, cathepsin B, and cathepsin H; the aspartic endopeptidase pepsin; the metallo endopeptidase saccharolysin; the exopeptidase aminopeptidase, and the omegapeptidase acylaminoacylpeptidase were detected. These results are of basic interest but also lead to reference systems for the identification of protease-mediated resistance mechanisms in potentially resistant individuals. SN - 1096-0805 UR - https://www.unboundmedicine.com/medline/citation/19320044/Protease_activities_in_the_midgut_of_Western_corn_rootworm__Diabrotica_virgifera_virgifera_LeConte__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2011(09)00016-0 DB - PRIME DP - Unbound Medicine ER -