TY - JOUR T1 - Determination of amlodipine enantiomers in pharmaceuticals using capillary electrophoresis separation and diode array detection. AU - Mikus,P, AU - Maráková,K, AU - Valásková,I, AU - Havránek,E, PY - 2009/3/27/entrez PY - 2009/3/27/pubmed PY - 2009/4/29/medline SP - 76 EP - 9 JF - Die Pharmazie JO - Pharmazie VL - 64 IS - 2 N2 - The present work describes a capillary zone electrophoresis (CZE) separation technique coupled with on-capillary diode array detector (DAD) for highly reliable enantioselective determination of amlodipine (AML) in commercial tablets. For the separation of AML enantiomers, (2-hydroxypropyl)-beta-cyclodextrin (HP-beta-CD) was an appropriate chiral selector providing complete enantioresolution. Optimized separation conditions consisted of 50 mmol/l glycine-acetate buffer, pH 3.2, 50 mg/ml HP-beta-CD. Hydroxyethylcellulose (HEC, 0.2% w/v) served as an electroosmotic flow (EOF) suppressor in this buffer. DAD detection was used for the characterization of the composition of separated zones according to differences in corresponding UV-VIS spectra (scanned in interval 200-800 nm). It was demonstrated, comparing reference and real spectra of the analytes, that the proposed separation method was selective enough to produce pure (non-mixed, i.e. spectrally homogeneous) analyte zones without any interfering compound. Successful validation and application of the proposed CZE-DAD method suggest its routine use in enantioselective control of AML in pharmaceuticals. SN - 0031-7144 UR - https://www.unboundmedicine.com/medline/citation/19320277/Determination_of_amlodipine_enantiomers_in_pharmaceuticals_using_capillary_electrophoresis_separation_and_diode_array_detection_ L2 - https://antibodies.cancer.gov/detail/CPTC-DLC1-1 DB - PRIME DP - Unbound Medicine ER -