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Saturation and comparative mapping of the genomic region harboring Hessian fly resistance gene H26 in wheat.
Theor Appl Genet. 2009 May; 118(8):1589-99.TA

Abstract

Resistance gene H26, derived from Aegilops tauschii Coss., is one of the most effective R genes against the Hessian fly [Mayetiola destructor (Say)], an important pest of wheat (Triticum aestivum L.). Using a limited number of PCR-based molecular markers a previous study mapped H26 to the wheat chromosomal deletion bin 3DL3-0.81-1.00. The objectives of this study were to saturate the chromosomal region harboring H26 with newly developed PCR-based markers and to investigate the collinearity of this wheat chromosomal region with rice (Oryza sativa L.) and Brachypodium distachyon genome. A population of 96 F(2) individuals segregating at the H26 gene locus was used for saturation mapping. All wheat ESTs assigned to the deletion bin 3DL3-0.81-1.00 were used to design STS (sequence tagged site) primers. The wheat ESTs mapped near H26 were further used to BLAST rice and B. distachyon genomic sequences for comparative mapping. To date, 26 newly developed STS markers have been mapped to the chromosomal region spanning the H26 locus. Two of them were mapped 1.0 cM away from the H26 locus. Comparative analysis identified genomic regions on rice chromosome 1 and Brachypodium Super contig 13 which are collinear with the genomic region spanning the H26 locus within the distal region of 3DL. The newly developed STS markers closely linked to H26 will be useful for mapped-based cloning of H26 and marker-assisted selection of this gene in wheat breeding. The results will also enhance understanding of this chromosomal region which contains several other Hessian fly resistance genes.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Yu GT
Department of Entomology, North Dakota State University, Fargo, ND 58105, USA.
Cai X
No affiliation info available
Harris MO
No affiliation info available
Gu YQ
No affiliation info available
Luo MC
No affiliation info available
Xu SS
No affiliation info available

MeSH

AnimalsChromosomes, PlantCrosses, GeneticDipteraExpressed Sequence TagsGenes, PlantGenes, RecessiveGenetic MarkersOryzaPhysical Chromosome MappingPloidiesPoaceaeSequence Tagged SitesSpecies SpecificityTriticum

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

19322558

Citation

Yu, Guo Tai, et al. "Saturation and Comparative Mapping of the Genomic Region Harboring Hessian Fly Resistance Gene H26 in Wheat." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 118, no. 8, 2009, pp. 1589-99.
Yu GT, Cai X, Harris MO, et al. Saturation and comparative mapping of the genomic region harboring Hessian fly resistance gene H26 in wheat. Theor Appl Genet. 2009;118(8):1589-99.
Yu, G. T., Cai, X., Harris, M. O., Gu, Y. Q., Luo, M. C., & Xu, S. S. (2009). Saturation and comparative mapping of the genomic region harboring Hessian fly resistance gene H26 in wheat. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 118(8), 1589-99. https://doi.org/10.1007/s00122-009-1006-5
Yu GT, et al. Saturation and Comparative Mapping of the Genomic Region Harboring Hessian Fly Resistance Gene H26 in Wheat. Theor Appl Genet. 2009;118(8):1589-99. PubMed PMID: 19322558.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Saturation and comparative mapping of the genomic region harboring Hessian fly resistance gene H26 in wheat. AU - Yu,Guo Tai, AU - Cai,Xiwen, AU - Harris,Marion O, AU - Gu,Yong Qiang, AU - Luo,Ming-Cheng, AU - Xu,Steven S, Y1 - 2009/03/26/ PY - 2008/09/26/received PY - 2009/03/08/accepted PY - 2009/3/27/entrez PY - 2009/3/27/pubmed PY - 2009/9/3/medline SP - 1589 EP - 99 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 118 IS - 8 N2 - Resistance gene H26, derived from Aegilops tauschii Coss., is one of the most effective R genes against the Hessian fly [Mayetiola destructor (Say)], an important pest of wheat (Triticum aestivum L.). Using a limited number of PCR-based molecular markers a previous study mapped H26 to the wheat chromosomal deletion bin 3DL3-0.81-1.00. The objectives of this study were to saturate the chromosomal region harboring H26 with newly developed PCR-based markers and to investigate the collinearity of this wheat chromosomal region with rice (Oryza sativa L.) and Brachypodium distachyon genome. A population of 96 F(2) individuals segregating at the H26 gene locus was used for saturation mapping. All wheat ESTs assigned to the deletion bin 3DL3-0.81-1.00 were used to design STS (sequence tagged site) primers. The wheat ESTs mapped near H26 were further used to BLAST rice and B. distachyon genomic sequences for comparative mapping. To date, 26 newly developed STS markers have been mapped to the chromosomal region spanning the H26 locus. Two of them were mapped 1.0 cM away from the H26 locus. Comparative analysis identified genomic regions on rice chromosome 1 and Brachypodium Super contig 13 which are collinear with the genomic region spanning the H26 locus within the distal region of 3DL. The newly developed STS markers closely linked to H26 will be useful for mapped-based cloning of H26 and marker-assisted selection of this gene in wheat breeding. The results will also enhance understanding of this chromosomal region which contains several other Hessian fly resistance genes. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/19322558/Saturation_and_comparative_mapping_of_the_genomic_region_harboring_Hessian_fly_resistance_gene_H26_in_wheat_ L2 - https://dx.doi.org/10.1007/s00122-009-1006-5 DB - PRIME DP - Unbound Medicine ER -